Zobrazeno 1 - 10
of 66
pro vyhledávání: '"Jack E. Richman"'
Autor:
Megan D. Smith, Lambros J. Tassoulas, Troy A. Biernath, Jack E. Richman, Kelly G. Aukema, Lawrence P. Wackett
Publikováno v:
Computational and Structural Biotechnology Journal, Vol 19, Iss , Pp 3087-3096 (2021)
The OleA enzyme is distinct amongst thiolase enzymes in binding two long (≥C8) acyl chains into structurally-opposed hydrophobic channels, denoted A and B, to carry out a non-decarboxylative Claisen condensation reaction and initiate the biosynthes
Externí odkaz:
https://doaj.org/article/cc59355c65704fb1ad858e57870f7987
Publikováno v:
mBio, Vol 12, Iss 6 (2021)
ABSTRACT Perfluorinated carbon atoms in a diether linkage are common in commercial anesthetics, drugs, fungicides, and insecticides. An important chemical group comprising perfluorodiethers is the 2,2-fluoro-1,3-benzodioxole (DFBD) moiety. The fluori
Externí odkaz:
https://doaj.org/article/11fc1f1e861943819e2b5e7d3b9957f5
Publikováno v:
Environmental Microbiology. 24:5202-5216
The present study examined the regulatory and metabolic response of the aromatic degrader Pseudomonas putida F1 and its tod operon, controlling toluene degradation, to fluorinated aromatic and aliphatic compounds. The tod operon is upregulated by ind
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e59705c1327977cf7e89b81f35c8cab8
https://doi.org/10.1111/1462-2920.16187
https://doi.org/10.1111/1462-2920.16187
Autor:
Jack E. Richman, Lawrence P. Wackett, Kelly G. Aukema, Megan D. Smith, Lambros J. Tassoulas, Troy A. Biernath
Publikováno v:
Computational and Structural Biotechnology Journal, Vol 19, Iss, Pp 3087-3096 (2021)
Computational and Structural Biotechnology Journal
Computational and Structural Biotechnology Journal
Graphical abstract The thiolase enzyme OleA initiates the biosynthesis of β-lactone natural products and membrane hydrocarbons via acyl-Coenzyme A (CoA) substrates, but this study showed that p-nitrophenyl alkanoates can substitute, yielding new ins
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6881f359266ae9a56527a999b9b1406f
http://www.sciencedirect.com/science/article/pii/S2001037021002129
http://www.sciencedirect.com/science/article/pii/S2001037021002129
Publikováno v:
Applied and environmental microbiology. 88(9)
The capacity to defluorinate polyfluorinated organic compounds is a rare phenotype in microbes but is increasingly considered important for maintaining the environment. New discoveries will be greatly facilitated by the ability to screen many natural
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::f21d4fdbdac8a9aadad7e25117f56c72
https://pubmed.ncbi.nlm.nih.gov/35435713
https://pubmed.ncbi.nlm.nih.gov/35435713
Publikováno v:
Synthetic Biology. 5
Enzymes in the thiolase superfamily catalyze carbon–carbon bond formation for the biosynthesis of polyhydroxyalkanoate storage molecules, membrane lipids and bioactive secondary metabolites. Natural and engineered thiolases have applications in syn
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1a3bdd0e384aa19a83592efd2d8243b0
https://doi.org/10.1093/synbio/ysaa004
https://doi.org/10.1093/synbio/ysaa004
Autor:
Carrie M. Wilmot, Lawrence P. Wackett, Jack E. Richman, Matthew R. Jensen, Jennifer Y. Neufeld, James K. Christenson
Publikováno v:
Biochemistry. 56:348-351
The first β-lactone synthetase enzyme is reported, creating an unexpected link between the biosynthesis of olefinic hydrocarbons and highly functionalized natural products. The enzyme OleC, involved in the microbial biosynthesis of long-chain olefin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::82da22b80c86e998e7125491481ba7f0
https://doi.org/10.1021/acs.biochem.6b01199
https://doi.org/10.1021/acs.biochem.6b01199
Autor:
Serina L. Robinson, Lawrence P. Wackett, Dallas R. Fonseca, João Neres, James K. Christenson, Dominick J. Jenkins, Jack E. Richman, Courtney C. Aldrich
Publikováno v:
Chembiochem : a European journal of chemical biology. 20(13)
Enzyme-catalyzed β-lactone formation from β-hydroxy acids is a crucial step in bacterial biosynthesis of β-lactone natural products and membrane hydrocarbons. We developed a novel, continuous assay for β-lactone synthetase activity using syntheti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2e57e0153fa96aaf25a0043ecdb9b037
https://pubmed.ncbi.nlm.nih.gov/30856684
https://pubmed.ncbi.nlm.nih.gov/30856684
Autor:
Tiffany A. Engel, Lawrence P. Wackett, An N Kim, James K. Christenson, Serina L. Robinson, Jack E. Richman
Publikováno v:
Biochemistry. 56(40)
OleB is an α/β-hydrolase found in bacteria that biosynthesize long-chain olefinic hydrocarbons, but its function has remained obscure. We report that OleB from the Gram-negative bacterium Xanthomonas campestris performs an unprecedented β-lactone
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bdd26dc31ba5cc587d90a8b3ab42d2a7
https://pubmed.ncbi.nlm.nih.gov/28872321
https://pubmed.ncbi.nlm.nih.gov/28872321
Autor:
Sebastian A. Stoian, Jack E. Richman, Eckard Münck, Kelly G. Aukema, Lawrence P. Wackett, Thomas M. Makris, John D. Lipscomb
Publikováno v:
ACS Catalysis. 3:2228-2238
Aldehyde-deformylating oxygenase (ADO) catalyzes O2-dependent release of the terminal carbon of a biological substrate, octadecanal, to yield formate and heptadecane in a reaction that requires external reducing equivalents. We show here that ADO als
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1c7f3a5e5bf492c6850d78e3f5e64d30
https://doi.org/10.1021/cs400484m
https://doi.org/10.1021/cs400484m