Zobrazeno 1 - 10
of 47
pro vyhledávání: '"J R, Lakey"'
Autor:
J R, Lakey, E J, Woods, M A, Zieger, J G, Avila, W A, Geary, S L, Voytik-Harbin, J K, Critser
Publikováno v:
Cell and tissue banking. 2(4)
In vitro proliferation of isolated pancreatic islets has become an area of great interest given the scarcity of clinical islet donors and the islet mass requirements for clinical islet transplantation. Small intestinal submucosa (SIS), a naturally oc
Publikováno v:
Cell transplantation. 10(8)
Ex vivo gene transfer offers a potential means to introduce genes into cells, which may play an important role in preventing graft rejection and inducing graft tolerance. This study examined the efficiency and toxicity of several lipid-based transfec
Publikováno v:
Lancet (London, England).
Publikováno v:
Cell transplantation. 10(7)
The development of effective protocols for the low-temperature banking of pancreatic islets is an important step in islet transplantation for the treatment of type I diabetes mellitus. We have been exploring the use of islets from the newborn pig as
Publikováno v:
Cell transplantation. 10(7)
Cryopreservation of islets adds great flexibility to clinical islet transplant programs. Methods of islet cryopreservation have traditionally utilized permeating cryoprotectants contained within isotonic solutions without specifically addressing issu
Publikováno v:
Transplantation. 72(6)
Optimized conditions for survival and function of human islets must be defined if sufficient islets are to be recovered from a single human donor pancreas to reverse type-1 diabetes after isolation, cryopreservation, and transplantation. The objectiv
Publikováno v:
Transplantation. 72(4)
Islet isolation from the pancreatic tissue matrix remains highly variable. Recent evidence suggests that intrinsic human pancreatic proteases, including trypsin, may inhibit effective collagenase enzymatic activity during islet isolation, thereby imp
Autor:
A M, Shapiro, E G, Hao, J R, Lakey, W J, Yakimets, T A, Churchill, P G, Mitlianga, G K, Papadopoulos, J F, Elliott, R V, Rajotte, N M, Kneteman
Publikováno v:
Transplantation. 71(12)
The inability to diagnose early rejection of an islet allograft has previously proved to be a major impediment to progress in clinical islet transplantation. The need to detect early rejection will become even more relevant as new tolerance-inducing
Publikováno v:
Microcirculation (New York, N.Y. : 1994). 7(5)
After congestive heart failure (CHF), lung endothelial permeability responses to a number of perturbations, including acute barotrauma, angiotensin II, and thapsigargin are blunted. Our hypothesis was that similar attenuation of permeability response
Publikováno v:
Cell Transplantation, Vol 7 (1998)
Density gradient separation of islets from exocrine tissue is usually performed with Ficoll. However, this reagent adds significantly to the cost of the isolation. The aim of this study was to evaluate the performance of Dextran as a potential low-co