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of 13
pro vyhledávání: '"Jacob L, Litke"'
Autor:
Jacob L, Litke, Samie R, Jaffrey
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2570
RNA aptamers can be genetically encoded in cells to probe and manipulate cellular function. The usefulness of aptamers in mammalian cells is limited by low accumulation and degradation by ribonucleases. Expression of circular RNA aptamers using the T
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::b30fb99a6a848a193f99e693fde142ed
https://pubmed.ncbi.nlm.nih.gov/36156786
https://pubmed.ncbi.nlm.nih.gov/36156786
Autor:
Jacob L. Litke, Samie R. Jaffrey
Publikováno v:
Methods in Molecular Biology ISBN: 9781071626948
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::249244843b86ff011d22d00450ec9b1b
https://doi.org/10.1007/978-1-0716-2695-5_17
https://doi.org/10.1007/978-1-0716-2695-5_17
Publikováno v:
Journal of the American Chemical Society. 142:14117-14124
To understand the role of intracellular metabolites in cellular processes, it is important to measure the dynamics and fluxes of small molecules in living cells. Although conventional metabolite sensors composed of fluorescent proteins have been made
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3e60fb6f9b9078db982c2bf268d7b67c
https://doi.org/10.1021/jacs.0c02931
https://doi.org/10.1021/jacs.0c02931
Publikováno v:
Angew Chem Int Ed Engl
Spinach and Broccoli are fluorogenic RNA aptamers that bind and activate the fluorescence of DFHBI, a mimic of the green fluorescence protein chromophore. Although Spinach/Broccoli-DFHBI complexes exhibit high fluorescence in vitro, their fluorescenc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc5d6ab5777bf3aea08adf2307451de7
https://doi.org/10.1002/ange.201914576
https://doi.org/10.1002/ange.201914576
Autor:
Jacob L. Litke, Samie R. Jaffrey
Publikováno v:
Methods
Circular RNAs are useful entities for various biotechnology applications, such as templating translation and binding or sequestering miRNA and RNA binding proteins. Circular RNA as highly resistant to degradation in cells and are more long-lived than
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f314b96b6dcfd004e44d097306bd276
https://pubmed.ncbi.nlm.nih.gov/33992775
https://pubmed.ncbi.nlm.nih.gov/33992775
Publikováno v:
International Journal of Molecular Sciences, Vol 17, Iss 3, p 366 (2016)
Holliday Junctions are critical DNA intermediates central to double strand break repair and homologous recombination. The junctions can adopt two general forms: open and stacked-X, which are induced by protein or ion binding. In this work, fluorescen
Externí odkaz:
https://doaj.org/article/b1b52ba67ef047d59edc6496583f0569
Autor:
Sourav K. Dey, Xing Li, Jacob L. Litke, Jared D. Moon, Jiahui Wu, Hyaeyeong Kim, Samie R. Jaffrey
Publikováno v:
Cell Chem Biol
Summary Small molecules can be imaged in living cells using biosensors composed of RNA. However, RNA-based devices are difficult to design. Here, we describe a versatile platform for designing RNA-based fluorescent small-molecule sensors using natura
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0967faa3c973651571eee3db4fa13ed7
https://pubmed.ncbi.nlm.nih.gov/34010626
https://pubmed.ncbi.nlm.nih.gov/34010626
Publikováno v:
Cell chemical biology. 26(4)
Summary Genetically encoded biosensors are useful tools for detecting the presence and levels of diverse biomolecules in living cells. However, low-abundance targets are difficult to detect because they are often unable to bind and activate enough bi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b4df8f321061d76a10f39bb7e81c0ee9
https://pubmed.ncbi.nlm.nih.gov/31002799
https://pubmed.ncbi.nlm.nih.gov/31002799
Autor:
Samie R. Jaffrey, Jacob L. Litke
Publikováno v:
Nature biotechnology
RNA aptamers and RNA aptamer-based devices can be genetically encoded and expressed in cells to probe and manipulate cellular function. However, their usefulness in the mammalian cell is limited by low expression and rapid degradation. Here we descri
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::64b27bfb583a44497cf97e57d4b28e50
https://pubmed.ncbi.nlm.nih.gov/30962542
https://pubmed.ncbi.nlm.nih.gov/30962542
Developing Fluorogenic Riboswitches for Imaging Metabolite Concentration Dynamics in Bacterial Cells
Genetically encoded small-molecule sensors are important tools for revealing the dynamics of metabolites and other small molecules in live cells over time. We recently developed RNA-based sensors that exhibit fluorescence in proportion to a small-mol
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c5c53315febafb8f11c46ea1c2e036ee
https://europepmc.org/articles/PMC5540731/
https://europepmc.org/articles/PMC5540731/