The cancer angiogenesis co-culture assay: In vitro quantification of the angiogenic potential of tumoroids.

Autor: Sarah Line Bring Truelsen, Nabi Mousavi, Haoche Wei, Lucy Harvey, Rikke Stausholm, Erik Spillum, Grith Hagel, Klaus Qvortrup, Ole Thastrup, Henrik Harling, Harry Mellor, Jacob Thastrup

Publikováno v: PLoS ONE, Vol 16, Iss 7, p e0253258 (2021)

The treatment response to anti-angiogenic agents varies among cancer patients and predictive biomarkers are needed to identify patients with resistant cancer or guide the choice of anti-angiogenic treatment. We present "the Cancer Angiogenesis Co-Cul

Externí odkaz: https://doaj.org/article/5310916ac9324f84825d18d85b90f7db

Short-term spheroid culture of primary colorectal cancer cells as an in vitro model for personalizing cancer medicine.

Autor: Maria Jeppesen, Grith Hagel, Anders Glenthoj, Ben Vainer, Per Ibsen, Henrik Harling, Ole Thastrup, Lars N Jørgensen, Jacob Thastrup

Publikováno v: PLoS ONE, Vol 12, Iss 9, p e0183074 (2017)

Chemotherapy treatment of cancer remains a challenge due to the molecular and functional heterogeneity displayed by tumours originating from the same cell type. The pronounced heterogeneity makes it difficult for oncologists to devise an effective th

Externí odkaz: https://doaj.org/article/9df1d329274e465686b17eb5dcc4db76

In Vitro Coculture Assays of Angiogenesis

Autor: Haoche, Wei, Ananthalakshmy, Sundararaman, Sarah Line Bring, Truelsen, David, Gurevich, Jacob, Thastrup, Harry, Mellor

Publikováno v: Methods in molecular biology (Clifton, N.J.). 2206

During angiogenesis, endothelial cells must undergo a coordinated set of morphological changes in order to form a new vessel. There is a need for endothelial cells to communicate with each other in order to take up different identities in the sprout

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::f36800d2f2b0b4754b8485def3da695d
https://pubmed.ncbi.nlm.nih.gov/32754809

KRAS mutations in the parental tumour accelerate in vitro growth of tumoroids established from colorectal adenocarcinoma

Autor: Vera Timmermans, Linea Cecilie Melchior, Jacob Thastrup, Nabi Mousavi, Steffen Heegaard, Anna Hammerich Thysen, Sarah Line Bring Truelsen, Henrik Harling, Lars N. Jorgensen, Grith Hagel

Publikováno v: Mousavi, N, Truelsen, S L B, Hagel, G, Jorgensen, L N, Harling, H, Timmermans, V, Melchior, L C, Thysen, A H, Heegaard, S & Thastrup, J 2019, ' KRAS mutations in the parental tumour accelerate in vitro growth of tumoroids established from colorectal adenocarcinoma ', International Journal of Experimental Pathology, vol. 100, no. 1, pp. 12-18 . https://doi.org/10.1111/iep.12308

The aim of the present study was to characterize a patient-derived in vitro 3D model (ie tumoroid) established from colorectal adenocarcinoma. This study investigated the growth rate of tumoroids and whether the Kirsten rat sarcoma (KRAS) mutations i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::be64c15c4a4732c8bdd8121194ad1f5d
https://europepmc.org/articles/PMC6463397/

Characterization of genetic intratumor heterogeneity in colorectal cancer and matching patient-derived spheroid cultures

Autor: Michael Knudsen, Jacob Thastrup, Mogens Madsen, Philippe Lamy, Sigrid S. Árnadóttir, Maria Jeppesen, Iver Nordentoft, Søren Vang, Ole Thastrup, Claus L. Andersen, Jesper B. Bramsen

Publikováno v: Molecular Oncology
Árnadóttir, S S, Jeppesen, M, Lamy, P, Bramsen, J B, Nordentoft, I, Knudsen, M, Vang, S, Madsen, M R, Thastrup, O, Thastrup, J & L Andersen, C 2018, ' Characterization of genetic intratumor heterogeneity in colorectal cancer and matching patient-derived spheroid cultures ', Molecular Oncology, vol. 12, pp. 132-147 . https://doi.org/10.1002/1878-0261.12156
Molecular Oncology, Vol 12, Iss 1, Pp 132-147 (2018)
Árnadóttir, S S, Jeppesen, M, Lamy, P, Bramsen, J B, Nordentoft, I, Knudsen, M, Vang, S, Madsen, M R, Thastrup, O, Thastrup, J & L Andersen, C 2018, ' Characterization of genetic intratumor heterogeneity in colorectal cancer and matching patient-derived spheroid cultures ', Molecular Oncology, vol. 12, no. 1, pp. 132–147 . https://doi.org/10.1002/1878-0261.12156

Patient-derived in vitro cultures of colorectal cancer (CRC) may help guide treatment strategies prior to patient treatment. However, most previous studies have been performed on a single biopsy per tumor. The purpose of this study was to analyze mul

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::16e85a97b11ed37e1f3f8435836ac888
https://pubmed.ncbi.nlm.nih.gov/29130628