Zobrazeno 1 - 10
of 256
pro vyhledávání: '"JOSEPH SHILOACH"'
Autor:
Yan Li, Xizhong Cui, Joseph Shiloach, Jeffrey Wang, Dante A. Suffredini, Wanying Xu, Wancang Liu, Yvonne Fitz, Junfeng Sun, Peter Q. Eichacker
Publikováno v:
Intensive Care Medicine Experimental, Vol 8, Iss 1, Pp 1-15 (2020)
Abstract Background Lethal B. anthracis infection produces high proinflammatory peptidoglycan (PGN) burdens in hosts. We investigated whether the lethality and inflammation anthrax PGN can produce are related. Methods At 6 h before and the start of 2
Externí odkaz:
https://doaj.org/article/989b67f6177540a7943a2679261a6b8a
Publikováno v:
Microbial Cell Factories, Vol 19, Iss 1, Pp 1-10 (2020)
Abstract Background The expression of recombinant proteins triggers a stress response which downregulates key metabolic pathway genes leading to a decline in cellular health and feedback inhibition of both growth and protein expression. Instead of in
Externí odkaz:
https://doaj.org/article/a1f05ed09dea43d4a478a6b7d1cae058
Publikováno v:
Microbial Biotechnology, Vol 12, Iss 6, Pp 1226-1236 (2019)
Summary Pichia pastoris KM71H (MutS) is an efficient producer of hard‐to‐express proteins such as the membrane protein P‐glycoprotein (Pgp), an ATP‐powered efflux pump which is expressed properly, but at very low concentration, using the conv
Externí odkaz:
https://doaj.org/article/d54b4b6ab4c74d93ab7c38ccec71f4c1
Autor:
Raghuveer Kavarthapu, Rajakumar Anbazhagan, Ashish K. Sharma, Joseph Shiloach, Maria L. Dufau
Publikováno v:
Frontiers in Cell and Developmental Biology, Vol 8 (2020)
GRTH/DDX25 is a testicular RNA helicase expressed in germ cells that plays a crucial role in completion of spermatogenesis. Previously, we demonstrated a missense mutation (R242H) of GRTH gene in Japanese infertile patients (5.8%) with non-obstructiv
Externí odkaz:
https://doaj.org/article/4a72f074f48341d996e0f34d728d6278
Autor:
Alejandro Negrete, Joseph Shiloach
Publikováno v:
Microbial Cell Factories, Vol 16, Iss 1, Pp 1-12 (2017)
Abstract Efficient growth of E. coli, especially for production of recombinant proteins, has been a challenge for the biotechnological industry since the early 1970s. By employing multiple approaches, such as different media composition, various grow
Externí odkaz:
https://doaj.org/article/aeeb76f6596f4ff6a5137f62b2d041fc
Publikováno v:
Cells, Vol 10, Iss 7, p 1667 (2021)
The HEK293 cell line has earned its place as a producer of biotherapeutics. In addition to its ease of growth in serum-free suspension culture and its amenability to transfection, this cell line’s most important attribute is its human origin, which
Externí odkaz:
https://doaj.org/article/f38029b7e32f45b1bcb5c31d0b5d935c
Autor:
Tanya Tschirhart, Eunkyoung Kim, Ryan McKay, Hana Ueda, Hsuan-Chen Wu, Alex Eli Pottash, Amin Zargar, Alejandro Negrete, Joseph Shiloach, Gregory F. Payne, William E. Bentley
Publikováno v:
Nature Communications, Vol 8, Iss 1, Pp 1-11 (2017)
Synthetic biology offers the ability to explore new ways of manipulating gene expression and function. Here the authors demonstrate an electrogenetic device that allows control of transcription by an exogenous electrical signal.
Externí odkaz:
https://doaj.org/article/0dbb255cb7794f2994f754cbab9bd21f
Autor:
Artur Muszyński, Kol A. Zarember, Christian Heiss, Joseph Shiloach, Lars J. Berg, John Audley, Arina Kozyr, David E. Greenberg, Steven M. Holland, Harry L. Malech, Parastoo Azadi, Russell W. Carlson, John I. Gallin
Publikováno v:
International Journal of Molecular Sciences, Vol 22, Iss 7, p 3303 (2021)
Granulibacter bethesdensis can infect patients with chronic granulomatous disease, an immunodeficiency caused by reduced phagocyte NADPH oxidase function. Intact G. bethesdensis (Gb) is hypostimulatory compared to Escherichia coli, i.e., cytokine pro
Externí odkaz:
https://doaj.org/article/7742a9e28acd472eb3687d321e9e745a
Publikováno v:
PLoS ONE, Vol 11, Iss 2, p e0148075 (2016)
The epithelial-mesenchymal transition (EMT) is a complex cancer progression that can boost the metastatic potential of transformed cells by inducing migration, loss of cell adhesion, and promoting proliferation under anchorage-independent conditions.
Externí odkaz:
https://doaj.org/article/9d1cc14b35a540d8bbf209ea3084c566
Autor:
Laura Abaandou, Joseph Shiloach
Publikováno v:
Medical Sciences, Vol 6, Iss 2, p 48 (2018)
Creating efficient cell lines is a priority for the biopharmaceutical industry, which produces biologicals for various uses. A recent approach to achieving this goal is the use of non-coding RNAs, microRNA (miRNA) and small interfering RNA (siRNA), t
Externí odkaz:
https://doaj.org/article/3963d3afea244917ae055378d4b02381