Generation of an antibody against the protein phosphatase 1 inhibitor KEPI and characterization of the epitope

Autor: Katjana Daskalow, Boisguerin P, Jandrig B, Fk, Landeghem, Volkmer R, Micheel B, Ja, Schenk

Publikováno v: Scopus-Elsevier
Europe PubMed Central

A monoclonal antibody against the potential tumor suppressor kinase-enhanced protein phosphatase 1 (PP1) inhibitor KEPI (PPP1R14C) was generated and characterized. Human KEPI was expressed in Escherichia coli and used to immunize Balb/c mice. Using h

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::afba17a4d608c2a6773166de1156ce04
https://publishup.uni-potsdam.de/frontdoor/index/index/docId/32120

A broadly cross-reactive monoclonal antibody against hepatitis E virus capsid antigen.

Autor: Kubickova B; Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, 17493, Greifswald-Insel Riems, Germany.; RECETOX, Faculty of Science, Masaryk University, 62500, Brno, Czech Republic., Schenk JA; Hybrotec GmbH, 14476, Potsdam, Germany.; UP Transfer GmbH an der Universität Potsdam, 14469, Potsdam, Germany., Ramm F; Branch Bioanalytics and Bioprocesses (IZI-BB), Fraunhofer Institute for Cell Therapy and Immunology (IZI), 14476, Potsdam, Germany.; Institute of Chemistry and Biochemistry, Freie Universität Berlin, 14195, Berlin, Germany., Markuškienė K; Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, 17493, Greifswald-Insel Riems, Germany.; Institute of Biotechnology, Life Sciences Centre, Vilnius University, 02241, Vilnius, Lithuania., Reetz J; German Federal Institute for Risk Assessment, 10589, Berlin, Germany., Dremsek P; Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, 17493, Greifswald-Insel Riems, Germany.; Zentrum für Pathobiochemie und Genetik, Medizinische Universität Wien, 1090, Wien, Austria., Tamosiunas PL; Institute of Biotechnology, Life Sciences Centre, Vilnius University, 02241, Vilnius, Lithuania., Cepulyte L; Institute of Biotechnology, Life Sciences Centre, Vilnius University, 02241, Vilnius, Lithuania., Trinh HA; Branch Bioanalytics and Bioprocesses (IZI-BB), Fraunhofer Institute for Cell Therapy and Immunology (IZI), 14476, Potsdam, Germany., Scholz J; German Federal Institute for Risk Assessment, 10589, Berlin, Germany., Memczak H; qpa bioanalytics GmbH, 10585, Berlin, Germany., Hovestädt M; qpa bioanalytics GmbH, 10585, Berlin, Germany.; Surflay Nanotec, 12489, Berlin, Germany., Ryll R; Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, 17493, Greifswald-Insel Riems, Germany., Petraityte-Burneikiene R; Institute of Biotechnology, Life Sciences Centre, Vilnius University, 02241, Vilnius, Lithuania., Corman VM; Institute of Virology, Charité - Universitätsmedizin Berlin, 10117, Berlin, Germany.; Site Berlin, German Center for Infection Research (DZIF), 10117, Berlin, Germany., Andersson A; UP Transfer GmbH an der Universität Potsdam, 14469, Potsdam, Germany.; Branch Bioanalytics and Bioprocesses (IZI-BB), Fraunhofer Institute for Cell Therapy and Immunology (IZI), 14476, Potsdam, Germany., Becher D; Micromun GmbH, 17489, Greifswald, Germany., Groschup MH; Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, 17493, Greifswald-Insel Riems, Germany.; Partner site Hamburg-Lübeck-Borstel-Riems, German Center for Infection Research (DZIF), 17493, Greifswald-Insel Riems, Germany., Kubick S; Branch Bioanalytics and Bioprocesses (IZI-BB), Fraunhofer Institute for Cell Therapy and Immunology (IZI), 14476, Potsdam, Germany.; Institute of Chemistry and Biochemistry, Freie Universität Berlin, 14195, Berlin, Germany.; Faculty of Health Sciences, Joint Faculty of the Brandenburg University of Technology Cottbus-Senftenberg, the Brandenburg Medical School Theodor Fontane and the University of Potsdam, Potsdam, Germany., Sellrie F; Hybrotec GmbH, 14476, Potsdam, Germany.; UP Transfer GmbH an der Universität Potsdam, 14469, Potsdam, Germany., Johne R; German Federal Institute for Risk Assessment, 10589, Berlin, Germany., Ulrich RG; Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, 17493, Greifswald-Insel Riems, Germany. rainer.ulrich@fli.de.; Partner site Hamburg-Lübeck-Borstel-Riems, German Center for Infection Research (DZIF), 17493, Greifswald-Insel Riems, Germany. rainer.ulrich@fli.de.

