Zobrazeno 1 - 10
of 18
pro vyhledávání: '"J. Brian Robertson"'
Publikováno v:
mSphere, Vol 8, Iss 4 (2023)
ABSTRACT Scheffersomyces stipitis is a yeast that robustly ferments the 5-carbon sugar xylose, making the yeast a valuable candidate for lignocellulosic ethanol fermentation. However, the non-canonical codon usage of S. stipitis is an obstacle for im
Externí odkaz:
https://doaj.org/article/088a02bbf89d459daf120e818cdfd1f5
Autor:
John S. O’ Neill, Nathaniel P. Hoyle, J. Brian Robertson, Rachel S. Edgar, Andrew D. Beale, Sew Y. Peak-Chew, Jason Day, Ana S. H. Costa, Christian Frezza, Helen C. Causton
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
Yeast exhibit oscillations that share features with circadian rhythms. The authors show that bioenergetic constraints promote oscillatory behaviour: resources are stored until supplies can support translational bursting, this is licensed by ion trans
Externí odkaz:
https://doaj.org/article/5f80bcd8c3434476af92702098c2e29a
Autor:
John S. O’Neill, Nathaniel P. Hoyle, J. Brian Robertson, Rachel S. Edgar, Andrew D. Beale, Sew Y. Peak-Chew, Jason Day, Ana S. H. Costa, Christian Frezza, Helen C. Causton
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-1 (2021)
Externí odkaz:
https://doaj.org/article/3d8511efe6754e5abea861c8a3360cef
Publikováno v:
Extremophiles
Haloarchaea have evolved to thrive in hypersaline environments. Haloferax volcanii is of particular interest due to its genetic tractability; however, few in vivo reporters exist for halophiles. Haloarchaeal proteins evolved characteristics that prom
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::277532ba5e8696415ec3597ceed73756
https://doi.org/10.1007/s00792-020-01193-x
https://doi.org/10.1007/s00792-020-01193-x
Autor:
J. Brian Robertson, Andrew D. Beale, Rachel S. Edgar, Ana S. H. Costa, Helen C. Causton, Nathaniel P. Hoyle, John S. O’ Neill, Christian Frezza, Jason Day, Sew Y. Peak-Chew
Publikováno v:
Nature Communications
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
Yeast physiology is temporally regulated, this becomes apparent under nutrient-limited conditions and results in respiratory oscillations (YROs). YROs share features with circadian rhythms and interact with, but are independent of, the cell division
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::35c7644ad361199977c3ec3fc33c80c5
Publikováno v:
Bioluminescence ISBN: 9781493938117
"pHlash" is a novel bioluminescence-based pH sensor for measuring intracellular pH, which is developed based on Bioluminescence Resonance Energy Transfer (BRET). pHlash is a fusion protein between a mutant of Renilla luciferase (RLuc) and a Venus flu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5129c1e96ef40d327aaf8d37fb2b862a
https://doi.org/10.1007/978-1-4939-3813-1_9
https://doi.org/10.1007/978-1-4939-3813-1_9
Publikováno v:
Applied Biochemistry and Biotechnology. 156:59-75
Four decades of work have clearly established the existence of autonomous oscillations in budding yeast culture across a range of operational parameters and in a few strains. Autonomous oscillations impact substrate conversion to biomass and products
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::60e67d9e05ac14eb0cd89011f8167016
https://doi.org/10.1007/s12010-008-8486-7
https://doi.org/10.1007/s12010-008-8486-7
Luciferase is a useful, noninvasive reporter of gene regulation that can be continuously monitored over long periods of time; however, its use is problematic in fast-growing microbes like bacteria and yeast because rapidly changing cell numbers and m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e85e6abd82f9e645c6826721ca6a8dd0
https://europepmc.org/articles/PMC4542259/
https://europepmc.org/articles/PMC4542259/
Autor:
Kevin Qin, Liwei Jiang, William H. Hofmeister, Karl J. Aufderheide, Carl Hirschie Johnson, Gus A. Wright, Yingjun Yan, J. Brian Robertson, M. Shane Hutson, W. Tyler McCleery, Alessandro Ustione, Chris Janetopoulos, Alexander Terekhov, Lino Costa, David W. Piston, John J. Fellenstein, John P. Wikswo
Publikováno v:
Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada. 20(1)
A microcompressor is a precision mechanical device that flattens and immobilizes living cells and small organisms for optical microscopy, allowing enhanced visualization of sub-cellular structures and organelles. We have developed an easily fabricate
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1e6d884a7ab647a24f993ef9303f2924
https://pubmed.ncbi.nlm.nih.gov/24444078
https://pubmed.ncbi.nlm.nih.gov/24444078
Publikováno v:
PLoS ONE, Vol 7, Iss 8, p e43072 (2012)
PLoS ONE
PLoS ONE
We report the development of a genetically encodable and ratiometic pH probe named "pHlash" that utilizes Bioluminescence Resonance Energy Transfer (BRET) rather than fluorescence excitation. The pHlash sensor-composed of a donor luciferase that is g
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c1ba69db907d6e7d2c0de830e643ff1d
https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22905204/?tool=EBI
https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22905204/?tool=EBI