Zobrazeno 1 - 10
of 120
pro vyhledávání: '"Jakob Reiser"'
CRISPR library screening to develop HEK293-derived cell lines with improved lentiviral vector titers
Publikováno v:
Frontiers in Genome Editing, Vol 5 (2023)
Lentiviral (LV) vectors have emerged as powerful tools for treating genetic and acquired human diseases. As clinical studies and commercial demands have progressed, there has been a growing need for large amounts of purified LV vectors. To help meet
Externí odkaz:
https://doaj.org/article/37bb34bf934f49fa9655358b512911da
Autor:
Takele Argaw, Michael P. Marino, Andrew Timmons, Lindsey Eldridge, Kazuyo Takeda, Pingjuan Li, Anna Kwilas, Wu Ou, Jakob Reiser
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 21, Iss , Pp 670-680 (2021)
Despite their exceptional capacity for transgene delivery ex vivo, lentiviral (LV) vectors have been slow to demonstrate clinical utility in the context of in vivo applications. Unresolved safety concerns related to broad LV vector tropism have limit
Externí odkaz:
https://doaj.org/article/bb1aa2c91590406b93d2703125bf4eaa
Autor:
Dahae Hailey Bae, Michael Marino, Brian Iaffaldano, Sydney Fenstermaker, Sandra Afione, Takele Argaw, Jacob McCright, Anna Kwilas, John A. Chiorini, Andrew E. Timmons, Jakob Reiser
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 18, Iss , Pp 631-638 (2020)
The use of the human embryonic kidney (HEK) 293T cell line to manufacture vectors for in vivo applications raises safety concerns due to the presence of SV40 T antigen-encoding sequences. We used CRISPR-Cas9 genome editing to remove the SV40 T antige
Externí odkaz:
https://doaj.org/article/8da80acc707540e8a11da1b98006448b
Publikováno v:
PLoS ONE, Vol 8, Iss 5, p e64389 (2013)
Gene regulation remains one of the major challenges for gene therapy in clinical trials. In the present study, we first generated a binary tetracycline-on (Tet-On) system based on two lentivirus vectors, one expressing both human glial cell line-deri
Externí odkaz:
https://doaj.org/article/f91a1fc8898c49a0b980b0d405bce09d
Publikováno v:
PLoS ONE, Vol 8, Iss 11, p e81131 (2013)
The in vitro differentiation of human induced pluripotent stem cells (hiPSC) to generate specific types of cells is inefficient, and the remaining undifferentiated cells may form teratomas. This raises safety concerns for clinical applications of hiP
Externí odkaz:
https://doaj.org/article/48c0fcea78e74d5ea8d92d01c109fd6b
Autor:
Takele Argaw, Jacob McCright, Dahae Hailey Bae, Andrew E. Timmons, Jakob Reiser, Michael P. Marino, Anna Kwilas, John A. Chiorini, Sandra Afione, Brian Iaffaldano, Sydney Fenstermaker
Publikováno v:
Molecular Therapy. Methods & Clinical Development
Molecular Therapy: Methods & Clinical Development, Vol 18, Iss, Pp 631-638 (2020)
Molecular Therapy: Methods & Clinical Development, Vol 18, Iss, Pp 631-638 (2020)
The use of the human embryonic kidney (HEK) 293T cell line to manufacture vectors for in vivo applications raises safety concerns due to the presence of SV40 T antigen-encoding sequences. We used CRISPR-Cas9 genome editing to remove the SV40 T antige
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a9effa30ccabbae255fd9044075e9736
http://europepmc.org/articles/PMC7397404
http://europepmc.org/articles/PMC7397404
Publikováno v:
International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 22, Iss 10263, p 10263 (2021)
International Journal of Molecular Sciences, Vol 22, Iss 10263, p 10263 (2021)
Lentiviral (LV) vectors have emerged as powerful tools for transgene delivery ex vivo but in vivo gene therapy applications involving LV vectors have faced a number of challenges, including the low efficiency of transgene delivery, a lack of tissue s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e24033af0ae1d45195cc198a93fd9189
https://pubmed.ncbi.nlm.nih.gov/34638603
https://pubmed.ncbi.nlm.nih.gov/34638603
Autor:
Brian Iaffaldano, Jakob Reiser
Publikováno v:
International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 22, Iss 857, p 857 (2021)
International Journal of Molecular Sciences, Vol 22, Iss 857, p 857 (2021)
Directed evolution is a powerful approach for protein engineering and functional studies. However, directed evolution outputs from bacterial and yeast systems do not always translate to higher organisms. In situ directed evolution in plant and animal
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::322aad1fcb6ba54f60604452208b8059
https://pubmed.ncbi.nlm.nih.gov/33467049
https://pubmed.ncbi.nlm.nih.gov/33467049
Autor:
Eric Laborde, Shams Halat, Michael P. Marino, Li Li, Xin Zhang, Linh Hellmers, Maria Latsis, M'Liss A. Hudson, Daniel Canter, Ravan Moret, Marc R. Matrana, Jessie Gills, Sunil Talwar, Grace Maresh, Maureen Shuh, John Nelson, Jakob Reiser, Stephen Bardot
Publikováno v:
Oncotarget
// Jessie Gills 1, * , Ravan Moret 2, * , Xin Zhang 2 , John Nelson 1 , Grace Maresh 2 , Linh Hellmers 2 , Daniel Canter 1 , M’Liss Hudson 1, 6 , Shams Halat 3 , Marc Matrana 4 , Michael P. Marino 5 , Jakob Reiser 5 , Maureen Shuh 2 , Eric Laborde
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0f7e6816b0d6fddf7facda219d5fe6ba
http://europepmc.org/articles/PMC6135689
http://europepmc.org/articles/PMC6135689
Autor:
Michael P. Marino, Jakob Reiser, Takele Argaw, Jizhong Zou, Pingjuan Li, Brian Iaffaldano, Michael T Morreale
Publikováno v:
Human Gene Therapy Methods. 29:135-145
The adeno-associated virus serotype 2 (AAV2) Rep 78 protein, a strand-specific endonuclease (nickase) promotes site-specific integration of transgene sequences bearing homology arms corresponding to the AAVS1 safe harbor locus. To investigate the eff
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5d37229f636adbcc0580d5a3533c3689
https://doi.org/10.1089/hgtb.2018.052
https://doi.org/10.1089/hgtb.2018.052