Zobrazeno 1 - 10
of 39
pro vyhledávání: '"J. B. Alexander Ross,‡ and"'
Autor:
William R. Laws, Wolfgang Pfleiderer, Alexander S. Chin, Eleanore Seibert, Roman Osman, J. B. Alexander Ross, Mary E. Hawkins
Publikováno v:
The Journal of Physical Chemistry A. 107:178-185
Fluorescent nucleic acid analogues are valuable tools for studying DNA dynamics, protein−DNA interactions and nucleotide-dependent enzyme activities. The recent development of guanine analogues based on the isoxanthopterin structure offers new oppo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::753327b6ff162be6729387d57198bf41
https://doi.org/10.1021/jp026904j
https://doi.org/10.1021/jp026904j
Publikováno v:
Biochemistry. 41:10976-10984
Uracil DNA glycosylase (UDG) is a base excision repair enzyme that specifically recognizes and removes uracil from double- or single-stranded DNA. The efficiency of the enzyme depends on the DNA sequence surrounding the uracil. Crystal structures of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c38579e20aaeac28cf5e565cdecfef4c
https://doi.org/10.1021/bi026121o
https://doi.org/10.1021/bi026121o
Publikováno v:
Journal of Molecular Biology. 316:531-546
Interactions between the Escherichia coli cytidine repressor protein (CytR) and its operator sites at the different promoters that comprise the CytR regulon, play an important role in the regulation of these promoters. The natural operators are palin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::75e9622a4915a776b745a473b429ed1c
https://doi.org/10.1006/jmbi.2001.5302
https://doi.org/10.1006/jmbi.2001.5302
Autor:
Deborah B. Stone, Linda A. Luck, Donald F. Senear, J. B. Alexander Ross, Robert A. Mendelson, Elena Rusinova
Publikováno v:
Analytical Biochemistry. 300:77-86
Three different methods to quantitate tryptophan (Trp) analogue incorporation into recombinant proteins are described: first, spectroscopic analysis based on a linear combination of the absorption spectra of the aromatic residues in the denatured Trp
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f21dfd6dd5b769af5b9cb5748835af56
https://doi.org/10.1006/abio.2001.5441
https://doi.org/10.1006/abio.2001.5441
Publikováno v:
International Journal of Quantum Chemistry. 88:28-33
6-Methylisoxanthopterin (6-MI) is a fluorescent guanine analog that is suitable for studying deoxyribonucleic acid (DNA) structure and dynamics. To aid in the interpretation of spectroscopic data, we have carried out an ab initio quantum mechanical i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4e0364271e0a79f7a8b17e77c0663205
https://doi.org/10.1002/qua.10075
https://doi.org/10.1002/qua.10075
Publikováno v:
The Journal of Physical Chemistry A. 105:190-197
2-Aminopurine is a highly fluorescent analogue of adenine that can be incorporated synthetically into DNA with little perturbation of the native double-helical structure. The sensitive dependence of the quantum yield of this fluorophore on nucleic ac
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e3acfbd019832aedea31a7f6727cd244
https://doi.org/10.1021/jp002413s
https://doi.org/10.1021/jp002413s
Publikováno v:
Analytical Chemistry. 72:3532-3538
Thermal lens spectroscopy was combined with total internal reflectance spectroscopy to develop a novel, highly sensitive analytical method that can detect nonfluorescent as well as fluorescent analytes at surfaces and interfaces. It was verified that
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd6203e271e0591a7e7252f259a1abfb
https://doi.org/10.1021/ac991271z
https://doi.org/10.1021/ac991271z
Publikováno v:
Journal of Fluorescence. 9:379-390
Time-resolved fluorescence anisotropy is an invaluable method for investigating the internal and rotational dynamics of biomolecules. The range of rotational motions detectable by anisotropy decay is limited by the fluorescence lifetime; typically, a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7e892e23f74715db12d58ac51125bdcc
https://doi.org/10.1023/a:1020504511962
https://doi.org/10.1023/a:1020504511962
Publikováno v:
Biophysical Journal. 75(5):2564-2573
Many biological systems have multiple fluorophores that experience multiple depolarizing motions, requiring multiple lifetimes and correlation times to define the fluorescence intensity and anisotropy decays, respectively. To simplify analyses, an as
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc94482b88183664cb8ed5861e19e76b
Publikováno v:
Methods. 16:3-20
The cooperative assembly of protein-DNA complexes is a widespread phenomenon that is of particular significance to transcriptional regulation. Assembly of these complexes is controlled by the chemistry of the macromolecular interactions. In this sens
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9da3ab28b4e5ee2f4b5acc7b141be68c
https://doi.org/10.1006/meth.1998.0641
https://doi.org/10.1006/meth.1998.0641