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Publikováno v:
Journal of Applied Microbiology. 121:1457-1468
Aims Persistence of Mycobacterium bovis was investigated in UK raw milk cheeses. Methods and Results Replicating traditional cheese production methods under stringent CL3 containment conditions, Cheddar and Caerphilly cheeses were produced with Myco.
Publikováno v:
Journal of Applied Microbiology. 110:479-489
Aims: To develop a quantitative PCR assay for sensitive and specific detection of Mycobacterium avium ssp. paratuberculosis (Map) in a range of dairy products. Methods and Results: TaqMan® assays were designed to target the IS900 and f57 genetic ele
Autor:
Frans Pieter Cilliers, J.A. Donaghy, M.T. Rowe, Pieter A. Gouws, James C. Johnston, M. Keyser
Publikováno v:
Letters in Applied Microbiology. 49:217-221
Aims: To determine the effect of UV radiation on the viability of two strains of Mycobacterium avium ssp. paratuberculosis (Map) inoculated into milk. Methods and Results: Mycobacterium avium ssp. paratuberculosis in a ultra heat treated milk matrix
Autor:
V. De Jonghe, E. Vindel, J.A. Donaghy, B. Blanchard, Lieve Herman, K. Duhem, P. Hammer, M.T. Rowe, J.L.W. Rademaker
Publikováno v:
Food Microbiology. 25:128-135
There is a need for standardised, robust, reproducible molecular and culture methods to achieve clarification of the inactivation of Mycobacterium avium subsp. paratuberculosis (Map), the causative microbial agent of Johne's disease, in (faecally) co
Publikováno v:
Letters in applied microbiology. 59(4)
Reports have highlighted the absence of contemporary peer reviewed publications pertaining to Mycobacterium bovis culture from raw milk and cheese. By replicating traditional methods, cheese-making methodology and equipment were devised to produce Ch
Autor:
J.A. Donaghy, A.M. McKay
Publikováno v:
Journal of Applied Bacteriology. 76:506-510
Samples of orange peel were inoculated with the yeast Kluyveromyces fragilis and the yield and composition of the extracted pectin was monitored over a 3 d fermentation period. A maximum yield of 12.8% from dry albedo pulp was obtained after 24 h. Th
Autor:
A.M. McKay, J.A. Donaghy
Publikováno v:
Bioresource Technology. 47:25-28
Dried sweet whey was used as a complete medium for the production of polygalacturonase by the yeast Kluveromyces fragilis. The optimum concentration for enzyme production was 0.5% (w/v) after two days at 25°C. Supplementation of whey with sodium pol
Autor:
J.A. Donaghy, A.M. McKay
Publikováno v:
Journal of Applied Bacteriology. 74:662-666
A strain of the yeast-like fungus Aureobasidium pullulans was grown on whey to produce an extracellular protease. The protease was totally inhibited by the serine inhibitor, phenyl methyl sulphonyl fluoride (PMSF), and partially inhibited by the chel
Detection of Salmonella in animal protein by Rappaport-Vassiliadis broth using indirect impediometry
Autor:
R.H. Madden, J.A. Donaghy
Publikováno v:
International Journal of Food Microbiology. 17:281-288
Indirect impedance methodology for the detection of Salmonella was investigated using a rapid automated bacterial impedance technique (RABIT) system. Four commercially available Rappaport-Vassiliadis (RV) enrichment broths were evaluated for their se