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Large Scale Refolding and Purification of the Catalytic Domain of Human BACE-2 Produced in E. coli

Autor: A. M. Mildner, R. L. Heinrikson, H. D. Fischer, J. M. Lull, Thomas L. Emmons, Alfredo G. Tomasselli, J. W. Leone

Publikováno v: Protein & Peptide Letters. 16:121-131

Evidence for a key role of beta-amyloid (Abeta) in Alzheimer's disease has led to considerable interest in potential therapeutic strategies targeting enzymes involved in processing the amyloid precursor protein (APP). Beta-site APP Cleaving Enzyme (B

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::153494af09ae75bfc3776dbf2125b283
https://doi.org/10.2174/092986609787316180

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High Yield Expression of Human BACE Constructs in Eschericia coli for Refolding, Purification, and High Resolution Diffracting Crystal Forms

Publikováno v: Protein & Peptide Letters. 15:131-143

BACE (beta-site APP cleaving enzyme) or beta-secretase, the enzyme responsible for processing APP to give the N-terminal portion of the Abeta peptide, is a membrane bound aspartyl protease consisting of an ectodomain catalytic unit, a C-terminal tran

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2ef55a7c8d6ffd247809d360890a6e53
https://doi.org/10.2174/092986608783489553

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The HIV-1 Protease as Enzyme and Substrate: Mutagenesis of Autolysis Sites and Generation of a Stable Mutant with Retained Kinetic Properties

Autor: D J Rothrock, Carol A. Bannow, C S Tomich, J M Lull, A. M. Mildner, I M Reardon, J L Sarcich, Clark W. Smith, W J Howe, Joseph W. Leone

Publikováno v: Biochemistry. 33:9405-9413

Site-directed mutagenesis of autolysis sites in the human immunodeficiency virus type 1 (HIV-1) protease was applied in an analysis of enzyme specificity; the protease served, therefore, as both enzyme and substrate in this study. Inspection of natur

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d1c53661bef00e283b8c61084e47454c
https://doi.org/10.1021/bi00198a005

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