Absence, loss-of-function, or inhibition of Escherichia coli AcrB does not increase expression of other efflux pump genes supporting the discovery of AcrB inhibitors as antibiotic adjuvants

Autor: Maria Laura Ciusa, Robert L Marshall, Vito Ricci, Jack W Stone, Laura J V Piddock

Publikováno v: Journal of Antimicrobial Chemotherapy. 77:633-640

Objectives To determine whether expression of efflux pumps and antibiotic susceptibility are altered in Escherichia coli in response to efflux inhibition. Methods The promoter regions of nine efflux pump genes (acrAB, acrD, acrEF, emrAB, macAB, cusCF

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0495c2424f54454062b6d864a197dd2c
https://doi.org/10.1093/jac/dkab452

Perturbed structural dynamics underlie inhibition and altered efflux of the multidrug resistance pump AcrB

Autor: Vito Ricci, Laura J. V. Piddock, Paula J. Booth, Albert Konijnenberg, Andy M. Lau, Heather E. Findlay, Eamonn Reading, Argyris Politis, Jack W Stone, Attilio Vittorio Vargiu, Chiara Fais, Zainab Ahdash, Giuliano Malloci, Paolo Ruggerone, Elizabeth M Grimsey, Xuan Wang-Kan

Publikováno v: Nature Communications
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
Reading, E, Ahdash, Z, Fais, C, Ricci, V, Kan, X W, Grimsey, E, Stone, J, Malloci, G, Lau, A, Findlay, H, Konijnenberg, A, Booth, P, Ruggerone, P, Vargiu, A V, Piddock, L JV & Politis, A 2020, ' Perturbed structural dynamics underlie inhibition and altered efflux of the multidrug resistance pump AcrB ', Nature Communications, vol. 11, no. 1, 5565 . https://doi.org/10.1038/s41467-020-19397-2

Resistance–nodulation–division efflux pumps play a key role in inherent and evolved multidrug resistance in bacteria. AcrB, a prototypical member of this protein family, extrudes a wide range of antimicrobial agents out of bacteria. Although high

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d771468cd39b4496fbb870f1eef1179
https://pubmed.ncbi.nlm.nih.gov/33149158

Overexpression of RamA, Which Regulates Production of the Multidrug Resistance Efflux Pump AcrAB-TolC, Increases Mutation Rate and Influences Drug Resistance Phenotype

Autor: Jack W Stone, Vito Ricci, Laura J. V. Piddock, Elizabeth M Grimsey, Natasha Weston

Publikováno v: Antimicrob Agents Chemother

In Enterobacteriales, the AcrAB-TolC efflux pump exports substrates, including antimicrobials, from the cell. Overexpression of AcrAB-TolC can occur after exposure to fluoroquinolones, leading to multidrug resistance. The expression of AcrAB-TolC in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::087f985210356adc41638728c5d7bd8c
https://europepmc.org/articles/PMC7179326/