Zobrazeno 1 - 7
of 7
pro vyhledávání: '"J R Ogez"'
Publikováno v:
Bioseparation. 5(2)
A high yield procedure was developed to solubilize and extract IGF-I from recombinant E. coli by adding chaotrope and disulfide reductant to alkaline fermentation broth. To enhance centrifugation performance and recovery yield, a salt/polymer aqueous
Publikováno v:
Biotechnology and applied biochemistry. 20(2)
Recombinant human insulin-like growth factor I (IGF-I), a 70-amino-acid peptide containing three disulphide bonds, produces two monomeric and several multimeric species during refolding. To optimize production of correctly folded IGF-I, conditions wh
Autor:
J R, Ogez, S E, Builder
Publikováno v:
Bioprocess technology. 10
Less than a decade ago, the use of continuous mammalian cell lines for the production of cloned proteins was considered strictly a research tool. At that time, few thought it possible to allay the many safety concerns associated with transformed cell
Publikováno v:
Journal of Biological Chemistry. 251:4551-4556
A number of potential models for the interaction of cyclic AMP with protein kinase (RC or R2C2) have been examined. These include: Model 1, the simultaneous binding of cyclic AMP and release of C (catalytic subunit) from an independent RC protomer; M
Publikováno v:
Journal of Biological Chemistry. 252:6151-6155
The chemical change responsible for the 3-oxo-4-estren-17 beta-yl acetate-dependent photoinactivation of delta 5-3-ketosteroid isomerase has been identified by amino acid analysis and amino acid sequencing. Amino acid analysis of the enzyme and its p
Publikováno v:
The Journal of biological chemistry. 252(17)
The chemical change responsible for the 3-oxo-4-estren-17 beta-yl acetate-dependent photoinactivation of delta 5-3-ketosteroid isomerase has been identified by amino acid analysis and amino acid sequencing. Amino acid analysis of the enzyme and its p
Autor:
J R, Ogez, I H, Segel
Publikováno v:
The Journal of biological chemistry. 251(15)
A number of potential models for the interaction of cyclic AMP with protein kinase (RC or R2C2) have been examined. These include: Model 1, the simultaneous binding of cyclic AMP and release of C (catalytic subunit) from an independent RC protomer; M