Zobrazeno 1 - 8 of 8 pro vyhledávání: '"Jakob Ulstrup"'
1
Structure and autoregulation of a P4-ATPase lipid flippase
Autor: Thomas Boesen, Cédric Montigny, Dovile Januliene, Joseph A. Lyons, Arne Möller, Poul Nissen, Milena Timcenko, Werner Kühlbrandt, Guillaume Lenoir, Thibaud Dieudonné, Jesper Lykkegaard Karlsen, Jakob Ulstrup, Miriam-Rose Ash
Publikováno v: Timcenko, M, Lyons, J A, Januliene, D, Ulstrup, J J, Dieudonné, T, Montigny, C, Ash, M R, Karlsen, J L, Boesen, T, Kühlbrandt, W, Lenoir, G, Moeller, A & Nissen, P 2019, ' Structure and autoregulation of a P4-ATPase lipid flippase ', Nature, vol. 571, no. 7765, pp. 366-370 . https://doi.org/10.1038/s41586-019-1344-7
Nature
Nature, Nature Publishing Group, 2019, ⟨10.1038/s41586-019-1344-7⟩
Nature, 2019, ⟨10.1038/s41586-019-1344-7⟩
International audience; Type 4 P-type ATPases (P4-ATPases) are lipid flippases that drive the active transport of phospholipids from exoplasmic or luminal leaflets to cytosolic leaflets of eukaryotic membranes. The molecular architecture of P4-ATPase
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4978c31dd501da147b54cf898ba06b00
https://doi.org/10.1038/s41586-019-1344-7
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2
High phosphatidylinositol 4-phosphate (PI4P)-dependent ATPase activity for the Drs2p-Cdc50p flippase after removal of its N- and C-terminal extensions
Autor: Philippe Champeil, Pierre Le Maréchal, Joseph A. Lyons, Poul Nissen, José Luis Vázquez-Ibar, Cédric Montigny, Aurore Jacquot, Thibaud Dieudonné, Jakob Ulstrup, Miriam-Rose Ash, Guillaume Lenoir, Hassina Azouaoui
Publikováno v: Journal of Biological Chemistry
Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2017, 292 (19), pp.7954-7970. ⟨10.1074/jbc.M116.751487⟩
Journal of Biological Chemistry, 2017, 292 (19), pp.7954-7970. ⟨10.1074/jbc.M116.751487⟩
Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2017, 292 (19), pp.7954-7970. 〈10.1074/jbc.M116.751487〉
Azouaoui, H, Montigny, C, Dieudonné, T, Champeil, P, Jacquot, A, Vázquez-Ibar, J L, le Maréchal, P, Ulstrup, J, Ash, M-R, Lyons, J A, Nissen, P & Lenoir, G 2017, ' A High and Phosphatidylinositol-4-phosphate (PI4P)-dependent ATPase Activity for the Drs2p/Cdc50p Flippase after Removal of its N-and C-terminal Extensions ', Journal of Biological Chemistry, vol. 292, pp. 7954-7970 . https://doi.org/10.1074/jbc.M116.751487
International audience; P4-ATPases, also known as phospholipid flippases, are responsible for creating and maintaining transbilayer lipid asymmetry in eukaryotic cell membranes. Here, we use limited proteolysis to investigate the role of the N and C
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::78a13bfa67cbd26904683eacf27edde2
https://hal.archives-ouvertes.fr/hal-01697714
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4
Against the odds? De novo structure determination of a pilin with two cysteine residues by sulfur SAD
Autor: Jakob Ulstrup, Poul Nissen, Manuela Gorgel, Manfred S. Weiss, Thomas Boesen, Andreas Bøggild, Uwe Müller
Publikováno v: Gorgel, M, Bøggild, A, Ulstrup, J, Weiss, M S, Müller, U, Nissen, P & Boesen, T 2015, ' Against the odds? De novo structure determination of a pilin with two cysteine residues by sulfur SAD ', Acta crystallographica Section D: Structural biology, vol. 71, no. 5, pp. 1095-1101 . https://doi.org/10.1107/S1399004715003272
Exploiting the anomalous signal of the intrinsic S atoms to phase a protein structure is advantageous, as ideally only a single well diffracting native crystal is required. However, sulfur is a weak anomalous scatterer at the typical wavelengths used
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d5014bc7764d9033de972c756b1c5351
https://pure.au.dk/portal/da/publications/against-the-odds-de-novo-structure-determination-of-a-pilin-with-two-cysteine-residues-by-sulfur-sad(2e61cce6-58bf-49aa-a26a-b349132fffc3).html
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5
High-resolution structure of a type IV pilin from the metal-reducing bacterium Shewanella oneidensis
Autor: Jakob Ulstrup, Thomas Boesen, Manuela Gorgel, Poul Nissen, Søren Vrønning Hoffmann, Nykola C. Jones, Andreas Bøggild
Publikováno v: BMC Structural Biology
Gorgel, M, Ulstrup, J, Bøggild, A, Jones, N C, Hoffmann, S V, Nissen, P & Boesen, T 2015, ' High-resolution structure of a type IV pilin from the metal-reducing bacterium Shewanella oneidensis ', BMC Biochemistry and Structural Biology, vol. 15, no. 4, pp. 1-17 . https://doi.org/10.1186/s12900-015-0031-7
Background Type IV pili are widely expressed among Gram-negative bacteria, where they are involved in biofilm formation, serve in the transfer of DNA, motility and in the bacterial attachment to various surfaces. Type IV pili in Shewanella oneidensis
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d57e1713fdede7437f8bea4660db8ff
https://pubmed.ncbi.nlm.nih.gov/25886849
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6
Sulfur-SAD phasing and UV-RIP analysis on a single Cysteine bridge protein
Autor: Jakob Ulstrup, Uwe Mueller, Manuela Gorgel, Poul Nissen, Andreas Bøggild, Thomas Boesen, Manfred S. Weiss
Publikováno v: Acta Crystallographica Section A Foundations and Advances. 70:C602-C602
The advantage of using the anomalous signal of sulfur for phase determination is that only a single, well-diffracting crystal is needed and that a native structure will be obtained. Using long-wavelength S-SAD to a resolution of 1.9 Å we have determ
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::eff5c1f71d159f413bb19252d44f8569
https://doi.org/10.1107/s2053273314093978
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