Výsledky vyhledávání - "J W Jacobberger"

Stoichiometry of immunocytochemical staining reactions

Autor: J W, Jacobberger

Publikováno v: Methods in cell biology. 63

This chapter reviewed and presented my biases about the factors that affect our ability to make quantitative measurements of epitope (for this chapter, equal to a protein) with monoclonal antibodies in a flow cytometric system. The discussion has ill

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::24c475b757b240ad558fee2bcaccec3d
https://pubmed.ncbi.nlm.nih.gov/11060846

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Molecular quantification of cell cycle-related gene expression at the protein level

Autor: P S, Frisa, R E, Lanford, J W, Jacobberger

Publikováno v: Cytometry. 39(1)

Immunofluorescence cytometry of antigen and DNA content provides relative measurements of the cell cycle phase distribution of a specific epitope. Measurement of correlated expression of epitopes on signaling and regulatory proteins will be useful in

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https://pubmed.ncbi.nlm.nih.gov/10655566

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Bivariate analysis of the p53 pathway to evaluate Ad-p53 gene therapy efficacy

Autor: J W, Jacobberger, R M, Sramkoski, D, Zhang, L A, Zumstein, L D, Doerksen, J A, Merritt, S A, Wright, K E, Shults

Publikováno v: Cytometry. 38(5)

Gene therapy of human tumors with adenovirus vectors presents a clinical research challenge and a potential opportunity in cancer therapy. One of the research challenges is that endpoints like tumor reduction, time to recurrence, and survival do not

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::f0c48c31dec0920bcc9a6d1b94d39a76
https://pubmed.ncbi.nlm.nih.gov/10516606

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Simultaneous detection of cyclin B1, p105, and DNA content provides complete cell cycle phase fraction analysis of cells that endoreduplicate

Autor: R M, Sramkoski, S W, Wormsley, W E, Bolton, D C, Crumpler, J W, Jacobberger

Publikováno v: Cytometry. 35(3)

DNA analysis of endoreduplicating cells is difficult because of the overlap between stem-line G2 + M cells and 4C G1 cells. Simultaneous flow cytometry of DNA and cyclin B1 analytically separates these populations. The objective here was to develop s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::b6ed3f1f2e515ab74da4b457bf9355ff
https://pubmed.ncbi.nlm.nih.gov/10082309

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Estimation of kinetic cell-cycle-related gene expression in G1 and G2 phases from immunofluorescence flow cytometry data

Autor: J W, Jacobberger, R M, Sramkoski, S B, Wormsley, W E, Bolton

Publikováno v: Cytometry. 35(3)

Flow cytometry of immunofluorescence and DNA content provides measures of cell-cycle-related gene expression (protein and/or epitope levels) for asynchronously growing cells. From these data, time-related expression through S phase can be directly me

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::1f51255618e18b14d778f9a8542a1a63
https://pubmed.ncbi.nlm.nih.gov/10082310

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Virus-cell interactions. Introduction

Autor: J M, Lehman, J W, Jacobberger

Publikováno v: Cytometry. 31(4)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::de62edec4d3e3f6149454c9363548b65
https://pubmed.ncbi.nlm.nih.gov/9551597

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Cell cycle analysis of retroviral vector gene expression during early infection

Autor: T L, Sladek, J W, Jacobberger

Publikováno v: Cytometry. 31(4)

To examine the pattern of retroviral vector gene expression during early stages of infection, a Moloney murine leukemia virus (M-MuLV)-based vector that transcribes the simian virus 40 (SV40) large T antigen (Tag) gene from the viral long terminal re

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::999e29df8d52acb209b6879f14b64bb7
https://pubmed.ncbi.nlm.nih.gov/9551598

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HIV type 1 Tat protein induces apoptosis and death in Jurkat cells

Autor: Scott F. Purvis, R M Sramkoski, Abhay H. Patki, J W Jacobberger, Michael M. Lederman

Publikováno v: AIDS research and human retroviruses. 11(4)

Jurkat cells stably expressing high levels of the HIV-1 Tat protein were generated after transfection with an Epstein-Barr virus-based episomal replicon and selection in hygromycin B. The Jurkat Tat transfectants exhibited a longer doubling time when

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8f8fc82a21f1a4017686298d6ad6f7cc
https://pubmed.ncbi.nlm.nih.gov/7632460

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Analysis of intracellular proteins

Autor: K D, Bauer, J W, Jacobberger

Publikováno v: Methods in cell biology. 41

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::ba1927db44597983474fd81f2fb46ce3
https://pubmed.ncbi.nlm.nih.gov/7532265

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Dependence of SV40 large T-antigen cell cycle regulation on T-antigen expression levels

Autor: T L, Sladek, J W, Jacobberger

Publikováno v: Oncogene. 7(7)

Simian virus 40 (SV40) large T antigen (Tag) expression results in reduced percentages of G1-phase cells and increased percentages of S- and G2+M-phase cells in exponentially growing fibroblast populations as compared with identical cell populations

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::a6037a001fbfa10ce03e29a9418bc2c0
https://pubmed.ncbi.nlm.nih.gov/1620545

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