Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Jaap Brink"'
Autor:
Man Wei Tam, Jaap Brink
Publikováno v:
Journal of Structural Biology. 116:144-149
We have developed two software packages for the processing of electron diffraction patterns acquired on a slow-scan CCD camera. Automated indexing and processing of these electron diffraction patterns is possible using a program called AUTO. To obtai
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::98a41f4686df370eee94b15c32da3fdd
https://doi.org/10.1006/jsbi.1996.0023
https://doi.org/10.1006/jsbi.1996.0023
Autor:
Jaap Brink, Wah Chiu
Publikováno v:
Journal of Structural Biology. 113:23-34
A Gatan 1024 × 1024 slow-scan charge-coupled device (CCD) camera has been interfaced to a JEOL4000EX electron cryomicroscope and explored for its usefulness in the electron crystallographic analysis of thin, glucose-embedded crystals of crotoxin com
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c6931b4ffb0d524995a867a887390e23
https://doi.org/10.1006/jsbi.1994.1029
https://doi.org/10.1006/jsbi.1994.1029
Publikováno v:
Ultramicroscopy. 52(2)
Electron energy loss spectroscopy and dark-field imaging in a scanning transmission electrton microscope were used to determine the thickness of glucose-embedded crotoxin complex crystals. The results demonstrate the feasibility of identifying protei
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e7f8c5d19666e6bc03a41dd3412d0820
https://pubmed.ncbi.nlm.nih.gov/8291165
https://pubmed.ncbi.nlm.nih.gov/8291165
Publikováno v:
Proceedings, annual meeting, Electron Microscopy Society of America. 53:8-9
In protein electron crystallography, high resolution amplitudes and phases are required for a 3D reconstruction of a protein in which one can trace the Cα-backbone. Recording of these amplitudes and phases has so far been done on photographic film.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::10a92f1bda0ad82626d964ecadb5c70c
https://doi.org/10.1017/s0424820100136416
https://doi.org/10.1017/s0424820100136416
Autor:
Jaap Brink, Wah Chiu
Publikováno v:
Proceedings, annual meeting, Electron Microscopy Society of America. 52:104-105
Crotoxin complex is the principal neurotoxin of the South American rattlesnake, Crotalus durissus terrificus and has a molecular weight of 24 kDa. The protein is a heterodimer with subunit A assigneda chaperone function. Subunit B carries the lethal
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ac06f4cd553b5ebc8c6754617d301167
https://doi.org/10.1017/s0424820100168256
https://doi.org/10.1017/s0424820100168256
Publikováno v:
Proceedings, annual meeting, Electron Microscopy Society of America. 52:98-99
In protein electron crystallography, both low dose electron diffraction patterns and images are needed to provide accurate amplitudes and phases respectively for a 3-dimensional reconstruction. We have demonstrated that the Gatan 1024x1024 model 679
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a183b4fdb4583dbea6a1a520baa22cb7
https://doi.org/10.1017/s0424820100168220
https://doi.org/10.1017/s0424820100168220
Publikováno v:
Proceedings, annual meeting, Electron Microscopy Society of America. 52:118-119
There is an ever present need in electron microscopy of biological specimens to improve the quality of the recorded data to approach the atomic resolution capability of the modem instruments. The method adopted which has yielded the only significant
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d6835c75f99ff841ce9099f3949d404d
https://doi.org/10.1017/s0424820100168323
https://doi.org/10.1017/s0424820100168323
Publikováno v:
Proceedings, annual meeting, Electron Microscopy Society of America. 51:686-687
In three-dimensional structure determination of macromolccules by electron crystallography it is necessary to combine diffraction patterns and images recorded at various tilt angles from different crystals. In order to merge these data sets successfu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::148e655b40fc5ddfca0d0ac9c3d6b86b
https://doi.org/10.1017/s042482010014926x
https://doi.org/10.1017/s042482010014926x
Publikováno v:
Proceedings, annual meeting, Electron Microscopy Society of America. 50:1054-1055
Protein electron crystallography can provide a reconstruction detailed enough for the polypeptide chain of a membrane protein to be traced (1). However, there remain a number of technical problems associated with this technique that must be overcome
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::634c8d5d8a5f1ffc3778bd75a14ff3d6
https://doi.org/10.1017/s0424820100129905
https://doi.org/10.1017/s0424820100129905
Publikováno v:
Ultramicroscopy. 27:91-99
Two-dimensional crystals of bovine NADH: ubiquinone oxidoreductase fragments were studied by cryo-electron microscopy and computer image analysis at two focus settings. The lattice parameters of the crystals were determined as a =14.5±0.2 nm , b =14
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c94781a0a1a60ccc78ce235a9a91c57e
https://doi.org/10.1016/0304-3991(89)90203-9
https://doi.org/10.1016/0304-3991(89)90203-9