Zobrazeno 1 - 10
of 20
pro vyhledávání: '"J. James Sedmak"'
Publikováno v:
Biotechnology Techniques. 4:107-112
The rapid, quantitative release of astaxanthin and other carotenoids from the yeast Phaffiarhodozyma is described. Hashed cells are ruptured with dimethylsulfoxide (DMSO) and carotenoids extracted into an organic solvent. Extraction and spectrophotom
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::98967ac51fea29b95b1e6e1e219a9113
https://doi.org/10.1007/bf00163282
https://doi.org/10.1007/bf00163282
Publikováno v:
Journal of Virological Methods. 6:99-105
Several Togaviridae of the alphavirus and flavivirus genera agglutinate trypsinized human group 0 erythrocytes (THOE) (Shortridge and Hu, 1976). Haemagglutinin titers of Semliki Forest virus (SFV) and Japanese encephalitis virus (JEV) measured with T
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::621f57d887f74cc86304bd8c9975406c
https://doi.org/10.1016/0166-0934(83)90073-3
https://doi.org/10.1016/0166-0934(83)90073-3
Autor:
J. James Sedmak, Sidney E. Grossberg
Publikováno v:
Analytical Biochemistry. 79:544-552
An assay for proteins in solution is described that depends on the conversion of Coomassie brilliant blue G250 in dilute acid from a brownish-orange to an intense blue color. As soon as the dye solution is mixed with a protein sample, the absorbance
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f7f0cf04853256985670cfb520fb37ae
https://doi.org/10.1016/0003-2697(77)90428-6
https://doi.org/10.1016/0003-2697(77)90428-6
Autor:
Sidney E. Grossberg, Vladimir M. Kushnaryov, J. James Sedmak, Robert F. Hevner, Hector S. MacDonald
Publikováno v:
Journal of Interferon Research. 6:247-250
Treatments of isolated L929 cell nuclei with MuIFN-β reduced the energy-dependent ef flux of 3H-RNA from these nuclei in a dose-dependent manner. These observations provide evidence for a direct physiological effect of IFN on the cell nucleus.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::25f3e5d81322163263035217db6fc3cc
https://doi.org/10.1089/jir.1986.6.247
https://doi.org/10.1089/jir.1986.6.247
Publikováno v:
Biochemical and Biophysical Research Communications. 157:109-114
Purified natural and recombinant murine gamma interferons (MuIFN-gamma) bind at 4 degrees C to cultured L929 mouse fibroblasts with comparable receptor-binding affinity (Kd = 9 x 10(-10) M). Both 125I-labeled MuIFNs are rapidly internalized by cells
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d46c502fc1e6f8ffc0d873721aaefca1
https://doi.org/10.1016/s0006-291x(88)80019-6
https://doi.org/10.1016/s0006-291x(88)80019-6
Autor:
Vladimir M. Kushnaryov, J. James Sedmak, Janet Debruin, Hector S. MacDonald, Gregory P. Lemense, Sidney E. Grossberg
Publikováno v:
Journal of Interferon Research. 6:241-245
An electron microscopic technique employing postembedding immunolabeling was used to identify unmodified, intracellular MuIFN-beta molecules. The MuIFN-beta was rapidly internalized by receptor-dependent endocytosis and within three minutes a majorit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e9f062b56ff5a19a94bbaf54ba39b555
https://doi.org/10.1089/jir.1986.6.241
https://doi.org/10.1089/jir.1986.6.241
Publikováno v:
Experimental Biology and Medicine. 149:433-438
The production of neuraminidase by the recombinant influenza virus X7(F1) in human monkey, rabbit, hamster, mouse, and chicken cell cultures is inhibited by interferon. Described is a new enzyme assay for neuraminidase that can be applied to the bioa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0675adfc542675f112d42e32fb62417f
https://doi.org/10.3181/00379727-149-38822
https://doi.org/10.3181/00379727-149-38822
Publikováno v:
Science. 194:527-529
Extracts of cells treated with purified interferon discharge the amino acid previously esterified to viral RNA but fail to affect aminoacylated transfer RNA. A similar activity is also present in c...
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d7738bc42effd7a338fb756cdc28d2d
https://doi.org/10.1126/science.973135
https://doi.org/10.1126/science.973135
Autor:
Lois B. Epstein, Jon Weil, J. James Sedmak, Sidney E. Grossberg, Jan L. Sabran, Charles J. Epstein
Publikováno v:
Nature. 301:437-439
Human immune interferon (IFN-gamma) differs from leukocyte interferon (IFN-alpha) and fibroblast interferon (IFN-beta) in cell origin, inducing agents, physical and biological properties and amino acid sequence. These differences have led to interest
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b4545f3f51dcbf97e1fc23bebca005b
https://doi.org/10.1038/301437a0
https://doi.org/10.1038/301437a0
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 82(10)
Radioiodinated mouse interferon-beta (125I-MuIFN-beta) bound with high affinity (Kd = 9.8 X 10(-10) M) to plasma membrane of L929 murine fibroblasts (4-6 X 10(3) receptor sites per cell). The binding was saturable and inhibited by a 100-fold excess o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5f732b5001d3be7d2e5e5b8bb439592c
https://pubmed.ncbi.nlm.nih.gov/3159015
https://pubmed.ncbi.nlm.nih.gov/3159015