Zobrazeno 1 - 10
of 244
pro vyhledávání: '"J. R. Bertino"'
Autor:
R. Arriagada, J. R. Bertino, N. M. Bleehen, O. Brodin, R. Feld, J. H. Goldie, H. H. Hansen, D. C. Ihde, T. Le Chevalier, R. L. Souhami, null and the Workshop Participants
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5c272c5638bb035a33707c3a7ae525c3
https://doi.org/10.1159/000416210
https://doi.org/10.1159/000416210
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 15
One of the greatest potentials of polymerase chain reaction (PCR) lies in the fact that even minute amounts of target DNA or extensively damaged DNA can be successfully amplified in vitro and thus become amenable to further study. This enables a deta
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 15
Identification of point mutations has been facilitated by a number of techniques, including transfection assays, oligonucleotide hybridization, electrophoretic migration of heteroduplexes, RNase mismatch analysis, direct sequencing, and DNA-polymeras
Autor:
J R Bertino, Diane E. McCloskey, B G Rowan, Cynthia A. Russell, Enrico Mini, John J. McGuire, G. Pizzorno
Publikováno v:
Journal of Biological Chemistry. 266:6181-6187
Determinants of methotrexate (MTX) resistance in cell lines resistant to short, but not continuous, MTX exposure were investigated since such lines may have relevance to clinical resistance. CCRF-CEM R30dm (R30dm), cloned from CCRF-CEM R30/6 (a MTX-r
Publikováno v:
Clinical cancer research : an official journal of the American Association for Cancer Research. 7(10)
Ecteinascidin 743 (ET-743) is a potent antitumor agent from the Caribbean tunicate Ecteinascidin turbinata and is presently in clinical trials for human cancers. To better understand how ET-743 might be used clinically, the present study used SRB ass
Autor:
W W, Li, N, Takahashi, S, Jhanwar, C, Cordon-Cardo, Y, Elisseyeff, J, Jimeno, G, Faircloth, J R, Bertino
Publikováno v:
Clinical cancer research : an official journal of the American Association for Cancer Research. 7(9)
The cytotoxic effects of ecteinascidin-743(ET-743), a novel marine natural product, were evaluated and compared with that of clinically used anticancer agents methotrexate, doxorubicin, etoposide, and paclitaxel in eight human soft tissue sarcoma (ST
Publikováno v:
Molecular medicine (Cambridge, Mass.). 7(3)
BACKGROUND: The G52S mutation in the Arg50 loop of thymidylate synthase leads to decreased binding of FdUMP. It has been suggested that the mutation affects the Arg50 residue (within the Arg50 loop) responsible for binding the phosphate of FdUMP. The
Publikováno v:
Cancer research. 61(11)
Intracellular metabolism of methotrexate (MTX) to MTX-polyglutamates (MTXPG) is one determinant of cytotoxicity. Steady-state accumulation of MTXPG seems to depend on the activity of two enzymes: folylpolyglutamate synthetase (FPGS), which adds gluta
Publikováno v:
Cancer research. 61(6)
We examined the effects of flavopiridol (FP), a cyclin-dependent kinase inhibitor, on doxorubicin (DOX)-induced cell killing in an osteosarcoma cell line (SaOs-2) that lacks functional retinoblastoma protein (pRb). The IC50 value for DOX was 7-fold l
Publikováno v:
Cancer research. 61(5)
IgG polyclonal antiserum was generated in New Zealand White rabbits immunized with a 16-mer peptide consisting of a specific amino acid sequence at residues corresponding to the sixth to seventh predicted transmembrane domain of the human reduced fol