Regulating the timing of enhancer transitions is key to defining sharp boundaries of Fushi tarazu expression in the Drosophila embryo

Autor: Anthony Birnie, Audrey Plat, Jacques P. Bothma

SummaryCoordinating the action of different enhancers is crucial to correctly specify cell fate decisions during development. Yet it remains poorly understood how the activity of multiple enhancers is choregraphed in time. To shed light on this quest

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::8b490ceb1b3cc1e7f910a6622ca8a2de
https://doi.org/10.1101/2022.09.27.509025

Long-range intra-protein communication can be transmitted by correlated side-chain fluctuations alone.

Autor: Kateri H Dubay, Jacques P Bothma, Phillip L Geissler

Publikováno v: PLoS Computational Biology, Vol 7, Iss 9, p e1002168 (2011)

Allosteric regulation is a key component of cellular communication, but the way in which information is passed from one site to another within a folded protein is not often clear. While backbone motions have long been considered essential for long-ra

Externí odkaz: https://doaj.org/article/ed0f07843e564972a972033c89a1e8cb

The Snail Repressor Inhibits Release, Not Elongation, of Paused Pol II in the Drosophila Embryo

Autor: Jacques P. Bothma, Joe Magliocco, Michael Levine

Publikováno v: Current Biology. 21(18):1571-1577

Summary The development of the precellular Drosophila embryo is characterized by exceptionally rapid transitions in gene activity, with broadly distributed maternal regulatory gradients giving way to precise on/off patterns of gene expression within

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::268d9e0732e76ae214d6d2015e3deef9

Enhancer additivity and non-additivity are determined by enhancer strength in the Drosophila embryo

Autor: Michael Levine, Thomas Gregor, Hernan G. Garcia, Samuel Ng, Michael Perry, Jacques P. Bothma

Publikováno v: eLife
eLife, vol 4, iss AUGUST2015
eLife, Vol 4 (2015)
Bothma, Jacques P; Garcia, Hernan G; Ng, Samuel; Perry, Michael W; Gregor, Thomas; & Levine, Michael. (2015). Enhancer additivity and non-additivity are determined by enhancer strength in the Drosophila embryo.. eLife, 4. doi: 10.7554/eLife.07956. UC San Diego: Retrieved from: http://www.escholarship.org/uc/item/9hq3v597
eLife, vol 4
Bothma, JP; Garcia, HG; Ng, S; Perry, MW; Gregor, T; & Levine, M. (2015). Enhancer additivity and non-additivity are determined by enhancer strength in the Drosophila embryo. eLife, 4(AUGUST2015). doi: 10.7554/eLife.07956.001. UC Berkeley: Retrieved from: http://www.escholarship.org/uc/item/6h3183kh

Metazoan genes are embedded in a rich milieu of regulatory information that often includes multiple enhancers possessing overlapping activities. In this study, we employ quantitative live imaging methods to assess the function of pairs of primary and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9457f996580d406e97c2b738cb35cf09
http://europepmc.org/articles/PMC4532966

Dynamic regulation of eve stripe 2 expression reveals transcriptional bursts in living Drosophila embryos

Autor: Jacques P. Bothma, Thomas Gregor, Hernan G. Garcia, Emilia Esposito, Michael Levine, Gavin Schlissel

Publikováno v: Bothma, JP; Garcia, HG; Esposito, E; Schlissel, G; Gregor, T; & Levine, M. (2014). Dynamic regulation of eve stripe 2 expression reveals transcriptional bursts in living Drosophila embryos. Proceedings of the National Academy of Sciences of the United States of America, 111(29), 10598-10603. doi: 10.1073/pnas.1410022111. UC Berkeley: Retrieved from: http://www.escholarship.org/uc/item/09n4v2k6
Proceedings of the National Academy of Sciences of the United States of America, vol 111, iss 29

We present the use of recently developed live imaging methods to examine the dynamic regulation of even-skipped (eve) stripe 2 expression in the precellular Drosophila embryo. Nascent transcripts were visualized via MS2 RNA stem loops. The eve stripe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff49f509add95c2d7c09c673b10794a9
http://www.escholarship.org/uc/item/09n4v2k6