Zobrazeno 1 - 10
of 38
pro vyhledávání: '"J.W.D. GrootWassink"'
Publikováno v:
Scopus-Elsevier
Preparing homogeneous UDP-glucose:thiohydroximate S-glucosyltransferase (S-GT), the penultimate biosynthetic enzyme of glucosinolates, by standard chromatographic methods has yielded too little protein for adequate purity evaluation, identity verific
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::db318fa5c942520c543d60c5f3758d82
https://doi.org/10.1104/pp.105.1.425
https://doi.org/10.1104/pp.105.1.425
Publikováno v:
Journal of Plant Physiology. 136:356-361
Summary The last two steps in glucosinolate biosynthesis involve successively a glucosylation and a sulfation reaction, catalyzed by UDPglucose: thiohydroximate glucosyltransferase and PAPS: desulfoglucosinolate sulfotransferase. In an effort to obta
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::94262c213d4f814e937033c2652aff5a
https://doi.org/10.1016/s0176-1617(11)80062-3
https://doi.org/10.1016/s0176-1617(11)80062-3
Publikováno v:
Phytochemistry. 29:1425-1428
The enzyme 3′-phosphoadenosine-5′-phosphosulfate:desulphoglucosinolate sulphotransferase was partially purified from Brassica juncea cell cultures by fractional precipitation with ammonium sulphate, anion-exchange chromatography and gel filtratio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ac6319354e5f5ae4af99a66501bee69
https://doi.org/10.1016/0031-9422(90)80094-w
https://doi.org/10.1016/0031-9422(90)80094-w
Publikováno v:
Plant Science. 66:11-20
Feeding of 2-nitrobenzaldoxime to Brassica species led to the accumulation of the artificial 2-nitrophenylglucosinolate as well as several unknown sulfated side products. Isolation, proton nuclear magnetic resonance spectroscopy and hydrolysis by sul
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d5b78ddc915da70c9c65e33074a405cc
https://doi.org/10.1016/0168-9452(90)90163-i
https://doi.org/10.1016/0168-9452(90)90163-i
Publikováno v:
Progress in Plant Cellular and Molecular Biology ISBN: 9789401074452
Basic glucosinolates (GS) are synthesized from amino acids in two phases: 1. carbon chain extension and 2. main group formation, comprising N-oxidation, decarboxylation, sulfur incorporation, glucosylation and sulfation (Fig. 1). Conventional breedin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::869f129659fee50c7055d346b47f1180
https://doi.org/10.1007/978-94-009-2103-0_100
https://doi.org/10.1007/978-94-009-2103-0_100
Autor:
E.W.T. Tsang, J.W.D. GrootWassink
Publikováno v:
Enzyme and Microbial Technology. 10:297-301
The maintenance of hyperproduction of an exo-inulase (β-d-fructofuranoside fructohydrolase, EC 3.2.1.26) by a mutant of the yeast Kluyveromyces fragilis was examined in continuous culture on lactose. After initial phenotypic adaptation with activiti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::89a7527fd90865400192cea59dadc6f4
https://doi.org/10.1016/0141-0229(88)90131-7
https://doi.org/10.1016/0141-0229(88)90131-7
Autor:
G.M. Hewitt, J.W.D. GrootWassink
Publikováno v:
Enzyme and Microbial Technology. 6:263-270
Under different induction conditions, the industrial yeast Kluyveromyces fragilis is an excellent producer of the enzymes inulase (β- d -fructofuranoside fructohydrolase, EC 3.2.1.26) and lactase (β- d -galactoside galactohydrolase, EC 3.2.1.23), p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6468664962b3740220d960a79087bf10
https://doi.org/10.1016/0141-0229(84)90128-5
https://doi.org/10.1016/0141-0229(84)90128-5
Autor:
J.W.D. GrootWassink, S.E. Fleming
Publikováno v:
Enzyme and Microbial Technology. 2:45-53
The yeast Kluyveromyces fragilis was grown in continuous culture on a complex medium containing sucrose as the carbon source and limiting nutrient. The inulase yield was 7000 μg hexose/min.mg biomass, determined with 4% sucrose at 50°C and pH 5.0.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::704542861f759e1a563852ec0ac6d008
https://doi.org/10.1016/0141-0229(80)90007-1
https://doi.org/10.1016/0141-0229(80)90007-1
Publikováno v:
Biochimica et Biophysica Acta (BBA) - General Subjects. 926:119-126
Three forms of inulase were purified from Aspergillus niger by ultrafiltration, DEAE-Trisacryl chromatography Sephadex G-150 gel filtration, and preparative isoelectric focusing. The isoelectric focusing resolved inulase into three peaks with isoelec
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4d1788a6b557f9de37048ea9275526f4
https://doi.org/10.1016/0304-4165(87)90227-3
https://doi.org/10.1016/0304-4165(87)90227-3
Autor:
K.S. Lam, J.W.D. GrootWassink
Publikováno v:
Enzyme and Microbial Technology. 7:239-242
A simple and rapid procedure has been developed for the large-scale extraction of an exo-inulase (β- d -fructofuranoside fructohydrolase, EC 3.2.1.26) from the cell wall of a hyperproducing Kluyveromyces fragilis yeast strain. It involves resuspendi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d6423173f3fbcca49fd5be53601e2209
https://doi.org/10.1016/s0141-0229(85)80010-7
https://doi.org/10.1016/s0141-0229(85)80010-7