Zobrazeno 1 - 10
of 41
pro vyhledávání: '"J. William Efcavitch"'
Publikováno v:
Journal of Commercial Biotechnology. 25
This article provides an overview of the emerging technology of enzymatic DNA synthesis, which holds the promise of making the business of writing DNA cost-effective, faster, sustainable, and more accurate compared to the traditional DNA synthesis me
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e29fc5fef11a695f77abace6f2957ebc
https://doi.org/10.5912/jcb956
https://doi.org/10.5912/jcb956
Autor:
Tom Ellis, Bogumil J. Karas, Cintia M. Coelho, Axel Trefzer, Hille Tekotte, Scott C. Lenaghan, Alec A. K. Nielsen, Giovanni Stracquadanio, C. Neal Stewart, Alexander C. Smith, Marc Güell, Leslie A. Mitchell, Nili Ostrov, Bill J. Peck, Jacob Beal, J. William Efcavitch, D. Benjamin Gordon, Jeffery A. Schloss, Henry H. Lee, George M. Church, Joel S. Bader
Publikováno v:
Science. 366:310-312
Engineering biology with recombinant DNA, broadly called synthetic biology, has progressed tremendously in the last decade, owing to continued industrialization of DNA synthesis, discovery and development of molecular tools and organisms, and increas
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8620a87238dcc61d9071f2ce33922e35
https://doi.org/10.1126/science.aay0339
https://doi.org/10.1126/science.aay0339
Autor:
Aaron M. Leconte, Maha P. Patel, Lauryn E. Sass, Peter McInerney, Mirna Jarosz, Li Kung, Jayson L. Bowers, Philip R. Buzby, J. William Efcavitch, Floyd E. Romesberg
Publikováno v:
Angewandte Chemie. 122:6057-6060
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a40a7481540299573884ef4dbd24966b
https://doi.org/10.1002/ange.201001607
https://doi.org/10.1002/ange.201001607
Autor:
Philip R. Buzby, Suhaib Siddiqi, Peter McInerney, Kathleen E. Steinmann, Edyta Krzymanska-Olejnik, Jayson Bowers, John F. Thompson, Doron Lipson, Subramanian Marappan, J. William Efcavitch, Judith Mitchell, Marie Causey, Mirna Jarosz, Atanu Roy, Adam R. Platt, Eric Beer, Geoffrey M Lowman, Li Kung
Publikováno v:
Nature methods
Nucleotide analogs modified with a free 3′ hydroxyl, maintaining the interactions at the polymerase active site, and a cleavable linker, attaching a fluorescent dye and an inhibitor, are efficient at reading homopolymer runs in a single-molecule se
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2508d6e90b8cd3cee37d4c2c9acf1476
https://pubmed.ncbi.nlm.nih.gov/19620973
https://pubmed.ncbi.nlm.nih.gov/19620973
Autor:
Philip R. Buzby, Dan Lapen, Mirna Jarosz, Edward C. Thayer, Ido Braslavsky, Jayson Bowers, Keith A. Moulton, J. William Efcavitch, Howard Weiss, Marie Causey, Jaime Gill, Zheng Xie, Hazen P. Babcock, Stephen R. Quake, Rebecca Ward, John Healy, Eric Beer, Jennifer Colonell, Kathleen E. Steinmann, Eldar Giladi, Anastasia Tyurina, James Joseph Dimeo, Timothy D. Harris
Publikováno v:
Science (New York, N.Y.). 320(5872)
The full promise of human genomics will be realized only when the genomes of thousands of individuals can be sequenced for comparative analysis. A reference sequence enables the use of short read length. We report an amplification-free method for det
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::299debb6d6aa6c8521dcd737b5d525ca
https://pubmed.ncbi.nlm.nih.gov/18388294
https://pubmed.ncbi.nlm.nih.gov/18388294
Autor:
Barnett B. Rosenblum, H. Michael Wenz, J. William Efcavitch, James M. Robertson, Carla Wike, Steve Menchen, Frank Oaks, David M. Demorest, Don Scheibler, Dennis A. Gilbert
Genotyping, as applied to linkage mapping, human identification, or mapping of genetic traits, mandates electrophoretic separation systems that enable a user to identify alleles with high precision to obtain a correct genotype. For 2-bp microsatellit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cd98f8c352d858fb0ade665c74e61968
https://europepmc.org/articles/PMC310688/
https://europepmc.org/articles/PMC310688/
Publikováno v:
Journal of Chromatography A. 326:293-299
The small-pore C18 column materials commonly used for reversed-phase high-performance liquid chromatographic purification of synthetic oligodeoxyribonucleotides do not yield DNA sufficiently pure for some applications. Separation and purification on
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ed365e58e1b43d630f7af2d4022b31f5
https://doi.org/10.1016/s0021-9673(01)87454-9
https://doi.org/10.1016/s0021-9673(01)87454-9
Autor:
Cheryl R. Heiner, J. William Efcavitch
Publikováno v:
Nucleosides and Nucleotides. 4:267-267
The synthesis in our laboratory of very long (80–106 bases) oligodeoxynucleotides using the phosphite method of Matteucci and Caruthers in an automated DNA synthesizer indicates the true efficiency of this multiple step process. The coupling effici
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c616af8c6445e4759c25794c034449c8
https://doi.org/10.1080/07328318508077883
https://doi.org/10.1080/07328318508077883
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Publikováno v:
TechLife News; 3/4/2023, Issue 592, p224-235, 12p, 6 Color Photographs