QuantiLyseTM: Reliable DNA Amplification from Single Cells

Autor: Kenneth E. Pierce, John E. Rice, J. Aquiles Sanchez, Lawrence J. Wangh

Publikováno v: BioTechniques, Vol 32, Iss 5, Pp 1106-1111 (2002)

Amplification of DNA sequences from single cells via PCR is increasingly used in basic research and clinical diagnostics but remains technically difficult. We have developed a cell lysis protocol that uses an optimized proteinase K solution, named Qu

Externí odkaz: https://doaj.org/article/c9b59e34fb834c18b72673829c8b4ecd

Validation of FASTFISH-ID : a new commercial platform for rapid fish species authentication via universal closed-tube barcoding

Autor: John Rice, Stefano Mariani, Maleeka Singh, Christopher T. Elliott, Amanda M. Naaum, J. Aquiles Sanchez, Zoe Bleicher, Marine Cusa, Robert Hanner, Lawrence J. Wangh, Ian Goodhead, Sarah J. Helyar

Publikováno v: Naaum, A M, Cusa, M, Singh, M, Bleicher, Z, Elliott, C, Goodhead, I B, Hanner, R H, Helyar, S J, Mariani, S, Rice, J E, Wangh, L J & Sanchez, J A 2021, ' Validation of FASTFISH-ID: A new commercial platform for rapid fish species authentication via universal closed-tube barcoding ', Food Research International, vol. 141, 110035 . https://doi.org/10.1016/j.foodres.2020.110035

Seafood represents up to 20% of animal protein consumption in global food consumption and is a critical dietary and income resource for the world's population. Currently, over 30% of marine fish stocks are harvested at unsustainable levels, and the i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::72248fa809890a705091641cd77d14c5

Linear-After-The-Exponential (LATE)–PCR: An advanced method of asymmetric PCR and its uses in quantitative real-time analysis

Autor: John Rice, J. Aquiles Sanchez, Kenneth Pierce, Lawrence J. Wangh

Publikováno v: Proceedings of the National Academy of Sciences. 101:1933-1938

Conventional asymmetric PCR is inefficient and difficult to optimize because limiting the concentration of one primer lowers its melting temperature below the reaction annealing temperature. Linear-After-The-Exponential (LATE)–PCR describes a new p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c2d7b31e62fe43d6c0bfde3fff739492
https://doi.org/10.1073/pnas.0305476101

Oxidative Damage is not a Major Contributor to AZT-Induced Mitochondrial Mutations

Autor: Ravigadevi Sambanthamurthi, Lawrence J. Wangh, Adam E. Osborne, J. Aquiles Sanchez, Kenneth C. Hayes

Publikováno v: Journal of AIDS & Clinical Research.

Addition of clinically-relevant levels of 3′-Azido-3′-deoxythymidine (AZT) to cultured HepG2 cells increases the number of reactive radical species (reactive oxygen and nitrogen species [ROS and RNS]) as well as random mutations in mitochondrial

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::5cc707f183fa51f6f803b1432bafc3a5
https://doi.org/10.4172/2155-6113.1000441

Kinetic Hairpin Oligonucleotide Blockers for Selective Amplification of Rare Mutations

Autor: Lawrence J. Wangh, J. Aquiles Sanchez, Yanwei Jia

Publikováno v: Scientific Reports

Detection of rare mutant alleles in an excess of wild type alleles is increasingly important in cancer diagnosis. Several methods for selective amplification of a mutant allele via the polymerase chain reaction (PCR) have been reported, but each of t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b33ce5481a89a3e15375a014ea0141c6