Zobrazeno 1 - 10
of 15
pro vyhledávání: '"J. L. Spudich"'
Publikováno v:
Neuroscience and Behavioral Physiology. 49:163-168
Channelrhodopsins are retinylidene proteins unique in their ability to generate light-gated passive ion currents across the membrane. Heterologous expression of channelrhodopsins in animal cells, most notably, neurons, enables precise millisecond-sca
Publikováno v:
Журнал высшей нервной деятельности им И П Павлова. :9-17
Autor:
S, Dassarma, S P, Kennedy, B, Berquist, W, Victor Ng, N S, Baliga, J L, Spudich, M P, Krebs, J A, Eisen, C H, Johnson, L, Hood
Publikováno v:
Photosynthesis research. 70(1)
Halobacterium species display a variety of responses to light, including phototrophic growth, phototactic behavior, and photoprotective mechanisms. The complete genome sequence of Halobacterium species NRC-1 (Proc Natl Acad Sci USA 97: 12176-12181, 2
Publikováno v:
Journal of molecular biology. 308(2)
Sensory rhodopsins, phototaxis receptors in Haloarchaea, were purified and reconstituted into halobacterial lipids to form photoactive two-dimensional crystals. Images of vitreous ice-embedded, flattened, tubular crystals of sensory rhodopsin II (SRI
Autor:
J L, Spudich
Publikováno v:
Science (New York, N.Y.). 288(5470)
Publikováno v:
Biochemistry. 32(38)
Steric constraints in the retinal binding pocket of sensory rhodopsin I (SR-I) are analyzed by studying effects of sample temperature and retinal analogs. The flash-induced yield of the earliest detected intermediate S610, which corresponds to the K
Autor:
E N, Spudich, J L, Spudich
Publikováno v:
The Journal of biological chemistry. 268(22)
The sopI gene, which encodes the phototaxis receptor sensory rhodopsin I (SR-I), was expressed in Halobacterium salinarium strains with chromosomal deletions of (i) sopI only or of (ii) the region containing sopI and htrI. The htrI gene encodes a tra
Publikováno v:
Journal of Biological Chemistry. 258:7964-7967
Phase-lifetime spectrophotometry has been used to study the rate processes associated with intermediates in the photocycling pigments in membrane vesicles of mutant strains of Halobacterium halobium. Vesicles deficient in bacteriorhodopsin, but conta
Autor:
E N Spudich, J L Spudich
Publikováno v:
The Journal of Cell Biology
A photoregulated reversible protein phosphorylation system controlled by the halobacterial rhodopsins was recently reported. The results presented in this paper identify the initial steps in the pathway from the absorption of light to the photoregula
Autor:
E N, Spudich, J L, Spudich
Publikováno v:
The Journal of biological chemistry. 260(2)
Procedures are described for selectively radiolabeling the protein moiety (haloopsin) or the chromophoric prosthestic group (retinal) of the light-driven chloride pump halorhodopsin in intact cells of Halobacterium halobium. By sodium dodecyl sulfate