Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Jack E. Bramham"'
Publikováno v:
Communications Chemistry, Vol 5, Iss 1, Pp 1-10 (2022)
In situ illumination of liquid-state NMR samples allows to characterise light-dependent chemical and biological phenomena, but, in practice, the position of an NMR sample deep within the bore of a spectrometer magnet renders such illumination challen
Externí odkaz:
https://doaj.org/article/150c3822e9a4412bbcd1200d0b823524
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-11 (2022)
Protein liquid-liquid phase separation is an important phenomenon in biology. Here, the authors demonstrate an approach to characterize the evolution of protein phases in both time and space using a fluorinated probe molecule in NMR spectroscopy.
Externí odkaz:
https://doaj.org/article/2d99dd63bce840cbbc11966991551f11
Publikováno v:
mAbs, Vol 13, Iss 1 (2021)
Subcutaneous injection of a low volume (100 mg/mL) formulation is an attractive administration strategy for monoclonal antibodies (mAbs) and other biopharmaceutical proteins. Using concentrated solutions may also be beneficial at various stages of bi
Externí odkaz:
https://doaj.org/article/afa5550877874a4fa2303dc1bee8e05b
Publikováno v:
Chemical communications (Cambridge, England). 58(85)
Photocaging is an attractive strategy to control molecular behaviour, for example, in chemical synthesis, interaction studies or photodynamic therapies. Here, we demonstrate that
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::9e7a636114bbf7eacda92a9b5b9006c8
https://pubmed.ncbi.nlm.nih.gov/36205575
https://pubmed.ncbi.nlm.nih.gov/36205575
Publikováno v:
Bramham, J E, Zalar, M & Golovanov, A P 2022, ' Controlled release and characterisation of photocaged molecules using in situ LED illumination in solution NMR spectroscopy ', Chemical Communications, vol. 58, no. 85, pp. 11973-11976 . https://doi.org/10.1039/D2CC04731D
Photocaging is an attractive strategy to control molecular behaviour, for example, in chemical synthesis, interaction studies or photodynamic therapies. Here, we demonstrate that in situ illumination by the LED NMRtorch approach enables effective and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::97ed70918ba91f415fdd4ad9e02510e9
https://uom.elsevierpure.com/en/publications/22a1060b-e7c4-48e7-a9b4-bea735267e7c
https://uom.elsevierpure.com/en/publications/22a1060b-e7c4-48e7-a9b4-bea735267e7c
Publikováno v:
Nature communications. 13(1)
Liquid-liquid phase separation (LLPS) of protein solutions is increasingly recognised as an important phenomenon in cell biology and biotechnology. However, opalescence and concentration fluctuations render LLPS difficult to study, particularly when
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::8525de511ab281428b165f08d499f548
https://pubmed.ncbi.nlm.nih.gov/35365630
https://pubmed.ncbi.nlm.nih.gov/35365630
Publikováno v:
mAbs
article-version (VoR) Version of Record
article-version (VoR) Version of Record
Subcutaneous injection of a low volume (100 mg/mL) formulation is an attractive administration strategy for monoclonal antibodies (mAbs) and other biopharmaceutical proteins. Using concentrated solutions may also be beneficial at various stages of bi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6b233384e434f42b688028b3f85fc8a7
https://pubmed.ncbi.nlm.nih.gov/34225583
https://pubmed.ncbi.nlm.nih.gov/34225583
Publikováno v:
Bramham, J E, Podmore, A, Davies, S A & Golovanov, A P 2021, ' Comprehensive Assessment of Protein and Excipient Stability in Biopharmaceutical Formulations Using H-1 NMR Spectroscopy ', ACS Pharmacology & Translational Science, vol. 4, no. 1, pp. 288-295 . https://doi.org/10.1021/acsptsci.0c00188
ACS Pharmacology & Translational Science
ACS Pharmacology & Translational Science
Biopharmaceutical proteins are important drug therapies in the treatment of a range of diseases. Proteins, such as antibodies (Abs) and peptides, are prone to chemical and physical degradation, particularly at the high concentrations currently sought
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ef7252eee46f4caaa50286e3985fa858
https://pure.manchester.ac.uk/ws/files/211514568/acsptsci.0c00188.pdf
https://pure.manchester.ac.uk/ws/files/211514568/acsptsci.0c00188.pdf
Autor:
Priscilla, Kheddo, Jack E, Bramham, Rebecca J, Dearman, Shahid, Uddin, Christopher F, van der Walle, Alexander P, Golovanov
Publikováno v:
Molecular pharmaceutics. 14(8)
Liquid-liquid phase separation (LLPS) of monoclonal antibody (mAb) formulations involves spontaneous separation into dense (protein-rich) and diluted (protein-lean) phases and should be avoided in the final drug product. Understanding the factors lea
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::19e6aee6f916e7297522969af9ef290b
https://pubmed.ncbi.nlm.nih.gov/28614662
https://pubmed.ncbi.nlm.nih.gov/28614662