Zobrazeno 1 - 10
of 21
pro vyhledávání: '"JEAN-FRANÇOIS P. HAMEL"'
Autor:
Taehong Kwon, Holly Prentice, Jonas De Oliveira, Nyasha Madziva, Majid Ebrahimi Warkiani, Jean-François P. Hamel, Jongyoon Han
Publikováno v:
Scientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
Abstract Continuous production of biologics, a growing trend in the biopharmaceutical industry, requires a reliable and efficient cell retention device that also maintains cell viability. Current filtration methods, such as tangential flow filtration
Externí odkaz:
https://doaj.org/article/f3245607a2aa4041aa73c8e026a442cd
Publikováno v:
Analytical Chemistry
We demonstrate a new micro/nanofluidic system for continuous and automatic monitoring of protein product size and quantity directly from the culture supernatant during a high-cell-concentration CHO cell perfusion culture. A microfluidic device enable
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01a97e4a6bd941aa5bef43452d7317ca
http://europepmc.org/articles/PMC7309224
http://europepmc.org/articles/PMC7309224
Autor:
Jongyoon Han, Menglin Shang, Jean-François P. Hamel, Chwee Teck Lim, Taehong Kwon, Bee Luan Khoo
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
Scientific Reports
Scientific Reports
Chinese hamster ovary (CHO) cells have been the most commonly used mammalian host for large-scale commercial production of therapeutic proteins, such as monoclonal antibodies. Enhancement of productivity of these CHO cells is one of the top prioritie
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5305b957c6b83335213d410fed9076f5
https://doi.org/10.1038/s41598-020-80576-8
https://doi.org/10.1038/s41598-020-80576-8
Publikováno v:
Lab on a Chip. 18:2826-2837
Removing nonviable cells from a cell suspension is crucial in biotechnology and biomanufacturing. Label-free microfluidic cell separation devices based on dielectrophoresis, acoustophoresis, and deterministic lateral displacement are used to remove n
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::46eda1f3adf2052e471d3a9c66c8aba5
https://doi.org/10.1039/c8lc00250a
https://doi.org/10.1039/c8lc00250a
Autor:
Jongyoon Han, Jonas De Oliveira, Majid Ebrahimi Warkiani, Jean-François P. Hamel, Holly Prentice, Nyasha Madziva, Taehong Kwon
Publikováno v:
Scientific Reports
Scientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
Scientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
Continuous production of biologics, a growing trend in the biopharmaceutical industry, requires a reliable and efficient cell retention device that also maintains cell viability. Current filtration methods, such as tangential flow filtration using ho
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f411ebb9562afc4d19deff230c37fc86
http://europepmc.org/articles/PMC5532224
http://europepmc.org/articles/PMC5532224
Publikováno v:
Bioprocess and Biosystems Engineering. 32:267-276
Heat-shock protein, glycoprotein 96 (gp96), elicits both innate and adaptive immune responses against tumors or viral infections. In our laboratory, MethA tumor cell suspension culture process has been recently developed for gp96 production in spinne
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::85b93c2e30d54570a77c39cf5bda5d18
https://doi.org/10.1007/s00449-008-0247-z
https://doi.org/10.1007/s00449-008-0247-z
Autor:
Janet J. Hsu, Jean-François P. Hamel, Yulin Peng, Scott Lew, Wenglong R. Lin, Arnold L. Demain, Claudia C. Lee
Publikováno v:
Tetrahedron. 54:15915-15925
Acetate kinase (EC 2.7.2.1), an enzyme involved in the wasteful production of acetate during conversion of cellulose to ethanol by Clostridium thermocellum , was purified 144-fold. The enzyme has an M r of 84 kD by non-denaturing gradient gel electro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c4157f70008c7a2460583f136ab76725
https://doi.org/10.1016/s0040-4020(98)01001-1
https://doi.org/10.1016/s0040-4020(98)01001-1
Publikováno v:
Bioprocess and biosystems engineering. 32(4)
Heat-shock protein glycoprotein (gp96) serves as a natural adjuvant for chaperoning antigenic peptide into the immune surveillance pathway. In our laboratory, MethA tumor cell suspension culture process has been recently developed for gp96 production
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::34aed2c5bf31614ceb9f194f6fe01e65
https://pubmed.ncbi.nlm.nih.gov/18941797
https://pubmed.ncbi.nlm.nih.gov/18941797
Publikováno v:
Biotechnology progress. 23(6)
Heat-shock proteins (HSPs) act like "chaperones", making sure that the cell's proteins are in the right shape and in the right place at the right time. Heat-shock protein glycoprotein 96 (gp96) is a member of the HSP90 protein family, which chaperone
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae33b823b392e8322518ffe9f6a1a52f
https://pubmed.ncbi.nlm.nih.gov/17944484
https://pubmed.ncbi.nlm.nih.gov/17944484
Publikováno v:
Biotechnology progress. 23(1)
A novel wave bioreactor-perfusion culture system was developed for highly efficient production of monoclonal antibody IgG2a (mAb) by hybridoma cells. The system consists of a wave bioreactor, a floating membrane cell-retention filter, and a weight-ba
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2fe2ec91599d894b7dd2248fd59cebc8
https://pubmed.ncbi.nlm.nih.gov/17269696
https://pubmed.ncbi.nlm.nih.gov/17269696