Zobrazeno 1 - 10
of 51
pro vyhledávání: '"J. Upshaw-Earley"'
Publikováno v:
Biochemical and Biophysical Research Communications. 304:22-25
The presence of caveolin-3 was reinvestigated in smooth muscle cells in situ and in culture. Immunocytochemistry of aortic strips showed that both caveolins-1 and -3 are present, but that caveolin-3 is considerably less abundant and detected in only
Autor:
J Upshaw-Earley, Ernest Page, Donald D. Doyle, H. Clive Palfrey, G. E. Goings, Barbara Ranscht
Publikováno v:
Journal of Biological Chemistry. 273:6937-6943
Sucrose-density flotation analysis of Triton-insoluble membrane domains isolated from highly purified sheep ventricular sarcolemma revealed the presence of two major 120- and 100-kDa proteins. Both species migrated in two-dimensional isoelectric focu
Publikováno v:
Circulation Research. 81:86-91
Abstract We have previously shown that atrial natriuretic peptide (ANP) is present in caveolae of in situ rat atrial myocytes. To investigate whether intracaveolar ANP of rat atrial myocytes exists within caveolae bound to type B ANP receptors (ANP-R
Publikováno v:
Circulation Research. 75:949-954
The plasma membrane-associated non-clathrin-coated vesicles called caveolae are multifunctional organelles thought to be implicated in the sequestration and transport of small molecules (potocytosis) as well as in the binding of Ca2+ ions, signal tra
Publikováno v:
American journal of physiology. Heart and circulatory physiology. 282(6)
We assessed the cellular localization and relative concentration of the C-type natriuretic peptide (CNP) guanylate cyclase-B (GC-B) receptor in the adult rat heart ventricle by several techniques. In frozen sections of the ventricle, anti-receptor an
Autor:
Jeffrey Winterfield, J Upshaw-Earley, Ernest Page, G. E. Goings, Donald D. Doyle, Amir L. Bastawrous
Publikováno v:
The American journal of physiology. 274(6)
We show by confocal immunofluorescence microscopy that the water channel protein aquaporin-1, not previously identified within cardiomyocytes, localizes at 20 and 37°C to rat cardiomyocyte sarcolemmal caveolar membrane and subsarcolemmal cytoplasm o
Publikováno v:
Circulation research. 75(2)
The time course of endocytic uptake of Lucifer yellow (LY) was followed by fluorescence and electron microscopy after exposure of primary cultures of atrial myocytes from adult rats to LY under conditions that prevented transplasmalemmal LY entry via
Publikováno v:
Circulation research. 71(1)
Atrial distension, which stimulates atrial natriuretic peptide secretion by atrial myocytes, also stretches nonmuscle cells. In a noncontracting in vitro preparation of combined right and left atria we demonstrated by electron microscopy that, at 37
Inhibition of atrial peptide secretion at different stages of the secretory process: Ca2+ dependence
Publikováno v:
The American journal of physiology. 261(6 Pt 1)
We have used a noncontracting in vitro preparation of stretched and unstretched rat atria to estimate contributions of constitutive and regulated pathways to the rates of stretch-augmented and basal secretion of immunoreactive atrial natriuretic pept
Publikováno v:
The American journal of physiology. 259(5 Pt 1)
Absolute rates of immunoreactive atrial natriuretic peptide (ANP) secretion were measured in vitro at 37 degrees C in noncontracting preparations of combined right and left rat atria at constant distending pressures of 0 or 5.1 mmHg in presence of 0.