Zobrazeno 1 - 6
of 6
pro vyhledávání: '"J. M. Guisan"'
Autor:
João Atílio Jorge, Gloria Fernández-Lorente, Luiz Estevam Cavenage Filó, J. M. Guisan, Ana Claudia Vici, Marita Gimenez Pereira, Fernanda Dell Antonio Facchini, Benevides C. C. Pessela, Maria de Lourdes Teixeira de Moraes Polizeli, Aline M. Polizeli
Publikováno v:
Digital.CSIC. Repositorio Institucional del CSIC
instname
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
instname
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Hypocrea pseudokoningii purified lipase was immobilized on hydrophobic supports (phenyl-sepharose, butyl-sepharose, octyl-sepharose, Hexyl Toyopearl, Lewatit, Purolite, Decaoctyl sepabeads) and ionic supports (Duolite, DEAE-agarose, PEI-agarose, MANA
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6578415d642e0cd246c6cb60c9724bd7
https://doi.org/10.1016/j.procbio.2014.12.027
https://doi.org/10.1016/j.procbio.2014.12.027
Autor:
S, Moreno-Pérez, A H, Orrego, M, Romero-Fernández, L, Trobo-Maseda, S, Martins-DeOliveira, R, Munilla, G, Fernández-Lorente, J M, Guisan
Publikováno v:
Methods in enzymology. 571
This chapter describes the physicochemical coating of the surface of immobilized enzymes with a dense layer of polyethylene glycol (PEG) to improve enzyme stability. One hypothesis is that a dense, viscous, polar PEG layer around the enzyme would enh
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::34d1e2637ddc9d23cc9562e5a763a5f7
https://pubmed.ncbi.nlm.nih.gov/27112394
https://pubmed.ncbi.nlm.nih.gov/27112394
Publikováno v:
Biotechnology and bioengineering. 76(3)
Epoxy supports covalently immobilize proteins following a two-step mechanism; that is, the protein is physically adsorbed and then the covalent reaction takes place. This mechanism has been exploited to combine the selectivity of metal chelate affini
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::b8a0f701663f9a1e71b6421c56cad5d6
https://pubmed.ncbi.nlm.nih.gov/11668463
https://pubmed.ncbi.nlm.nih.gov/11668463
Autor:
C, Mateo, G, Fernandez-Lorente, B C, Pessela, A, Vian, A V, Carrascosa, J L, Garcia, R, Fernandez-Lafuente, J M, Guisan
Publikováno v:
Journal of chromatography. A. 915(1-2)
New immobilized metal ion affinity chromatography (IMAC) matrices containing a high concentration of metal-chelate moieties and completely coated with inert flexible and hydrophilic dextrans are here proposed to improve the purification of polyhistid
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::83e50a686ec8404f99ac3466f75718aa
https://pubmed.ncbi.nlm.nih.gov/11358266
https://pubmed.ncbi.nlm.nih.gov/11358266
Publikováno v:
Biotechnology and applied biochemistry. 24(2)
Parameters relevant to the thermodynamically controlled synthesis of cephalothin utilizing highly active stabilized penicillin G acylase derivatives were studied. These included solubility/stability of substrates, enzyme derivative activity/stability
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::7c7ef048ae31b2d4ba6852e57acc32c3
https://pubmed.ncbi.nlm.nih.gov/8865605
https://pubmed.ncbi.nlm.nih.gov/8865605
Autor:
P J Scully, L Betancor, J Bolyo, S Dzyadevych, J M Guisan, R Fern, N Jaffrezic, G Kuncov, V Mat, B O, O Podrazky, K Rose, L Sasek, J S Young
Publikováno v:
Measurement Science & Technology; Oct2007, Vol. 18 Issue 10, p3177-3186, 10p