Publikováno v: Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2021 Jun; Vol. 105 (12), pp. 4957-4973. Date of Electronic Publication: 2021 Jun 15.

Dye Tool Box for a Fluorescence Enhancement Immunoassay.

Autor: Eisold U; Institute of Chemistry, University of Potsdam , Karl-Liebknecht-Str. 24-25, 14476 Potsdam, Germany., Sellrie F; UP Transfer GmbH , Am Neuen Palais 10, 14469 Potsdam, Germany.; Hybrotec GmbH , Am Mühlenberg 11, 14476 Potsdam, Germany., Memczak H; qpa bioanalytics GmbH , 10409 Berlin, Germany., Andersson A; Hybrotec GmbH , Am Mühlenberg 11, 14476 Potsdam, Germany., Schenk JA; UP Transfer GmbH , Am Neuen Palais 10, 14469 Potsdam, Germany.; Hybrotec GmbH , Am Mühlenberg 11, 14476 Potsdam, Germany., Kumke MU; Institute of Chemistry, University of Potsdam , Karl-Liebknecht-Str. 24-25, 14476 Potsdam, Germany.

Publikováno v: Bioconjugate chemistry [Bioconjug Chem] 2018 Jan 17; Vol. 29 (1), pp. 203-214. Date of Electronic Publication: 2018 Jan 08.

Bright or dark immune complexes of anti-TAMRA antibodies for adapted fluorescence-based bioanalysis.

Autor: Eisold U; Department of Chemistry, Physical Chemistry, Universität Potsdam, Karl-Liebknecht-Str. 24-25, 14476, Potsdam, Germany., Sellrie F, Schenk JA, Lenz C, Stöcklein WF, Kumke MU

Publikováno v: Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2015 May; Vol. 407 (12), pp. 3313-23. Date of Electronic Publication: 2015 Feb 25.

Comparative characterization of mAb producing hapten-specific hybridoma cells by flow cytometric analysis and ELISA.

Autor: Kuhne M; BAM Federal Institute for Materials Research and Testing, Richard-Willstätter-Str. 11, D-12489 Berlin, Germany., Dippong M; BAM Federal Institute for Materials Research and Testing, Richard-Willstätter-Str. 11, D-12489 Berlin, Germany; University of Potsdam, Institute for Biochemistry and Biology, Karl-Liebknecht-Str. 24-25, 14476 Potsdam, Germany., Flemig S; BAM Federal Institute for Materials Research and Testing, Richard-Willstätter-Str. 11, D-12489 Berlin, Germany., Hoffmann K; BAM Federal Institute for Materials Research and Testing, Richard-Willstätter-Str. 11, D-12489 Berlin, Germany., Petsch K; BAM Federal Institute for Materials Research and Testing, Richard-Willstätter-Str. 11, D-12489 Berlin, Germany., Schenk JA; UP Transfer GmbH, Am Neuen Palais 10, D-14469 Potsdam, Germany; Hybrotec GmbH, Am Mühlenberg 11, D-14476 Potsdam-Golm, Germany., Kunte HJ; BAM Federal Institute for Materials Research and Testing, Richard-Willstätter-Str. 11, D-12489 Berlin, Germany., Schneider RJ; BAM Federal Institute for Materials Research and Testing, Richard-Willstätter-Str. 11, D-12489 Berlin, Germany. Electronic address: rudolf.schneider@bam.de.

Publikováno v: Journal of immunological methods [J Immunol Methods] 2014 Nov; Vol. 413, pp. 45-56. Date of Electronic Publication: 2014 Jul 21.