Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Jacqueline N. Watson"'
Autor:
Matthew C. Deen, Fahimeh S. Shidmoossavee, Saeideh Shamsi Kazem Abadi, Andrew J. Bennet, Jacqueline N. Watson
Publikováno v:
Glycobiology. 30(5)
N-acetylneuraminic acid (5-acetamido-3,5-dideoxy-d-glycero-d-galacto-non-2-ulosonic acid), which is the principal sialic acid family member of the non-2-ulosonic acids and their various derivatives, is often found at the terminal position on the glyc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::228121a89b874fa035df01a348f29f2f
https://pubmed.ncbi.nlm.nih.gov/31804700
https://pubmed.ncbi.nlm.nih.gov/31804700
Autor:
Andrew J. Bennet, Jefferson Y. Chan, Thor J. Borgford, Jacqueline N. Watson, April Lu, Viviana C. Cerda
Publikováno v:
Biochemistry. 51:433-441
Mutagenesis of the conserved glutamic acid of influenza type A (E277) and Micromonospora viridifaciens (E260) sialidases was performed to probe the contribution of this strictly conserved residue to catalysis. Kinetic studies of the E260D and E260C M
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc77be54d95fe9c576bd48f4ffc4a011
https://doi.org/10.1021/bi201019n
https://doi.org/10.1021/bi201019n
Publikováno v:
Glycobiology. 17:401-410
Aspergillus fumigatus is an opportunistic fungal pathogen that causes a life-threatening invasive fungal disease (invasive aspergillosis, IA) in immunocompromised individuals. The first step of pathogenesis is thought to be the attachment of conidia
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a7ce2831f75ed9856afcd0fd0ad47b8a
https://doi.org/10.1093/glycob/cwl085
https://doi.org/10.1093/glycob/cwl085
Publikováno v:
Biochemistry. 45:13264-13275
The Y370G inverting mutant sialidase from Micromonospora viridifaciens possesses beta-sialidase activity with phenyl beta-sialoside (Ph-betaNeuAc) to give alpha-sialic acid as the first formed product. The derived catalytic rate constants for k(cat)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1e9c620224be50253687bc526305c8f1
https://doi.org/10.1021/bi061489x
https://doi.org/10.1021/bi061489x
Publikováno v:
Biochemistry. 45:9319-9326
The sialidase from Micromonospora viridifaciens has been found to catalyze the hydrolysis of aryl 2-thio-alpha-D-sialosides with remarkable efficiency: the first- and second-order rate constants, kcat and kcat/Km, for the enzyme-catalyzed hydrolysis
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e5393a4a78c95330657b6728da04156b
https://doi.org/10.1021/bi0607507
https://doi.org/10.1021/bi0607507
Publikováno v:
Biochemistry. 44:9117-9122
Mutagenesis of the conserved tyrosine (Y370) of the Micromonospora viridifaciens sialidase to small amino acids changes the mechanism of catalysis from retention of anomeric configuration to inversion [Watson, J. N., et al. (2003) Biochemistry 42, 12
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2f3c7b4b422e76ef7880b2e9ab9bade3
https://doi.org/10.1021/bi050517t
https://doi.org/10.1021/bi050517t
Autor:
Doug T. H. Chou, Johnny H Chen, Jacqueline N. Watson, Andrew J. Bennet, Thor J. Borgford, Tara L. Knoll
Publikováno v:
Biochemistry and Cell Biology. 83:115-122
Investigations into subtle changes in the catalytic activity of sialidases have been performed using enzymes from several different origins, and their results have been compared. This work highlights the potential pitfalls encountered when extending
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::70b6be3fa7c247a9557ab7f84242691d
https://doi.org/10.1139/o04-126
https://doi.org/10.1139/o04-126
Autor:
Andrew J. Bennet, Doug T. H. Chou, Andrew A. Scholte, Thor J. Borgford,‡ and, Jacqueline N. Watson
Publikováno v:
Journal of the American Chemical Society. 122:8357-8364
A reagent panel, comprised of five pyridinium salts of α-d-N-acetylneuraminic acid, was synthesized and then used to probe enzymatic (α-sialidase) and nonenzymatic mechanisms of neuraminide hydroly...
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4ea9612c854f961d1239ce2ae1fdf2e9
https://doi.org/10.1021/ja001641x
https://doi.org/10.1021/ja001641x
Publikováno v:
Biochemistry. 49(30)
The Micromonospora viridifaciens Y370G inverting mutant sialidase has been found to possess beta-sialidase activity with various fluoro-substituted phenyl beta-sialosides. A reagent panel of seven mono- and difluorophenyl beta-d-sialosides was synthe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dadb485b53430ab8e330fdd395e67f29
https://pubmed.ncbi.nlm.nih.gov/20575524
https://pubmed.ncbi.nlm.nih.gov/20575524
Publikováno v:
Organicbiomolecular chemistry. 4(24)
Two isomeric 4-methylumbelliferyl-alpha-D-N-acetylneuraminylgalactopyranosides (1 and 2) were synthesised. These compounds contain either the natural alpha-2,3 or alpha-2,6 sialyl-galactosyl linkages, as well as an attached 4-methylumbelliferone for
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d739bce8ed70064c85bc3eb2b635030f
https://pubmed.ncbi.nlm.nih.gov/17268638
https://pubmed.ncbi.nlm.nih.gov/17268638