Výsledky vyhledávání - "J. J. Winchenne"

Chromatographic separation of recombinant human erythropoietin isoforms

Autor: A. Ahaded, J. J. Winchenne, J.-P. Cartron, A Gokana, Patrick Lambin, A Ben-Ghanem

Publikováno v: Journal of Chromatography A. 791:109-118

Erythropoietin (EPO) is the main regulator of erythropoiesis. The human glycoprotein hormone is heterogeneous when analyzed by isoelectric focusing (IEF). We investigated the possibility of fractionating EPO isoforms using different chromatographic m

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::71c12eb1783204689061bffbc095fa6b
https://doi.org/10.1016/s0021-9673(97)00766-8

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Purification and biological activity of a recombinant human erythropoietin produced by lymphoblastoid cells

Autor: M. Dubarry, J.-P. Cartron, Patrick Lambin, S. Chrétien, P. Rouger, C. T. Craescu, J.P. Le Caer, Claude Lopez, A. Ben Ghanem, J. J. Winchenne, N. Casadevall

Publikováno v: Preparative biochemistry. 24(2)

A recombinant human erythropoietin (rH-EPO) was obtained from the culture supernatants of human B-lymphoblastoid cells transfected by the human EPO gene. rH-EPO was purified by a two-step method based on immunoaffinity and ion exchange chromatography

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0118b71ef4ae7f39d41fdb7d29278377
https://pubmed.ncbi.nlm.nih.gov/8072955

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Preparation and biological properties of monoclonal antibodies against human erythropoietin

Autor: S. Pallu, P. Mayeux, G.A. Boffa, Patrick Lambin, N. Casadevall, N. Raymonjean, D. Goossens, J.-P. Cartron, A. Ben Ghanem, J. J. Winchenne

Publikováno v: Hybridoma. 12(5)

Several monoclonal antibodies (Mabs) against human erythropoietin (H-EPO) were obtained by fusion of myeloma cells with splenocytes from mice immunized with purified recombinant H-EPO. Three Mabs (D7, E14 and E73) were selected by radioimmunoprecipit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::428ffd37b86332492ffe0ae9be837e67
https://pubmed.ncbi.nlm.nih.gov/8300136

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Rat serum factors inhibiting the G1-S transition in hepatocytes. II. Properties of the low molecular weight factor

Autor: E, Le Rumeur, J J, Winchenne, G A, Boffa, C, Nadal

Publikováno v: Cell and tissue kinetics. 16(4)

The properties were investigated of low molecular weight factors acting on the G1-S transition of baby rat hepatocytes. These factors were produced by incubating adult rat serum with trypsin or a 100,000 g liver microsomal fraction, and isolated by u

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::a45c75e0e9625beee7e05ed4f3646c55
https://pubmed.ncbi.nlm.nih.gov/6861192

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[Release from human alpha 2-macroglobulin of a peptide inhibitor of G1-S transition of hepatocytes in vivo. Role of trypsin, thrombin and various chemical agents]

Autor: P, Lambin, C, Nadal, J J, Winchenne, G A, Boffa

Publikováno v: Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie. 304(19)

Inhibitory effect on the G1-S transition of hepatocytes in vivo was measured in the ultrafiltrates of alpha 2 M/trypsin and alpha 2 M/thrombin complexes (alpha 2 macroglobulin: alpha 2 M). Untreated human alpha 2 M activity corresponds to one inhibit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::4a3d7c39d34572faefc0888c93338ac4
https://pubmed.ncbi.nlm.nih.gov/2438019

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[Detection and purification of a liver microsomal enzyme inducing the release of a glycopeptide inhibitor of hepatocyte proliferation from human alpha 2 macroglobulin]

Autor: C, Leborgne, C, Nadal, J J, Winchenne, G A, Boffa

Publikováno v: Comptes rendus des seances de la Societe de biologie et de ses filiales. 181(2)

The interaction rat liver microsomes/human alpha 2 macroglobulin releases in vivo an inhibitory peptide of the hepatocyte proliferation. Treatment of Triton X 100 on adult rat liver microsomes enables the solubilisation of a proteolytic enzyme. Its p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::a1ee08a000e3e59e673df1702c5cca37
https://pubmed.ncbi.nlm.nih.gov/2441826

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Effects of conditioned medium from bone marrow of normal and Ara-C-treated mice on hemoglobin synthesis

Autor: G A, Boffa, D, Dumenil, N, Lucien, J J, Winchenne, E, Frindel

Publikováno v: Experimental hematology. 10(8)

Differentiation of CFUs toward erythropoiesis has been demonstrated in mice treated with a single dose of Ara-C, and this preferential differentiation was shown to be under the influence of diffusible factors. The aim of the work reported here was to

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::0e92a1a69b36a5adf49dbdf680413a8c
https://pubmed.ncbi.nlm.nih.gov/7140868

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[Lipoprotein-phospholipase interactions in the course of hemolysis caused by the venom of Vipera aspis]

Autor: G A, Boffa, M C, Boffa, M, Burstein, J J, Winchenne

Publikováno v: Comptes rendus des seances de la Societe de biologie et de ses filiales. 165(12)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::167ec6dc541a173f5183f61783257a2a
https://pubmed.ncbi.nlm.nih.gov/4263353

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[Steroid-protein interactions in human, amphibian and Cyclostoma sera. Analytical ion exchange chromatography]

Autor: G A, Boffa, B, Martin, J J, Winchenne, R, Ozon

Publikováno v: Biochimie. 54(9)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::a7108a3f789f0b66a0db778f87a21c1e
https://pubmed.ncbi.nlm.nih.gov/4655022

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