Výsledky vyhledávání - "J. J. Scocca"

Interaction of integration host factor from Escherichia coli with the integration region of the Haemophilus influenzae bacteriophage HP1

Autor: Eun Seong Hwang, J J Scocca

Publikováno v: Journal of Bacteriology. 172:4852-4860

The specific DNA-binding protein integration host factor (IHF) of Escherichia coli stimulates the site-specific recombination reaction between the attP site of bacteriophage HP1 and the attB site of its host, Haemophilus influenzae, in vitro and also

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c8d1569b1e9f954297a999c627552fdb
https://doi.org/10.1128/jb.172.9.4852-4860.1990

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Factors influencing the specific interaction of Neisseria gonorrhoeae with transforming DNA

Autor: Steven D. Goodman, J J Scocca

Publikováno v: Journal of Bacteriology. 173:5921-5923

The specific interaction of transformable Neisseria gonorrhoeae with DNA depends on the recognition of specific 10-residue target sequences. The relative affinity for DNA between 3 and 17 kb in size appears to be linearly related to the frequency of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::933b36cdc84c4b7a80e9e9a4bae86e49
https://doi.org/10.1128/jb.173.18.5921-5923.1991

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Binding sites for bacteriophage HP1 integrase on its DNA substrates

Autor: J M, Hakimi, J J, Scocca

Publikováno v: The Journal of biological chemistry. 269(33)

The temperate phage HP1 integrates its genome into the chromosome of Haemophilus influenzae by site-specific recombination between host and phage DNA segments, the attachment sites. This reaction is promoted by the HP1-encoded integrase. The interact

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::23fa6be137ed52023d0be922f25f1dba
https://pubmed.ncbi.nlm.nih.gov/8063759

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A method of cloning multiple direct repeats of a DNA segment

Autor: E S, Hwang, J J, Scocca

Publikováno v: BioTechniques. 14(5)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::56986850441f168456eb47c0b2377be3
https://pubmed.ncbi.nlm.nih.gov/8512700

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Site-specific integration of the Haemophilus influenzae bacteriophage HP1: location of the boundaries of the phage attachment site

Autor: Michael A. Hauser, J J Scocca

Plasmids containing DNA segments from the attachment region of phage HP1 were constructed and tested for the ability to replace the phage attachment site substrate in site-specific recombination reactions. The distance separating the boundaries of th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::db7f81214c42ce89089fc9e9c7a8ed67
https://europepmc.org/articles/PMC207650/

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Site-specific integration of the Haemophilus influenzae bacteriophage HP1. Identification of the points of recombinational strand exchange and the limits of the host attachment site

Autor: M A, Hauser, J J, Scocca

Publikováno v: The Journal of biological chemistry. 267(10)

Isotopic transfer experiments and boundary replacement studies were used to define the size and cleavage points of the Haemophilus influenzae attB site for phage HP1 integration. The points of strand cleavage and transfer were separated by 5' extensi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::285fce25eb621165cd4c6e2e0acfcbbb
https://pubmed.ncbi.nlm.nih.gov/1551893

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Site-specific recombination between cloned attP and attB sites from the Haemophilus influenzae bacteriophage HP1 propagated in recombination-deficient Escherichia coli

Autor: J H Astumian, J J Scocca, A S Waldman

Publikováno v: Journal of Bacteriology. 171:1747-1750

Plasmids were constructed which contain both attP and attB DNA segments derived from the insertion sites of the lysogenic bacteriophage HP1 and its host, Haemophilus influenzae. Similar plasmids containing the two junction segments (attL and attR reg

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a55622ed504e61fb1e116e683ef57d3
https://doi.org/10.1128/jb.171.3.1747-1750.1989

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Nucleotide sequence and expression of the gene for the site-specific integration protein from bacteriophage HP1 of Haemophilus influenzae

Autor: J J Scocca, Steven D. Goodman

Publikováno v: Journal of Bacteriology. 171:4232-4240

The nucleotide sequence of the leftmost 2,363 base pairs of the HP1 genome, which includes the attachment site (attP) and the integration region, was determined. This sequence contained an open reading frame encoding a 337-residue polypeptide, which

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1207bf350706f9353504abbb3375ec39
https://doi.org/10.1128/jb.171.8.4232-4240.1989

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Haemophilus influenzae periplasmic protein which binds deoxyribonucleic acid: properties and possible participation in genetic transformation

Autor: S L Sutrina, J J Scocca

Publikováno v: Journal of Bacteriology. 139:1021-1027

A protein which binds to either single-stranded or double-stranded deoxyribonucleic acid (DNA) but not to ribonucleic acid has been isolated by osmotic shock treatment of growing cells. This periplasmic protein differs from the principal intracellula

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e0dfc6fd0fd6f7f00275a6cb859915e8
https://doi.org/10.1128/jb.139.3.1021-1027.1979

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