Zobrazeno 1 - 10
of 413
pro vyhledávání: '"JUN ISHII"'
Autor:
Ririka Asama, Cher J. S. Liu, Masahiro Tominaga, Yu-Ru Cheng, Yasuyuki Nakamura, Akihiko Kondo, Hsiang-Yu Wang, Jun Ishii
Publikováno v:
Microbial Cell Factories, Vol 23, Iss 1, Pp 1-11 (2024)
Abstract Background Single-cell droplet microfluidics is an important platform for high-throughput analyses and screening because it provides an independent and compartmentalized microenvironment for reaction or cultivation by coencapsulating individ
Externí odkaz:
https://doaj.org/article/d6f0c6668bd34b15a76309e425c41424
Publikováno v:
Microbial Cell Factories, Vol 22, Iss 1, Pp 1-15 (2023)
Abstract Background “ATP wasting” has been observed in 13C metabolic flux analyses of Saccharomyces cerevisiae, a yeast strain commonly used to produce ethanol. Some strains of S. cerevisiae, such as the sake strain Kyokai 7, consume approximatel
Externí odkaz:
https://doaj.org/article/3f86fb82051040f48e464361b3ac590f
Publikováno v:
Microbial Cell Factories, Vol 22, Iss 1, Pp 1-16 (2023)
Abstract Background The filamentous fungus Trichoderma reesei has been used as a host organism for the production of lignocellulosic biomass-degrading enzymes. Although this microorganism has high potential for protein production, it has not yet been
Externí odkaz:
https://doaj.org/article/71efaaa31b2c4cbaa08c2238944da0c5
Autor:
Yoichiro Ito, Misa Ishigami, Noriko Hashiba, Yasuyuki Nakamura, Goro Terai, Tomohisa Hasunuma, Jun Ishii, Akihiko Kondo
Publikováno v:
Microbial Biotechnology, Vol 15, Iss 9, Pp 2364-2378 (2022)
Summary In our previous study, we serendipitously discovered that protein secretion in the methylotrophic yeast Pichia pastoris is enhanced by a mutation (V50A) in the mating factor alpha (MFα) prepro‐leader signal derived from Saccharomyces cerev
Externí odkaz:
https://doaj.org/article/9ab13543e8bf4a56b68f62305629a389
Autor:
Yoichiro Ito, Misa Ishigami, Goro Terai, Yasuyuki Nakamura, Noriko Hashiba, Teruyuki Nishi, Hikaru Nakazawa, Tomohisa Hasunuma, Kiyoshi Asai, Mitsuo Umetsu, Jun Ishii, Akihiko Kondo
Publikováno v:
Communications Biology, Vol 5, Iss 1, Pp 1-12 (2022)
A high-throughput workflow involving a streamlined genome-wide screen improves protein production in the non-conventional yeast, Komagataella phaffii, following adaptive laboratory evolution.
Externí odkaz:
https://doaj.org/article/f8d6b4a76d204cf09f7a566450ea6f5c
Autor:
Christopher J. Vavricka, Shunsuke Takahashi, Naoki Watanabe, Musashi Takenaka, Mami Matsuda, Takanobu Yoshida, Ryo Suzuki, Hiromasa Kiyota, Jianyong Li, Hiromichi Minami, Jun Ishii, Kenji Tsuge, Michihiro Araki, Akihiko Kondo, Tomohisa Hasunuma
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-14 (2022)
Producing plant secondary metabolites by microbes is limited by the known enzymatic reactions. Here, the authors apply machine learning to predict missing link enzymes of benzylisoquinoline alkaloid (BIA) biosynthesis in Papaver somniferum, and valid
Externí odkaz:
https://doaj.org/article/baf664b334934b1d89704e14d973d831
Autor:
Masahiko Sugimura, Taisuke Seike, Nobuyuki Okahashi, Yoshihiro Izumi, Takeshi Bamba, Jun Ishii, Fumio Matsuda
Publikováno v:
International Journal of Molecular Sciences, Vol 24, Iss 22, p 16378 (2023)
Saccharomyces cerevisiae is a promising host for the bioproduction of higher alcohols, such as 2,3-butanediol (2,3-BDO). Metabolically engineered S. cerevisiae strains that produce 2,3-BDO via glycolysis have been constructed. However, the specific 2
Externí odkaz:
https://doaj.org/article/0bace5781b90425084df2e3e5a26227c
Autor:
Erika Yoshida, Motoki Kojima, Munenori Suzuki, Fumio Matsuda, Kazutaka Shimbo, Akiko Onuki, Yousuke Nishio, Yoshihiro Usuda, Akihiko Kondo, Jun Ishii
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
Abstract (−)-Carvone is a monoterpenoid with a spearmint flavor. A sustainable biotechnological production process for (−)-carvone is desirable. Although all enzymes in (−)-carvone biosynthesis have been functionally expressed in Escherichia co
Externí odkaz:
https://doaj.org/article/5e010c658d71455cb769685da713f645
Publikováno v:
Frontiers in Chemical Engineering, Vol 4 (2022)
Flavonoids, a major group of secondary metabolites in plants, are promising for use as pharmaceuticals and food supplements due to their health-promoting biological activities. Industrial flavonoid production primarily depends on isolation from plant
Externí odkaz:
https://doaj.org/article/29e4c58e5a7d4f909ff303e22ca05546
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
Eukaryotic genetic switches are more complex than prokaryotic ones, complicating their design. Here the authors present a workflow for parallel screening, selection and evolution of yeast genetic switches.
Externí odkaz:
https://doaj.org/article/3522a0a8de284a4c85646d76fb5876fa