Zobrazeno 1 - 10
of 54
pro vyhledávání: '"J R, Zysk"'
Publikováno v:
Molecular Endocrinology
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ebd5355d20c6ea1bcf281b127519734c
https://doi.org/10.1201/9781003076926-7
https://doi.org/10.1201/9781003076926-7
Autor:
B Johnson, Charles E. Lyons, J R Zysk, Bruce D. Gaylinn, Michael O. Thorner, W R Baumbach, Cecil Mark Eppler
Publikováno v:
Biochemical and Biophysical Research Communications. 221:133-139
The receptor for growth hormone-releasing hormone (GHRH) has been purified from bovine pituitary tissue and HEK293 cells transfected with human or porcine receptor using a retrievable biotinylated GHRH analog. Custom synthesized [His1, Nle27, Biotin-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ed00623c2e472dc1571913f4f7f1dc3c
https://doi.org/10.1006/bbrc.1996.0540
https://doi.org/10.1006/bbrc.1996.0540
Publikováno v:
Endocrinology. 136:4701-4704
A binding assay for growth hormone releasing factor (GRF) has been developed using scintillation proximity assay (SPA) technology. Binding conditions were validated by several criteria. Equilibrium binding was attained within three hours at 22 degree
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::846efa632d165fbda022e7b8c126d0ff
https://doi.org/10.1210/endo.136.10.7664692
https://doi.org/10.1210/endo.136.10.7664692
Publikováno v:
Endocrinology. 136:1323-1326
Estrogen antagonists such as ICI164,384 do not inhibit 17 beta-estradiol (E2)-dependent gene activity in yeast expressing the mammalian estrogen receptor although these compounds bind to receptors isolated from these cells. Various explanations have
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f70e91829c372704c536da30227ff27a
https://doi.org/10.1210/endo.136.3.7867588
https://doi.org/10.1210/endo.136.3.7867588
Publikováno v:
Journal of Biological Chemistry. 267:15603-15612
A somatostatin (SRIF) receptor and its associated Gi regulatory proteins was purified from GH4C1 rat pituitary cells by: 1) saturation of the membrane-bound receptor with biotinyl-NH-[Leu8,D-Trp22,Tyr25] SRIF28 (bio-S28); 2) solubilization of recepto
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fb23b02651e42452a20b598c06eb83f3
https://doi.org/10.1016/s0021-9258(19)49579-3
https://doi.org/10.1016/s0021-9258(19)49579-3
Publikováno v:
Biochemical and Biophysical Research Communications. 184:131-136
A somatostatin receptor isolated from GH 4 C 1 rat pituitary tumor-derived cells was cleaved with cyanogen bromide or cyanogen bromide+trypsin to obtain sequenceable fragments. Five unique amino acid sequences ranging from 6 to 27 amino acid residues
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::02089cdc348a13f1eb0ccaeeeb50217a
https://doi.org/10.1016/0006-291x(92)91168-p
https://doi.org/10.1016/0006-291x(92)91168-p
Autor:
J R, Zysk, W R, Baumbach
Publikováno v:
Combinatorial chemistryhigh throughput screening. 1(4)
The development of high throughput, homogeneous pharmacologic and functional assays and their implementation in screening combinatorial libraries has increased the pace of stochastic drug discovery in recent years. New, noninvasive approaches involvi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::a1af6d18f2d5d79f42cb2082c188d194
https://pubmed.ncbi.nlm.nih.gov/10499123
https://pubmed.ncbi.nlm.nih.gov/10499123
Publikováno v:
The Journal of biological chemistry. 267(22)
A somatostatin (SRIF) receptor and its associated Gi regulatory proteins was purified from GH4C1 rat pituitary cells by: 1) saturation of the membrane-bound receptor with biotinyl-NH-[Leu8,D-Trp22,Tyr25] SRIF28 (bio-S28); 2) solubilization of recepto
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::3a05ad5788627347cc2ab8d0eaa43c79
https://pubmed.ncbi.nlm.nih.gov/1353497
https://pubmed.ncbi.nlm.nih.gov/1353497
Publikováno v:
Proceedings of the National Academy of Sciences. 80:760-764
Ca2+-dependent protein phosphorylation and the role of calmodulin in this process was investigated in subcellular fractions of primary cultures of rat Sertoli cells. Significant Ca2+/calmodulin-dependent protein phosphorylation in Sertoli cells was r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::310fff949e33cf7a09df0adc9141ecab
https://doi.org/10.1073/pnas.80.3.760
https://doi.org/10.1073/pnas.80.3.760
Publikováno v:
Journal of reproduction and fertility. 45(1)
When 5-thio-D-glucose was fed to male mice at daily dose levels greater than 30 mg/kg sperm development was completely inhibited within 3 weeks and remained so without impairment of libido for the experimental period of 7 weeks. Removal of this subst
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::44f1aab70c5023cb1dae2924fd6691d7
https://pubmed.ncbi.nlm.nih.gov/1195257
https://pubmed.ncbi.nlm.nih.gov/1195257