Zobrazeno 1 - 10
of 38
pro vyhledávání: '"J.J. Michniewicz"'
Autor:
Stéphane Bernatchez, Marie-France Karwaski, Eduardo N. Taboada, Anna-Maria Cunningham, Warren W. Wakarchuk, Michel Gilbert, N. Martin Young, J.J. Michniewicz
Publikováno v:
Journal of Biological Chemistry. 277:327-337
We have compared the lipo-oligosaccharide (LOS) biosynthesis loci from 11 Campylobacter jejuni strains expressing a total of 8 different ganglioside mimics in their LOS outer cores. Based on the organization of the genes, the 11 corresponding loci co
Autor:
Warren W. Wakarchuk, Michel Gilbert, N. Martin Young, J.J. Michniewicz, Jean-Robert Brisson, Marie-France Karwaski, Anna-Maria Cunningham, Yuyang Wu
Publikováno v:
Journal of Biological Chemistry. 275:3896-3906
We have applied two strategies for the cloning of four genes responsible for the biosynthesis of the GT1a ganglioside mimic in the lipooligosaccharide (LOS) of a bacterial pathogen,Campylobacter jejuni OH4384, which has been associated with Guillain-
Autor:
David R. Bundle, Su-Jun Deng, J.J. Michniewicz, J. Sadowska, N. M. Young, Saran A. Narang, C. R. Mackenzie
Publikováno v:
Journal of Biological Chemistry. 269:9533-9538
A single-chain variable fragment (Fv) version of a murine monoclonal antibody, Se155-4, specific for Salmonella serogroup B O-polysaccharide, was used as a model system for testing monovalent phage display as a route for enhancing the relatively low
Autor:
J.J. Michniewicz, Ginette Dubuc, Fei-Long Yao, Rajmund L. Somorjai, Saran A. Narang, J. Phipps
Publikováno v:
Biochemical and Biophysical Research Communications. 149:340-346
A DNA of 495 bp coding for T4-lysozyme was chemically synthesized and cloned in Escherichia coli. On DNA sequence analysis, clones pTLY.10 and pTLY.9 were identified to contain identical and complete T4-lysozyme coding sequences except that pTLY.9 ha
Publikováno v:
Biochemical and Biophysical Research Communications. 145:190-195
DNA of 235 b.p. coding for N-terminal domain (1–78) T4-lysozyme was synthesized and cloned by ligating twelve synthetic fragments with a linearized plasmid pUCE8 followed by transformation. On expression in E. coli strain JM103 cells, colonies cont
Publikováno v:
Nucleic Acids Research. 6:1371-1385
Preparative reversed-phase thin layer chromatography on silanized silica-gel (RP-2 and RP-18) has been developed to purify triester deoxyribooligonucleotides prepared by the modified triester method. The effectiveness of this technique has been demon
Autor:
J.J. Michniewicz, Ahmad I. Bukhari, Thomas A. Patterson, Donald L. Court, Saran A. Narang, Ginette Dubuc, J. Goodchild
Publikováno v:
Gene. 50:101-109
We describe below the chemical synthesis of the right and left ends of bacteriophage Mu and characterize the activity of these synthetic ends in mini-Mu transposition. Mini-Mu plasmids were constructed which carry the synthetic Mu ends together with
Autor:
J.J. Michniewicz, Saran A. Narang
Publikováno v:
Analytical Biochemistry. 49:379-392
Thin-layer chromatography on Avicel-cellulose plates has been developed for (i) efficient and easy fractionation of complex chemical reaction mixture such as polymerized thymidine 5′-phosphate, (ii) characterization of oligonucleotides, (iii) in si
Publikováno v:
Journal of the American Chemical Society. 91:936-943
Publikováno v:
Journal of Chromatography A. 85:159-161
The authors have exploited silica gel thin-layer chromatography using common aqueous solvent systems for rapid resolution and identification of oligonucleotides up to decanucleotides. Chemically polymerized thymidine 5'-phosphate was used as a model