Zobrazeno 1 - 10
of 12
pro vyhledávání: '"J W Pflugrath"'
Autor:
Matthew S Kelker, Colin Berry, Steven L Evans, Reetal Pai, David G McCaskill, Nick X Wang, Joshua C Russell, Matthew D Baker, Cheng Yang, J W Pflugrath, Matthew Wade, Tim J Wess, Kenneth E Narva
Publikováno v:
PLoS ONE, Vol 9, Iss 11, p e112555 (2014)
Bacillus thuringiensis strains are well known for the production of insecticidal proteins upon sporulation and these proteins are deposited in parasporal crystalline inclusions. The majority of these insect-specific toxins exhibit three domains in th
Externí odkaz:
https://doaj.org/article/a0cfcbe4301a4331aaef093959c70418
Autor:
J. W. Pflugrath
Publikováno v:
Acta Crystallographica. Section F, Structural Biology Communications
Current methods, reagents and experimental hardware for successfully and reproducibly flash-cooling macromolecular crystals to cryogenic temperatures for X-ray diffraction data collection are reviewed.
Cryocrystallography is an indispensable tec
Cryocrystallography is an indispensable tec
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8811bdaefdf73b0cd503b30d33a80219
https://pubmed.ncbi.nlm.nih.gov/26057787
https://pubmed.ncbi.nlm.nih.gov/26057787
Autor:
Joseph D. Ferrara, Pierre Labbe, Cheng Yang, John D. Phillips, J. W. Pflugrath, Frank G. Whitby, Christopher P. Hill, Howard Robinson, James P. Kushner, Christy A. Warby
Publikováno v:
Journal of Biological Chemistry. 279:38960-38968
Coproporphyrinogen oxidase (CPO) is an essential enzyme that catalyzes the sixth step of the heme biosynthetic pathway. Unusually for heme biosynthetic enzymes, CPO exists in two evolutionarily and mechanistically distinct families, with eukaryotes a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::828446c24c774ff6e39be433a492358b
https://doi.org/10.1074/jbc.m406050200
https://doi.org/10.1074/jbc.m406050200
Autor:
David McCaskill, Joshua Russell, Colin Berry, Nick X. Wang, J. W. Pflugrath, Reetal Pai, Tim J Wess, Matthew Douglas Baker, Matthew Wade, Kenneth E. Narva, Matthew S. Kelker, Cheng Yang, Steven L. Evans
Publikováno v:
PLoS ONE
PLoS ONE, Vol 9, Iss 11, p e112555 (2014)
PLoS ONE, Vol 9, Iss 11, p e112555 (2014)
Bacillus thuringiensis strains are well known for the production of insecticidal proteins upon sporulation and these proteins are deposited in parasporal crystalline inclusions. The majority of these insect-specific toxins exhibit three domains in th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4df03bca97b18bfe4bc751dbd017c63a
https://pubmed.ncbi.nlm.nih.gov/25390338
https://pubmed.ncbi.nlm.nih.gov/25390338
Autor:
J. W. Pflugrath, Cheng Yang
Publikováno v:
Acta Crystallographica Section D Biological Crystallography. 57:1480-1490
The anomalous signal of S atoms is easily detected at the Cu Kα wavelength of a non-synchrotron source with current data-collection methods. The position of sulfur and other anomalous scatterers can be located through an anomalous difference Fourier
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::55b9588e0e6f30c755a5d15b0acf1803
https://doi.org/10.1107/s0907444901013397
https://doi.org/10.1107/s0907444901013397
Autor:
Duong T. Nguyen, W. Bryan Arendall, Joseph D. Ferrara, Zhi-Jie Liu, David S. Richardson, M. Gary Newton, Irina Kataeva, Lirong Chen, Hao Xu, Doowon Lee, J. Habel, J. W. Pflugrath, Cheng Yang, John Rose, Jane S. Richardson, Wolfram Tempel, Bi-Cheng Wang
Publikováno v:
Acta crystallographica. Section D, Biological crystallography. 61(Pt 7)
Recently, the demands of high-throughput macromolecular crystallography have driven continuous improvements in phasing methods, data-collection protocols and many other technologies. Single-wavelength anomalous scattering (SAS) phasing with chromium
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e3e1e8c04ef1579ca761b10137b86df9
https://pubmed.ncbi.nlm.nih.gov/15983419
https://pubmed.ncbi.nlm.nih.gov/15983419
Publikováno v:
Acta crystallographica. Section D, Biological crystallography. 59(Pt 11)
Anomalous scattering with soft X-ray radiation opens new possibilities in phasing for macromolecular crystallography. Anomalous scattering from S atoms collected on an in-house chromium radiation source (λ = 2.29 Å) was used to phase the X-ray diff
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e536cf50afe402c7538bba346f518778
https://pubmed.ncbi.nlm.nih.gov/14573949
https://pubmed.ncbi.nlm.nih.gov/14573949
Autor:
K. Crane, B. R. Vincent, J. W. Pflugrath, R. D. Gardner, Joseph D. Ferrara, J. M. Troup, N. D. Jones
Publikováno v:
Review of Scientific Instruments. 67:3361-3361
We have designed and built TEXRAYTM CCD detectors with properties suitable for single‐crystal data collection on both small molecules and macromolecules. Characterization and use of 70 mm×70 mm and 140 mm×140 mm aperture detectors will be discuss
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::59e32e58c62902b2ca7f514f56069494
https://doi.org/10.1063/1.1147505
https://doi.org/10.1063/1.1147505
Autor:
A. Messerschmidt, J. W. Pflugrath
Publikováno v:
Journal of Applied Crystallography. 20:306-315
Routines for crystal orientation and the prediction of expected reflections which are part of the data collection software package MADNES for area-detector diffractometer systems in macromolecular crystallography are described. This package is design
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::95846214b9785a8707eb5b0e57c92bf9
https://doi.org/10.1107/s002188988708662x
https://doi.org/10.1107/s002188988708662x
Publikováno v:
The Journal of biological chemistry. 258(22)
The ligand reactions of three binding proteins involved in bacterial transport and chemotaxis have been examined by stopped flow, rapid mixing techniques. The processes measured were: L-arabinose, D-galactose, and D-fucose binding to the Escherichia
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::e4eea155f9d389b60fbb6f240c76f46f
https://pubmed.ncbi.nlm.nih.gov/6358208
https://pubmed.ncbi.nlm.nih.gov/6358208