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Publikováno v:
Biologia plantarum. 48:185-191
For expression of MRJP1 - the most abundant protein of honeybee royal jelly - in plants, plasmid carrying the expression cassette composed of CaMV 35S RNA promoter, cDNA encoding MRJP1 with its native signal peptide, and nos3′ as transcription term
Publikováno v:
Journal of Apicultural Research. 35:63-68
SUMMARYThe previously published nucleotide sequence of complementary DNA (cDNA), coding for royal jelly protein RJP57-1, contained errors that were corrected by resequencing of the cDNA. The main error, the omission of a 108 bp (base pairs) long sequ
Publikováno v:
Journal of Molecular Biology. 244:665-672
Two new human cDNAs, designated phclk2 and phclk3, which have a high identity to the cDNA of the human protein kinase clk, were characterized. Typical features of hclk2 and hclk3 proteins are non-homologous N-terminal regions and the presence of the
Publikováno v:
DNA and Cell Biology. 13:1137-1143
From a cDNA sequence, we have deduced the amino acid sequence for a human amyloid precursor-like protein (APPH) with > 92% identity to the CDEI binding protein (CDEBP) of the mouse and the fragmentary rat protein YWK-II of unknown function. Expressio
Publikováno v:
Biochemical and Biophysical Research Communications. 199:962-968
RT PCR employing poly(A+)RNA from porcine luteal cells and a combination of primers designed from the known bovine MCP-1 cDNA identified the luteal cells as a source of MCP-1. This finding is corroborated by results from Northern analysis using total
Publikováno v:
Biochemical and Biophysical Research Communications. 197:1223-1228
From a λUNI-ZAP XR cDNA library derived from poly(A) + RNA of human ovarian granulosa cells a cDNA clone pHG51 was isolated. Sequence analysis showed significant homology to the C-terminal region of rat ribosomal protein L8 cDNA. The 5′-end of the
Publikováno v:
Biochemical and Biophysical Research Communications. 177:312-317
From a cDNA library in lambda gt11 derived from poly (A+)RNA of human ovarian granulosa cells a cDNA clone lambda HGP34, containing an EcoRI insert of 829 bp, was identified. After subcloning of the insert into pUC18, the clone pHGP34 was obtained an
Publikováno v:
FEBS letters. 528(1-3)
A peptide named apisimin was found in honeybee (Apis mellifera L.) royal jelly (RJ). N-terminal sequencing showed that this peptide corresponded to the sequence of a cDNA clone isolated from an expression cDNA library prepared from heads of nurse hon