Precise optical control of gene expression in C elegans using improved genetic code expansion and Cre recombinase

Autor: Lloyd Davis, Inja Radman, Angeliki Goutou, Ailish Tynan, Kieran Baxter, Zhiyan Xi, Jack M O'Shea, Jason W Chin, Sebastian Greiss

Publikováno v: eLife, Vol 10 (2021)

Synthetic strategies for optically controlling gene expression may enable the precise spatiotemporal control of genes in any combination of cells that cannot be targeted with specific promoters. We develop an improved genetic code expansion system in

Externí odkaz: https://doaj.org/article/9ed4db72b5494942b206e4f1f417c6b3

Generation of Photocaged Nanobodies for Intracellular Applications in an Animal Using Genetic Code Expansion and Computationally Guided Protein Engineering

Autor: Jack M. O'Shea, Angeliki Goutou, Jack Brydon, Cyrus R. Sethna, Christopher W. Wood, Sebastian Greiss

Publikováno v: Shea, J M O, Goutou, A, Brydon, J, Sethna, C R, Wood, C W & Greiss, S 2022, ' Generation of photocaged nanobodies for intracellular applications in an animal using genetic code expansion and computationally guided protein engineering** ', ChemBioChem, vol. 23, no. 16, e202200321 . https://doi.org/10.1002/cbic.202200321

Nanobodies are becoming increasingly popular as tools for manipulating and visualising proteins in vivo. The ability to control nanobody/antigen interactions using light could provide precise spatiotemporal control over protein function. We develop a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f421909a07c43421c21566c182f85955
https://pubmed.ncbi.nlm.nih.gov/35731601

Generation of photocaged nanobodies for in vivo applications using genetic code expansion and computationally guided protein engineering

Autor: Christopher W. Wood, Sebastian Greiss, Angeliki Goutou, Jack M. O’Shea, Cyrus Sethna

Publikováno v: bioRxiv

Nanobodies are becoming increasingly popular as tools for manipulating and visualising proteins in vivo. The ability to control nanobody/antigen interactions using light could provide precise spatiotemporal control over protein function. We develop a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::575263463ed5acb5324cf94e0d018200
https://doi.org/10.1101/2021.04.16.440193

Author response: Direct glia-to-neuron transdifferentiation gives rise to a pair of male-specific neurons that ensure nimble male mating

Autor: Sophie P.R. Gilbert, Carla Lloret-Fernández, Rachel C. Bonnington, David H. Hall, Byunghyuk Kim, Richard J. Poole, Steven J. Cook, Arantza Barrios, Jack M O'Shea, Scott W. Emmons, Laura Molina-García, David Elliott, Michele Sammut

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::37d1cce7f0bc89835b3bc96d00cb8f0a
https://doi.org/10.7554/elife.48361.sa2

A direct glia-to-neuron natural transdifferentiation ensures nimble sensory-motor coordination of male mating behaviour

Autor: Richard J. Poole, Steven J. Cook, Rachel C. Bonnington, David Elliott, Jack M O'Shea, Scott W. Emmons, Arantza Barrios, Byunghyuk Kim, Laura Molina-García, David H. Hall, Michele Sammut, Sophie P.R. Gilbert

SUMMARYThe coordinated execution of innate, stereotyped sexual behaviours, such as courtship and mating, requires sexually dimorphic sensory-motor circuits that are genetically specified during development (reviewed in [1-3]). Studies in the nematode

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::346afb6bbed51b79cecc5a14daff2083