Zobrazeno 1 - 10
of 38
pro vyhledávání: '"J S, Mort"'
Publikováno v:
European Cells & Materials, Vol 28, Pp 129-136 (2014)
Analysis of both the aggregated and non-aggregated fractions of aggrecan isolated from adult human intervertebral disc using immunoblotting with antibodies specific for the different domains constituting the aggrecan core protein or atomic force micr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0ac9a347f7b42dacab4caf37cb284edf
http://www.ecmjournal.org/journal/papers/vol028/pdf/v028a10.pdf
http://www.ecmjournal.org/journal/papers/vol028/pdf/v028a10.pdf
Publikováno v:
The EMBO Journal. 15:5492-5503
Cathepsin L is a member of the papain superfamily of cysteine proteases and, like many other proteases, it is synthesized as an inactive proenzyme. Its prosegment shows little homology to that of procathepsin B, whose structure, the first for a cyste
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d935847fe88c07ef0602ef4a74c3d39e
https://doi.org/10.1002/j.1460-2075.1996.tb00934.x
https://doi.org/10.1002/j.1460-2075.1996.tb00934.x
Publikováno v:
Journal of Biological Chemistry. 268:235-240
The pH dependence of cathepsin B-catalyzed hydrolyzes is very complex. At least seven dissociable groups are involved in the binding and hydrolysis of 7-amido-4-methyl coumarin and p-nitroaniline (pNA)-based substrates containing a P1 Arg and either
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0d094a86ea594e447203111781a1944a
https://doi.org/10.1016/s0021-9258(18)54140-5
https://doi.org/10.1016/s0021-9258(18)54140-5
Publikováno v:
Journal of Biological Chemistry. 267:4713-4721
The cysteine proteinase rat cathepsin B was expressed in yeast in an active form and was found to be heterogeneously glycosylated at the consensus sequence for N-linked oligosaccharide substitution. Purified enzyme fractions containing the highest le
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::00023b694a9db55210c53d2dbbf0cf92
https://doi.org/10.1016/s0021-9258(18)42891-8
https://doi.org/10.1016/s0021-9258(18)42891-8
Publikováno v:
The Biochemical journal. 339
A bovine nasal-cartilage culture system has been utilized to analyse the catabolic events occurring in response to interleukin-1beta over a 14-day period. An early event following the start of interleukin-1 treatment was the release of glycosaminogly
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::c0a0833a1f7bc84ade496e66064b7e7b
https://pubmed.ncbi.nlm.nih.gov/10215595
https://pubmed.ncbi.nlm.nih.gov/10215595
Autor:
J S Mort, D J Buttle
The hydrolysis of peptide bonds is an integral part of most physiological and pathological processes, yet knowledge is often lacking as to which peptide bonds are cleaved, in which protein substrates, in which order, and by which proteolytic enzymes.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::72ad7d9816c8226047191893886b402e
https://europepmc.org/articles/PMC395664/
https://europepmc.org/articles/PMC395664/
Publikováno v:
The Journal of biological chemistry. 273(8)
The autocatalytic processing of procathepsin L was investigated in vitro using purified recombinant proenzyme expressed in Pichia pastoris. Pure intermolecular processing was studied by incubating the mutant procathepsin L (C25S), which cannot autoac
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::5df73dc86353c97ef7c6b8d3cb884cc7
https://pubmed.ncbi.nlm.nih.gov/9468501
https://pubmed.ncbi.nlm.nih.gov/9468501
Publikováno v:
Clinicalexperimental metastasis. 15(2)
Cysteine proteinases, in particular cathepsins B and L, have been implicated in tumor invasion and are thought to be important mediators of metastasis. Using two clonal sublines of the Lewis lung carcinoma with distinct patterns of metastasis, we pre
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::160a47fb9ec6b669f23cf4abe155f21c
https://pubmed.ncbi.nlm.nih.gov/9062388
https://pubmed.ncbi.nlm.nih.gov/9062388
Publikováno v:
Proteins. 25(3)
Human procathepsin L has been expressed in the yeast Pichia pastoris and its inactive (Cys25Ser) and unglycosylated (Thr110Ala) mutant purified, concentrated to 4 mg/ml, and crystallized by vapor diffusion against solution containing 1.4 M (Na,K)PO4
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::8866ffbb1c4f457ce4200c02ae00a5e6
https://pubmed.ncbi.nlm.nih.gov/8844874
https://pubmed.ncbi.nlm.nih.gov/8844874
Publikováno v:
Journal of cellular physiology. 166(2)
The effects of IL-1 beta and TGF-beta on the biosynthesis of extracellular matrix structural components relative to the metalloproteinases and their inhibitor TIMP1 in human articular chondrocytes were investigated. It has been proposed that TGF-beta
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::8c2c9ded09d750ddc5ae16c2bc5f198a
https://pubmed.ncbi.nlm.nih.gov/8591995
https://pubmed.ncbi.nlm.nih.gov/8591995