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Autor:
E. Fernández-Giménez, José Luis Vilas, Javier Vargas, Gabriel Caffarena, Carlos Oscar S. Sorzano, V. Peredo-Robinson, L. del Cano, Roberto Marabini, Joaquín Otón, Remedios Melero, Josué Gómez-Blanco, Pablo Conesa, Tomáš Majtner, J. Cuenca, J.M. de la Rosa-Trevín, José María Carazo
Publikováno v:
Journal of Structural Biology. 203:90-93
The introduction of Direct Electron Detector (DED) videos in the Electron Microscope field has boosted Single Particle Analysis to a point in which it is currently considered to be a key technique in Structural Biology. In this article we introduce a
Autor:
Roberto Melero, J. Navas, José María Carazo, Carlos Oscar S. Sorzano, Ilaria Peschiera, Ilaria Ferlenghi, Javier Vargas, Josué Gómez-Blanco, J.M. de la Rosa-Trevín, Roberto Marabini, José Luis Vilas, J. Cuenca
Publikováno v:
Journal of Structural Biology. 196:525-533
Random conical tilt (RCT) and orthogonal tilt reconstruction (OTR) are two remarkable methods for reconstructing the three-dimensional structure of macromolecules at low resolution. These techniques use two images at two different sample tilts. One o
Autor:
Grigory Sharov, Joaquín Otón, J. Gutiérrez, Javier Vargas, Carlos Oscar S. Sorzano, L. del Cano, A. Quintana, Jordi Burguet-Castell, A. Zaldívar, Mohsen Kazemi, J. Navas, Vahid Abrishami, Roberto Marabini, José María Carazo, I. Foche, J. Cuenca-Alba, José Luis Vilas, J.M. de la Rosa-Trevín, P. Conesa, Josué Gómez-Blanco
Publikováno v:
Journal of Structural Biology. 195:93-99
In the past few years, 3D electron microscopy (3DEM) has undergone a revolution in instrumentation and methodology. One of the central players in this wide-reaching change is the continuous development of image processing software. Here we present Sc
Autor:
Javier Vargas, A. Jimenez, María Jesús Martínez, Roberto Marabini, Carlos Oscar S. Sorzano, Pablo Conesa, José María Carazo, Erney Ramírez-Aportela, J.M. de la Rosa-Trevín, José Luis Vilas, Remedios Melero, David Maluenda
Publikováno v:
Journal of structural biology. 204(2)
The Map Challenge organized by the Electron Microscopy Data Bank has prompted the development of an Xmipp high resolution reconstruction protocol (which we will refer to as highres) that is integrated in the software platform Scipion . In this work w
Autor:
A. Jimenez, Erney Ramírez-Aportela, Y. Rancel, Jordi Mota, K. Savage, David Maluenda, José Luis Vilas, Carlos Oscar S. Sorzano, J.M. de la Rosa-Trevín, Marta Carroni, Roberto Marabini, Tomáš Majtner, Erik Lindahl, José María Carazo, L. del Cano, María Jesús Martínez, Remedios Melero, Pablo Conesa, Mark Basham, Alun W. Ashton, Stefan Fleischmann, Josué Gómez-Blanco, C. A. Siebert, Daniel K. Clare, Grigory Sharov
Publikováno v:
Digital.CSIC. Repositorio Institucional del CSIC
instname
Journal of Structural Biology
instname
Journal of Structural Biology
Three dimensional electron microscopy is becoming a very data-intensive field in which vast amounts of experimental images are acquired at high speed. To manage such large-scale projects, we had previously developed a modular workflow system called S
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::98e1b009d3f68211951bdab4db3579df
http://hdl.handle.net/10261/172712
http://hdl.handle.net/10261/172712
Autor:
J. Cuenca, Roberto Marabini, Pablo Conesa, Roberto Melero, José Luis Vilas, L. del Cano, Josué Gómez-Blanco, Javier Vargas, José María Carazo, J.M. de la Rosa-Trevín, Mohsen Kazemi, Joaquín Otón, Carlos Oscar S. Sorzano
Publikováno v:
BioMed Research International, Vol 2017 (2017)
BioMed Research International
BioMed Research International
One of the key steps in Electron Microscopy is the tomographic reconstruction of a three-dimensional (3D) map of the specimen being studied from a set of two-dimensional (2D) projections acquired at the microscope. This tomographic reconstruction may
Autor:
Carlos Oscar S. Sorzano, A. Zaldivar, Joaquín Otón, J.M. de la Rosa-Trevín, Roberto Marabini, Javier Vargas, José María Carazo
Publikováno v:
Journal of Structural Biology. 184:321-328
Xmipp is a specialized software package for image processing in electron microscopy, and that is mainly focused on 3D reconstruction of macromolecules through single-particles analysis. In this article we present Xmipp 3.0, a major release which intr
Autor:
Vahid Abrishami, A. Zaldivar, J.M. de la Rosa-Trevín, Roberto Marabini, Javier Vargas, José María Carazo, Carlos Oscar S. Sorzano
Publikováno v:
Journal of Structural Biology. 183:342-353
Three-dimensional reconstruction of biological specimens using electron microscopy by single particle methodologies requires the identification and extraction of the imaged particles from the acquired micrographs. Automatic and semiautomatic particle
Autor:
R. Marabini, Yoel Shkolnisky, A. Zaldívar-Peraza, Carlos Oscar S. Sorzano, José María Carazo, Javier Vargas, J.M. de la Rosa-Trevín, Joaquín Otón, Vahid Abrishami
Publikováno v:
Bioinformatics. 29:2460-2468
Motivation: Structural information of macromolecular complexes provides key insights into the way they carry out their biological functions. Achieving high-resolution structural details with electron microscopy requires the identification of a large