Zobrazeno 1 - 10
of 34
pro vyhledávání: '"J. O. Carlson"'
Autor:
Ken E. Olson, Irma Sanchez-Vargas, Zach N. Adelman, Barry J. Beaty, Carol D. Blair, J. O. Carlson, Emily A. Travanty
Publikováno v:
Journal of Virology. 76:12925-12933
Double-stranded RNA (dsRNA) initiates cellular posttranscriptional responses that are collectively called RNA silencing in a number of different organisms, including plants, nematodes, and fruit flies. In plants, RNA silencing has been associated wit
Publikováno v:
Aquatic Toxicology. 54:249-260
Metal pollution of aquatic ecosystems is a problem of economic and health importance. Sensitive molecular biomarkers of metal exposure are sorely needed. We have isolated a cDNA from the midge Chironomus tentans that is transcribed in all organs and
Publikováno v:
Insect Molecular Biology. 10:265-273
Aedes aegypti were injected intrathoracically with double subgenomic Sindbis (dsSIN) viruses with inserted sequences derived from the genome of one or more of the four dengue (DEN) virus serotypes. Mosquitoes were highly resistant to challenge with h
Publikováno v:
Archives of Virology. 143:1365-1377
The Sindbis replicon expression system was used to express La Crosse (LAC) virus envelope glycoprotein genes in both mammalian and mosquito cell culture. Replicon expressed LAC proteins had correct molecular mass (Mr) and were antigenically similar t
Publikováno v:
Insect Biochemistry and Molecular Biology. 27:423-429
Mosquito salivary glands play an important role in the transmission of arthropod-borne pathogens. The ability to express genes in mosquitoes would be a powerful approach to characterize salivary gland genes, and to reveal important vector determinant
Autor:
J. O. Carlson, Laura Klimowski, Barry J. Beaty, Ken E. Olson, John D. Tessari, Ray S. H. Yang, A. Rayms-Keller
Publikováno v:
Environmental Toxicology and Chemistry. 15:85-91
We have developed a molecular bioreporter model for detecting an invertebrate response to heavy metals. The bioreporter system, pMt2-luc, utilizes a Drosophila melanogaster metallothionein promoter to regulate luciferase expression in stably transfor
Autor:
Ken E. Olson, Carol D. Blair, S. Higgs, K. I. Kamrud, A.M. Powers, Barry J. Beaty, J. O. Carlson
Publikováno v:
Experimental Parasitology. 81:394-403
Sindbis (SIN) replicon virus was used to express chloramphenicol acetyltransferase (CAT) in Aedes albopictus (C6/36) cells and Aedes triseriatus mosquitoes. RNA transcribed in vitro from a SIN replicon plasmid (pSINrep5/CAT) and from SIN virus helper
Autor:
A.M. Powers, Barry J. Beaty, K. I. Kamrud, S. Higgs, J. O. Carlson, A. Rayms-Keller, Ken E. Olson
Publikováno v:
Insect Molecular Biology. 4:245-251
A recombinant Sindbis virus, TE/3'2J/ANTI-S, containing LaCrosse virus small segment cDNA in antisense orientation, was inoculated into Aedes triseriatus mosquitoes. Virus replication and LAC-ANTI-S RNA expression were analysed temporally and spatial
Autor:
R. D. Possee, Barry J. Beaty, S. Higgs, A.M. Powers, J. O. Carlson, L. Klimowski, Ken E. Olson
Publikováno v:
Insect Molecular Biology. 4:97-103
The scorpion, Androctonus australis Hector, produces an insect-specific toxin (AaHIT) encoded by the Scotox gene. To assess the toxicity of AaHIT for mosquitoes, we have taken a novel approach to express the Scotox gene in vivo. We have engineered a
Publikováno v:
Annual Review of Entomology. 40:359-388
Despite their central role in disease transmission, relatively little is known of the molecular biology of arthropod vectors. Modern molecular approaches will undoubtedly provide considerable information about gene regulation and expression in vector