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pro vyhledávání: '"Jacob T Whitt"'
Autor:
Peter J Enyeart, Steven M Chirieleison, Mai N Dao, Jiri Perutka, Erik M Quandt, Jun Yao, Jacob T Whitt, Adrian T Keatinge‐Clay, Alan M Lambowitz, Andrew D Ellington
Publikováno v:
Molecular Systems Biology, Vol 9, Iss 1, Pp 1-16 (2013)
Abstract Efficient bacterial genetic engineering approaches with broad‐host applicability are rare. We combine two systems, mobile group II introns (‘targetrons’) and Cre/lox, which function efficiently in many different organisms, into a versa
Externí odkaz:
https://doaj.org/article/a6684a167997479bb650e58ad13f1786
Autor:
Steven M. Chirieleison, Peter J. Enyeart, Jiri Perutka, Erik M. Quandt, Mai N Dao, Jacob T Whitt, Alan M. Lambowitz, Adrian T. Keatinge-Clay, Andrew D. Ellington, Jun Yao
Publikováno v:
Molecular Systems Biology
A general bacterial genome engineering framework, ‘Genome Editing via Targetrons and Recombinases' (GETR), is presented. GETR combines mobile group II introns (targetrons) and the Cre/lox system to allow genomic manipulations at a large scale.
Autor:
Patrick Shiflett, Adin Pemberton, Ryan M. Kramer, Deborah Taylor, Jiri Perutka, Jacob T Whitt, Alan M. Lambowitz, Roland Saldanha, Thomas J. Lamkin, Andrew D. Ellington
Publikováno v:
BMC Biotechnology
Background Anthrax is a zoonotic disease recognized to affect herbivores since Biblical times and has the widest range of susceptible host species of any known pathogen. The ease with which the bacterium can be weaponized and its recent deliberate us
Publikováno v:
Biochemistry. 45(41)
The mobile Lactococcus lactis Ll.LtrB group II intron integrates into DNA target sites by a mechanism in which the intron RNA reverse splices into one DNA strand, while the intron-encoded protein uses a C-terminal DNA endonuclease domain to cleave th
Autor:
Mehdi Moini, Jacob T Whitt
Publikováno v:
Analytical chemistry. 75(9)
A new capillary electrophoresis interface to electrospray ionization mass spectrometry (CE/ESI-MS) is introduced in which the electrical connection to the CE capillary outlet/ESI electrode is achieved by transfer of small ions related to the backgrou