Zobrazeno 1 - 10
of 51
pro vyhledávání: '"J. W. Pollard"'
Autor:
R. Aird, J. Wills, K. F. Roby, C. Bénézech, R. H. Stimson, M. Wabitsch, J. W. Pollard, A. Finch, Z. Michailidou
Publikováno v:
Frontiers in Endocrinology, Vol 13 (2022)
ObjectiveObesity increases the risk of certain cancers, especially tumours that reside close to adipose tissue (breast and ovarian metastasis in the omentum). The obesogenic and tumour micro-environment share a common pathogenic feature, oxygen depri
Externí odkaz:
https://doaj.org/article/2cfb855309504d65bd85eb239aa28c2c
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 4
Many techniques have been developed to transfect mammalian cells with DNA (1-2). The most commonly used method is to expose cells to a coprecipitate of DNA and calcium phosphate (3). This technique works very well with both genomic and recombinant DN
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::5034a450e12e771972d22fde3fdf52ee
https://pubmed.ncbi.nlm.nih.gov/21424646
https://pubmed.ncbi.nlm.nih.gov/21424646
Autor:
A N, Bateson, J W, Pollard
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 4
The ideal genomic library should consist of a series of clones containing overlapping sequences that are representative of the entire genome. Such an ideal state can be approached by cutting the DNA randomly and cloning large pieces of this DNA into
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::f31ec67f7463f9607d2c0f9156b4eba6
https://pubmed.ncbi.nlm.nih.gov/21424637
https://pubmed.ncbi.nlm.nih.gov/21424637
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 4
Northern blotting is a way of detecting specific RNA sequences, homologous to a hybridization probe, within a complex RNA sample. In this procedure RNA is first separated by electrophoresis under denaturing conditions (see Chapter 1 ), followed by bl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::deb259eee615ee0b2440c4accf09180a
https://pubmed.ncbi.nlm.nih.gov/21424622
https://pubmed.ncbi.nlm.nih.gov/21424622
Autor:
J W, Pollard, M J, Clemens
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 4
The most commonly used systems to translate mRNAs in vitro are the rabbit reticulocyte lysate and the wheat germ extract (1,2 and see Vol. 2 in this series). Both these systems have a number of advantages, including the ease and cheapness of preparat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::545b0788580b696f4f48fb6c340bf224
https://pubmed.ncbi.nlm.nih.gov/21424625
https://pubmed.ncbi.nlm.nih.gov/21424625
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 4
The first successful method for electrophoretic analysis of the full size range of cellular RNA molecules was described by Loening (1), and its introduction allowed for major advances, most particularly in the molecular biology of eukaryotic organism
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::be26a2112d4205811233aa9808f7a81a
https://pubmed.ncbi.nlm.nih.gov/21424621
https://pubmed.ncbi.nlm.nih.gov/21424621
Autor:
R, Jagus, J W, Pollard
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 3
Immunoprobing for specific peptides on solid supports such as nitrocellulose requires prior blocking of nonspecific protein binding sites. Many proteins, or protein mixtures, have been used for this, including bovine serum albumin, gelatin, and calf
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::3a5f8bc1716985f76e2fb8f46e1294a6
https://pubmed.ncbi.nlm.nih.gov/21400179
https://pubmed.ncbi.nlm.nih.gov/21400179
Autor:
J W, Pollard
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 5
This article will describe the basic techniques required for successful cell culture. It will also act to introduce some of the other chapters in this volume. It is not intended, as this volume is not, to describe the establishment of a tissue cultur
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::0ad30911c7388ae74026634656c6a823
https://pubmed.ncbi.nlm.nih.gov/21374110
https://pubmed.ncbi.nlm.nih.gov/21374110
Autor:
J W, Pollard
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1
Autoradiography (see Chapter 17 and Vol. 2) offers a convenient, quick, wand cheap means of quantifying proteins separated by gel electrophoresis.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::283280ce067f1e0a91bb4c37b8d78a57
https://pubmed.ncbi.nlm.nih.gov/20512676
https://pubmed.ncbi.nlm.nih.gov/20512676
Autor:
J W, Pollard
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1
Since O'Farrell (1) introduced the improved technique for high resolution two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), it has become one of the most powerful tools for the separation and quantification of proteins from complex mixtu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::8e203e1b5ac67c46badf583aa603ccf1
https://pubmed.ncbi.nlm.nih.gov/20512677
https://pubmed.ncbi.nlm.nih.gov/20512677