Zobrazeno 1 - 10
of 12
pro vyhledávání: '"J. M. A. Verbakel"'
Publikováno v:
Yeast. 9:787-795
In order to determine whether the changes in the activities and mRNA levels of enzymes involved in intermediary carbon metabolism previously observed in glucose-limited continuous cultures (Sierkstra et al., 1992a) were glucose specific, we have anal
Autor:
J. M. M. Verbakel, Anneke Westerhof, L. H. Elvers, Daan W. Notermans, S.C. de Greeff, Marcel F. Peeters, Frits R. Mooi, Joop F. P. Schellekens, C. J. Heuvelman, H.E. de Melker
Publikováno v:
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America. 50(10)
Background. We conducted a population-based, nation-wide, prospective study to identify who introduced pertussis into the household of infants aged ≤6 months admitted to the hospital for pertussis in the Netherlands. Methods. During the period 2006
Publikováno v:
Journal of General Microbiology. 138:2559-2566
SUMMARY: mRNA steady-state levels and activities of enzymes of intermediary carbon metabolism (hexokinase, phosphoglucoisomerase, phosphofructokinase, glucose-6-phosphate dehydrogenase, phosphoglucomutase) and glucose-regulated enzymes (pyruvate deca
Autor:
Rosemary A. Veale, Peter E. Sudbery, C. Theo Verrips, Nico Overbeeke, Isaac J. Bom, Arthur J. Fellinger, J. M. A. Verbakel
Publikováno v:
Yeast. 7:463-473
The methylotrophic yeast Hansenula polymorpha, a host organism for the production of heterologous proteins, has been applied to produce the alpha-galactosidase from the plant Cyamopsis tetragonoloba (guar). The yeast/Escherichia coli shuttle expressi
Publikováno v:
Current Genetics. 19:81-87
For the methylotrophic yeast, Hansenula polymorpha, expression vectors with different origins of replication have been constructed in order to analyse their influence on transformation and integration efficiency. The constructed plasmids are identica
Autor:
J. M. A. Verbakel, C. Theo Verrips, Walter Maurits M Verhue, RenéA. Driedonks, Arthur J. Fellinger
Publikováno v:
Journal of Structural Biology. 104:128-133
The interrelationships between various scientific and technological disciplines in food biotechnology are discussed briefly. In particular, emphasis is put on the roles that basic sciences play in biotechnological developments. This is illustrated by
Publikováno v:
Yeast (Chichester, England). 11(9)
The antifreeze peptide AFP6 from the polar fish Pseudopleuronectus americanus has been expressed in and secreted by the yeast Saccharomyces cerevisiae as a biologically active molecule. The gene for the 37 amino acid long peptide has been chemically
Autor:
Holger Y. Toschka, Linda Kox, Rudi J. Planta, Ruud H. Geerse, Arno T. A. Mooren, J. M. A. Verbakel, Theo C. Bootsman, Ronald J. M. Bergkamp
Publikováno v:
Applied microbiology and biotechnology. 40(2-3)
For expression of the alpha-galactosidase gene from Cyamopsis tetragonoloba in Kluyveromyces marxianus CBS 6556 we have used the promoter of the homologous inulinase-encoding gene (INU1). The INU1 gene has been cloned and sequenced and the coding reg
Publikováno v:
Yeast (Chichester, England). 9(6)
The URA3 gene, coding for orotidine-5′-phosphate decarboxylase, from Kluyveromyces marxianus CBS 6556, was isolated from a genomic DNA library. The K. marxianus URA3 gene encodes a protein of 267 amino acids with a calculated molecular weight of 29
Publikováno v:
European journal of biochemistry. 214(1)
Wild-type Saccharomyces cerevisiae and a strain carrying a deletion in the glucose-6-phosphate-isomerase gene (pgi1) were grown in carbon-limited continuous cultures on a mixture of fructose and galactose. Pulses of glucose, fructose and galactose we