Zobrazeno 1 - 10
of 27
pro vyhledávání: '"JAMES J. STEFFENS"'
Publikováno v:
Biochemistry. 39:8593-8602
We explore the use of site-directed mutations of scytalone dehydratase to study inhibitor binding interactions. The enzyme is the physiological target of new fungicides and the subject of inhibitor design and optimization. X-ray structures show that
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e094e4ffb9f99b0e35a65572227f11ef
https://doi.org/10.1021/bi000467m
https://doi.org/10.1021/bi000467m
Autor:
Tomas Lundqvist, Douglas B. Jordan, Ylva Lindqvist, James J. Steffens, Tatyana Sandalova, Gregory S. Basarab, Zdzislaw Wawrzak
Publikováno v:
Proteins: Structure, Function, and Genetics. 35:425-439
Scytalone dehydratase is a molecular target of inhibitor design efforts aimed at preventing the fungal disease caused by Magnaporthe grisea. A method for cocrystallization of enzyme with inhibitors at neutral pH has produced several crystal structure
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::263b9e735dfaf1abd02a21fd328e6541
https://doi.org/10.1002/(sici)1097-0134(19990601)35:4<425::aid-prot6>3.0.co
https://doi.org/10.1002/(sici)1097-0134(19990601)35:4<425::aid-prot6>3.0.co
Autor:
James J. Steffens, Beverly R Pfrogner, Tomas Lundqvist, Gregory S. Basarab, Rand S. Schwartz, Zdzislaw Wawrzak, Douglas B. Jordan
Publikováno v:
Pesticide Science. 55:277-280
The catalytic mechanism of scytalone dehydratase was examined by studying alternative substrates and site-directed mutations of active-site residues. Searches for an enol intermediate by looking for a half-reaction with authentic scytalone and 3,4-di
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1cd408375aeade9783d1c8570c02324b
https://doi.org/10.1002/(sici)1096-9063(199903)55:3<277::aid-ps893>3.0.co
https://doi.org/10.1002/(sici)1096-9063(199903)55:3<277::aid-ps893>3.0.co
Publikováno v:
Current Opinion in Biotechnology. 7:348-355
The disciplines traditionally used to investigate the mode of action of fungicides have been biochemistry and physiology. Over the past decade, classical and molecular genetics have been brought to bear on this problem with increasing success. Recent
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e4f66db4aab5eca6da48ec2bc0ffff35
https://doi.org/10.1016/s0958-1669(96)80043-7
https://doi.org/10.1016/s0958-1669(96)80043-7
Publikováno v:
Journal of Agricultural and Food Chemistry. 40:318-321
This is the first paper on the mode of action of a new class of broadly active foliar herbicides, 2-methylcinnoliniums. One of the most active compounds of this class is 2-methylcinnolinium-4-(o-methylphosphonate) (MCP). The symptoms of phytotoxicity
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d0123ace2087e46a797787b50c9db873
https://doi.org/10.1021/jf00014a030
https://doi.org/10.1021/jf00014a030
Publikováno v:
Structure (London, England : 1993). 8(10)
Background: The biosynthesis of the essential amino acid lysine in higher fungi and cyanobacteria occurs via the α-aminoadipate pathway, which is completely different from the lysine biosynthetic pathway found in plants and bacteria. The penultimate
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f50424350915020973629f7d0747b724
https://pubmed.ncbi.nlm.nih.gov/11080625
https://pubmed.ncbi.nlm.nih.gov/11080625
Publikováno v:
Acta crystallographica. Section D, Biological crystallography. 56(Pt 5)
The gene coding for saccharopine reductase (E.C. 1.5.1.10), an enzyme of the alpha-aminoadipic pathway of lysine biosynthesis in the pathogenic fungus Magnaporthe grisea, was cloned and expressed in Escherichia coli. The purified enzyme was crystalli
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01e9357fe75fa85797c43587f7ab57f8
https://pubmed.ncbi.nlm.nih.gov/10771443
https://pubmed.ncbi.nlm.nih.gov/10771443
Autor:
Gregory S. Basarab, Zdzislaw Wawrzak, Tomas Lundqvist, James J. Steffens, Douglas B. Jordan, Rand S. Schwartz
Publikováno v:
Biochemistry. 38(19)
On the basis of the X-ray crystal structure of scytalone dehydratase complexed with an active center inhibitor [Lundqvist, T., Rice, J., Hodge, C. N., Basarab, G. S., Pierce, J. and Lindqvist, Y. (1994) Structure (London) 2, 937-944], eight active-si
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dab243c571b69d69e37c73e8ee4a5f1a
https://pubmed.ncbi.nlm.nih.gov/10320327
https://pubmed.ncbi.nlm.nih.gov/10320327
Publikováno v:
Synthesis and Chemistry of Agrochemicals III ISBN: 9780841224735
Synthesis and Chemistry of Agrochemicals III
Synthesis and Chemistry of Agrochemicals III
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3e4d1953bc5df1fefc8fc59183982fd0
https://doi.org/10.1021/bk-1992-0504.ch001
https://doi.org/10.1021/bk-1992-0504.ch001
Publikováno v:
ACS Symposium Series ISBN: 9780841224735
Synthesis and Chemistry of Agrochemicals III
Synthesis and Chemistry of Agrochemicals III
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::87433302e895fa1188a220ad7146e76a
https://doi.org/10.1021/bk-1992-0504
https://doi.org/10.1021/bk-1992-0504