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THE covalent binding of a haptene to its specific antibody with a view to identifying the section of peptide chains which form the combining site has been pioneered by Singer et al.1,2, who named this approach affinity labelling. Antibodies were prep
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6e40f70c774e40d9a2a4af0026733260
https://doi.org/10.1038/224511a0
https://doi.org/10.1038/224511a0
Autor:
R. D. Sammons, James A. Sikorski, T. Widlanski, Joel E. Ream, J. R. Knowles, Karen S. Anderson, Paul D. Pansegrau
Publikováno v:
ChemInform. 23
Autor:
R, Knowles, J R, Knowles
Publikováno v:
Health estate. 52(3)
Publikováno v:
Aviation, space, and environmental medicine. 68(12)
Under the auspices of the European Command (EUCOM) Military-to-Military Exchange Program, the authors participated in 13 trips to visit aeromedical facilities of nine Eastern European nations (Albania, Belarus, Bulgaria, Czech Republic, Hungary, Lith
Publikováno v:
The Journal of biological chemistry. 266(35)
Infection by influenza virus is initiated by a cellular adhesion event that is mediated by the viral protein, hemagglutinin, which is exposed on the surface of the virion. Hemagglutinin recognizes and binds to cell surface sialic acid residues. Altho
Publikováno v:
Radiology. 155:159-162
The user-selectable magnetic resonance imaging parameters involved in a multi-section multi-echo pulse sequence are described and their interrelationships explained. These parameters include repetition time (TR), echo delay time (TE), the number of s
The stereochemical course at phosphorus of the reaction catalyzed by phosphoenolpyruvate carboxylase
Autor:
J R Knowles, D E Hansen
Publikováno v:
Journal of Biological Chemistry. 257:14795-14798
[(S)-16O,17O]Thiophosphoenolpyruvate has been used as a substrate in H218O for the reaction catalyzed by phosphoenolpyruvate carboxylase, and the absolute configuration of the product, inorganic [16O,17O,18O]thiophosphate, has been determined. The re
Publikováno v:
Journal of Biological Chemistry. 263:16069-16072
Adenosine [gamma-(S)-16O,17O,18O]triphosphate was used as substrate in the phosphokinase reaction catalyzed by 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase. The product D-fructose 2,6-[2-16O,17O,18O]bisphosphate was then used as substrate in
Autor:
J. R. Knowles, Martin R. Webb
Publikováno v:
Biochemical Journal. 141:589-592
In the presence of triose phosphate isomerase, the substrate dihydroxyacetone phosphate is reduced stereoselectively by NaBH4. The reduction of enzyme-bound substrate is almost completely or completely stereoselective and occurs about one order of ma
Autor:
J. R. Knowles
Publikováno v:
Pure and Applied Chemistry. 56:1005-1010
Stereoselectively labeled samples of E—2an Z—[9—2H,3H]— chorismate have been synthesized from Fand z-[3H, Hlphosphoen6lpyruvate by the enzymes of the shikimate pathway. The position of the tritium label at the 9—position of chorismate has b