Zobrazeno 1 - 10
of 45
pro vyhledávání: '"J.M. Talent"'
Publikováno v:
Biochemistry. 47:5657-5667
During interaction of actin with myosin, cross-bridges impart mechanical impulses to thin filaments resulting in rotations of actin monomers. Impulses are delivered on the average every tc seconds. A cross-bridge spends a fraction of this time (ts) s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f6095d5df840082e48ecb25f3d0447eb
https://doi.org/10.1021/bi7023223
https://doi.org/10.1021/bi7023223
Publikováno v:
Experimental Biology and Medicine. 231:28-38
The ability to measure properties of a single cross-bridge in working muscle is important because it avoids averaging the signal from a large number of molecules and because it probes cross-bridges in their native crowded environment. Because the con
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff78f4b6120555a58a05c74ac6343c6c
https://doi.org/10.1177/153537020623100104
https://doi.org/10.1177/153537020623100104
Autor:
Julian Borejdo, Thomas P. Burghardt, J.M. Talent, A. Shepard, D. Dumka, Irina Akopova, Ari Malka
Publikováno v:
Biophysical Journal. 86(4):2308-2317
It is well documented that muscle contraction results from cyclic rotations of actin-bound myosin cross-bridges. The role of actin is hypothesized to be limited to accelerating phosphate release from myosin and to serving as a rigid substrate for cro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::df0907fea2a6799cd46af39994681212
Autor:
Susan T. Weintraub, Christina A. Malakowsky, Robert W. Gracy, Craig C. Conrad, J.M. Talent, Joungil Choi, Christopher A. Carroll
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1637:135-141
We have examined the effects of the beta-amyloid peptide (Aβ 25–35 ) on fibroblasts derived from subjects with Alzheimer's disease (AD) and from age-matched controls. The peptide was significantly more cytotoxic to the AD-derived fibroblasts. The
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a452ff949eae141997c3143e607c021d
https://doi.org/10.1016/s0925-4439(02)00227-2
https://doi.org/10.1016/s0925-4439(02)00227-2
Autor:
Craig C. Conrad, Christina A. Malakowsky, Rong Dai, Susan T. Weintraub, J.M. Talent, Joungil Choi, Robert W. Gracy, Christopher A. Carroll
Publikováno v:
PROTEOMICS. 3:73-77
Oxidative damage to neuronal proteins appears to be central to the toxicity associated with a number of neuropathologies, including Alzheimer's disease. We have examined this by using oxidative stress to induce apoptosis in a mouse hippocampal neuron
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::10440724bc2f5fab7403bdcdc9b18286
https://doi.org/10.1002/pmic.200390011
https://doi.org/10.1002/pmic.200390011
Autor:
Priya Muthu, Irina Akopova, Ignacy Gryczynski, Julian Borejdo, Nils Calander, J.M. Talent, Tanya Shtoyko, Zygmunt Gryczynski
Publikováno v:
Journal of biomedical optics. 13(3)
Recent advances in detector technology make it possible to achieve single molecule detection (SMD) in a cell. SMD avoids complications associated with averaging signals from large assemblies and with diluting and disorganizing proteins. However, it r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::51bf176c181b99ac3c6fddaffed7b939
https://pubmed.ncbi.nlm.nih.gov/18601566
https://pubmed.ncbi.nlm.nih.gov/18601566
Autor:
J.M. Talent, P. Muthu, Zygmunt Gryczynski, Irina Akopova, Ignacy Gryczynski, Julian Borejdo, Kirti Jain
Publikováno v:
Analytical biochemistry. 366(2)
Recently it has become possible to study interactions between proteins at the level of single molecules. This requires collecting data from an extremely small volume, small enough to contain one molecule—typically of the order of attoliters (10−1
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8f54aeeb742331ea55551dc13cd8d79d
https://pubmed.ncbi.nlm.nih.gov/17531183
https://pubmed.ncbi.nlm.nih.gov/17531183
Publikováno v:
Biochimica et biophysica acta. 1763(2)
In order to measure the cycling of a few ( approximately 6) myosin heads in contracting skeletal muscle, myofibrils were illuminated by Total Internal Reflection and observed through a confocal aperture. Myosin heads rotated at a rate approximately e
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0845b9abd246d14f671bf64f1a8aaf87
https://pubmed.ncbi.nlm.nih.gov/16510199
https://pubmed.ncbi.nlm.nih.gov/16510199
Publikováno v:
Journal of muscle research and cell motility. 25(7)
The rotation of myosin heads and actin were measured simultaneously with an indicator of the enzymatic activity of myosin. To minimize complications due to averaging of signals from many molecules, the signal was measured in a small population residi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a5640816863920c2f7d7f1c7e91b660e
https://pubmed.ncbi.nlm.nih.gov/15711885
https://pubmed.ncbi.nlm.nih.gov/15711885
Publikováno v:
Analytical Biochemistry. 62:281-285
Methods are described which permit the recovery and analysis of peptides at the nanomole level in quantitative yield from cellulose thin layers. Although commercial celluloses are contaminated with significant levels of proteins which are not removed
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04c851a5e31526bf6c3ce35e42e6bbd8
https://doi.org/10.1016/0003-2697(74)90388-1
https://doi.org/10.1016/0003-2697(74)90388-1