Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Jaby Jacob"'
Publikováno v:
Journal of Pharmaceutical Sciences. 98:3013-3030
Two major aggregation pathways observed in an IgG2 molecule are described. Different aggregate species generated by long-term incubation of the antibody at 37°C were collected by a semi-preparative size exclusion chromatography method. These purifie
Autor:
Carl G. Kolvenbach, Arnold McAuley, David N. Brems, Stephen R. Brych, Jaby Jacob, Kimberly Westland, Masazumi Matsumura, Hyo Jin Lee, Gerd R. Kleemann, Douglas Rehder
Publikováno v:
Protein Science. 17:95-106
Recombinant human monoclonal antibodies have become important protein-based therapeutics for the treatment of various diseases. The antibody structure is complex, consisting of beta-sheet rich domains stabilized by multiple disulfide bridges. The dim
Autor:
Robin S. Dothager, Jaby Jacob, Ingo Ruczinski, Tobin R. Sosnick, Pappannan Thiyagarajan, Nikolina Cingel, Jonathan E. Kohn, Bojan Zagrovic, Sebastian Doniach, Vijay S. Pande, Thomas M. Dillon, Kevin W. Plaxco, Ian S. Millett, M. Zahid Hasan, Soenke Seifert
Publikováno v:
Proceedings of the National Academy of Sciences. 101:12491-12496
Spectroscopic studies have identified a number of proteins that appear to retain significant residual structure under even strongly denaturing conditions. Intrinsic viscosity, hydrodynamic radii, and small-angle x-ray scattering studies, in contrast,
Autor:
Thalia T. Mills, Sebastian Doniach, Gregory S. Maskel, Soenke Seifert, Daniel Herschlag, Simon G. J. Mochrie, Pappannan Thiyagarajan, Yu Bai, Rhiju Das, Lisa W. Kwok, Ian S. Millett, Jaby Jacob, Lois Pollack
Publikováno v:
Journal of Molecular Biology. 332:311-319
Large RNAs can collapse into compact conformations well before the stable formation of the tertiary contacts that define their final folds. This study identifies likely physical mechanisms driving these early compaction events in RNA folding. We have
Autor:
Pappannan Thiyagarajan, Barry D. Stein, Jaby Jacob, Jeffrey M. Zaleski, Brian J. Kraft, Josef W. Zwanziger, Matthew A. Anspach, Robert Hart, Peter DeSanto
Publikováno v:
Chemistry of Materials. 14:4422-4429
We present combined results of optical, scattering, and spectroscopic studies of (K2O)15(Nb2O5)15(TeO2)70 glass and glass ceramic and comment on possible mechanisms of the optical behavior in terms of existing theory. The glass ceramic is confirmed t
Autor:
Hicham Fenniri, Pappannan Thiyagarajan, Klaas Hallenga, Alexander E. Ribbe, Bo-Liang Deng, Jaby Jacob
Publikováno v:
Proceedings of the National Academy of Sciences. 99:6487-6492
Rosette nanotubes are a new class of organic nanotubes obtained through the hierarchical self-assembly of low molecular weight synthetic modules in water. Here we demonstrate that these materials can serve as scaffolds for the supramolecular synthesi
Publikováno v:
Journal of the American Chemical Society. 125:6391-6393
Fundamental questions about the relative arrangement of the beta-sheet arrays within amyloid fibrils remain central to both its structure and the mechanism of self-assembly. Recent computational analyses suggested that sheet-to-sheet lamination was l
Autor:
Timothy S. Harvey, Pavel V. Bondarenko, Ramil F. Latypov, David N. Brems, Dingjiang Liu, Gerd R. Kleemann, Andrei A. Raibekas, Jaby Jacob
Publikováno v:
Biochemistry. 48(46)
Conformational properties of the folded and unfolded ensembles of human interleukin-1 receptor antagonist (IL-1ra) are strongly denaturant-dependent as evidenced by high-resolution two-dimensional nuclear magnetic resonance (NMR), limited proteolysis
Publikováno v:
Analytical chemistry. 79(7)
We have developed a new method for identification and quantification of succinimide in proteins. The method utilizes 18O water to monitor succinimide hydrolysis. 18O-labeled isoaspartic acid and aspartic acid peptides were produced by hydrolysis of a
Publikováno v:
Journal of molecular biology. 367(3)
The dimensions of a denatured protein, fully reduced ribonuclease A (r-RNase A), have been measured using synchrotron-based small angle X-ray scattering. The radius of gyration, 34-35 A, is unchanged from 0-6 M guanidinium chloride and from 20-90 deg