Zobrazeno 1 - 10
of 15
pro vyhledávání: '"J. H. Waterborg"'
Publikováno v:
DNA Sequence. 6:137-146
One genomic and six cDNA clones for the replacement histone H3.2 protein of alfalfa (Medicago sativa) were isolated and sequenced. By gene organization they represent 3 distinct genes. PCR methods were used to confirm that only three intron-bearing h
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::82a82c12a70e2ebe3ffa615a451bea4e
https://doi.org/10.3109/10425179609010201
https://doi.org/10.3109/10425179609010201
Autor:
J H, Waterborg, H R, Matthews
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2
The Burton assay for DNA is a colorimetric procedure for measuring the deoxyribose moiety of DNA. It is reasonably specific for deoxyribose, although very high concentrations of ribose (from RNA) or sucrose must be avoided. The method can be used on
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::79fc3b33cfdfe115c988f53a75fcfed1
https://pubmed.ncbi.nlm.nih.gov/21374165
https://pubmed.ncbi.nlm.nih.gov/21374165
Autor:
J H, Waterborg, H R, Matthews
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1
Several methods are available for recovering proteins electrophoretically from polyacrylamide gels directly after electrophoresis or after staining for protein (see, for example, Chapter 19 ). Fluorography (see Chapter 17 ) is used to detect small am
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::75b15a3e86a8c8979c795ed076a7e0b0
https://pubmed.ncbi.nlm.nih.gov/20512685
https://pubmed.ncbi.nlm.nih.gov/20512685
Autor:
J H, Waterborg, H R, Matthews
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1
The analytical power of acrylamide gel electrophoresis is one of the keys of modern protein chemistry. It is not surprising, therefore, that many methods have been described for converting that analytical power into a preparative tool. None of the av
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::966e6e5d6b68d3ada65af9601329dac6
https://pubmed.ncbi.nlm.nih.gov/20512686
https://pubmed.ncbi.nlm.nih.gov/20512686
Autor:
J H, Waterborg, H R, Matthews
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1
The most accurate method of determining protein concentration is probably acid hydrolysis followed by amino acid analysis. Most other methods are sensitive to the amino acid composition of the protein and absolute concentrations cannot be obtained. T
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::6c5764cec24ae587b95044f23016e9e9
https://pubmed.ncbi.nlm.nih.gov/20512668
https://pubmed.ncbi.nlm.nih.gov/20512668
Publikováno v:
Plant molecular biology. 45(1)
Intron-bearing replacement histone H3 genes in Arabidopsis and other plants are highly and constitutively expressed. We demonstrate that the introns located within the 5'-untranslated regions (5'-UTR) of the two Arabidopsis replacement H3 genes will
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::417ea28db7faaaa639a027bbee4980b0
https://pubmed.ncbi.nlm.nih.gov/11247603
https://pubmed.ncbi.nlm.nih.gov/11247603
Autor:
J H, Waterborg, H R, Matthews
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 32
Fluorography is the term used for the process of determining radioactivity in gels and other media by a combination of fluorescence and photography. Since most of the radiation of a low energy emitter will largely be absorbed by the gel, in the techn
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::c23d92ec36a6015bea29255920b887bd
https://pubmed.ncbi.nlm.nih.gov/7951720
https://pubmed.ncbi.nlm.nih.gov/7951720
Autor:
J H, Waterborg
Publikováno v:
The Journal of biological chemistry. 268(7)
Histone synthesis in alfalfa tissue culture cells was studied by labeling with tritiated lysine, purification of histone proteins by reversed-phase high pressure liquid chromatography, and fluorography of acid/urea/Triton X-100 polyacrylamide gels. M
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::7c0275e57b5b0830fc3726a1083e03fe
https://pubmed.ncbi.nlm.nih.gov/8444869
https://pubmed.ncbi.nlm.nih.gov/8444869
Autor:
J H, Waterborg
Publikováno v:
The Journal of biological chemistry. 268(7)
Dynamic lysine methylation of histone H3 in alfalfa tissue culture cells was studied by labeling with tritiated methionine, purification of variants H3.1 and H3.2 by reversed-phase high pressure liquid chromatography and amino acid analysis. Mono- an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::c4511c9ab7ecddf317db7e91733925f2
https://pubmed.ncbi.nlm.nih.gov/8444870
https://pubmed.ncbi.nlm.nih.gov/8444870
Autor:
J H, Waterborg
Publikováno v:
The Journal of biological chemistry. 265(28)
Analysis of acetylation in the two histone H3 variants of alfalfa by acid/urea/Triton-polyacrylamide gel electrophoresis has established that the minor variant H3.2 has a 2-fold higher level of acetylation than the major variant H3.1. Purification an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::ce8a0bd614bf7dba05a4c9cdf0eb95d0
https://pubmed.ncbi.nlm.nih.gov/2211618
https://pubmed.ncbi.nlm.nih.gov/2211618