MukBEF-dependent chromosomal organization in widened Escherichia coli

Autor: Aleksandre Japaridze, Raman van Wee, Christos Gogou, Jacob W. J. Kerssemakers, Daan F. van den Berg, Cees Dekker

Publikováno v: Frontiers in Microbiology, Vol 14 (2023)

The bacterial chromosome is spatially organized through protein-mediated compaction, supercoiling, and cell-boundary confinement. Structural Maintenance of Chromosomes (SMC) complexes are a major class of chromosome-organizing proteins present throug

Externí odkaz: https://doaj.org/article/80918bae145f47938a41e92a3e2c3f82

Direct imaging of the circular chromosome in a live bacterium

Autor: Fabai Wu, Aleksandre Japaridze, Xuan Zheng, Jakub Wiktor, Jacob W. J. Kerssemakers, Cees Dekker

Publikováno v: Nature Communications, Vol 10, Iss 1, Pp 1-9 (2019)

Bacterial chromosomes are tightly packed, limiting structural analysis by imaging techniques. Here, by quantitative time-lapse single-cell imaging of widened Escherichia coli cells, Wu and Japaridze et al. show that the chromosome exhibits a ring-lik

Externí odkaz: https://doaj.org/article/5820c2a17604433989895a5623404756

Condensin extrudes DNA loops in steps up to hundreds of base pairs that are generated by ATP binding events

Autor: Jacob W. J. Kerssemakers, Sang-Hyun Rah, Richard Janissen, Je-Kyung Ryu, Davide Michieletto, Cees Dekker, Andrea Bonato

Publikováno v: Ryu, J-K, Rah, S-H, Janissen, R, Kerssemakers, J W J, Bonato, A, Michieletto, D & Dekker, C 2022, ' Condensin extrudes DNA loops in steps up to hundreds of base pairs that are generated by ATP binding events ', Nucleic Acids Research, vol. 50, no. 2, pp. 820-832 . https://doi.org/10.1093/nar/gkab1268
Nucleic acids research, 50(2)
Nucleic Acids Research
bioRxiv
Nucl. Acid Res. 50

The condensin SMC protein complex organizes chromosomal structure by extruding loops of DNA. Its ATP-dependent motor mechanism remains unclear but likely involves steps associated with large conformational changes within the ∼50 nm protein complex.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b589d734e704ed6b5292c013bcddcc0e
https://www.pure.ed.ac.uk/ws/files/261761699/gkab1268.pdf

Direct imaging of the circular chromosome in a live bacterium

Autor: Cees Dekker, Xuan Zheng, Jakub Wiktor, Fabai Wu, Aleksandre Japaridze, Jacob W. J. Kerssemakers

Publikováno v: Nature Communications, Vol 10, Iss 1, Pp 1-9 (2019)
Nature Communications
Nature Communications, 10(1)

Although the physical properties of chromosomes, including their morphology, mechanics, and dynamics are crucial for their biological function, many basic questions remain unresolved. Here we directly image the circular chromosome in live E. coli wit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e323d0efb74027d806fa68b2d54e724
http://link.springer.com/article/10.1038/s41467-019-10221-0

Scanning a DNA molecule for bound proteins using hybrid magnetic and optical tweezers.

Autor: Marijn T J van Loenhout, Iwijn De Vlaminck, Benedetta Flebus, Johan F den Blanken, Ludovit P Zweifel, Koen M Hooning, Jacob W J Kerssemakers, Cees Dekker

Publikováno v: PLoS ONE, Vol 8, Iss 6, p e65329 (2013)

The functional state of the genome is determined by its interactions with proteins that bind, modify, and move along the DNA. To determine the positions and binding strength of proteins localized on DNA we have developed a combined magnetic and optic

Externí odkaz: https://doaj.org/article/0f3172dcf1a24a7a9a8a5e86a6f96c3c