Zobrazeno 1 - 10
of 136
pro vyhledávání: '"J Polatnick"'
Publikováno v:
Applied and Environmental Microbiology. 39:823-827
A microassay for interferon is described which uses target cells grown in microculture wells, [3H]uridine to measure vesicular stomatitis virus replication in target cells, and a multiple automated sample harvester to collect the radioactively labele
Autor:
J Y Richmond, J Polatnick
Publikováno v:
Infection and Immunity. 13:1392-1396
Three temperature-sensitive (ts) mutants of foot-and-mouth disease virus were classified as ribonucleic acid negative and as belonging to the same complementation group when measured by virus yields and [3H] uridine incorporation in paired, mixed inf
Autor:
H. L. Bachrach, J. Polatnick
Publikováno v:
Experimental Biology and Medicine. 105:486-489
SummaryAn ultra-sensitive method of assay for ribonuclease (RNase) has been devised using a biologically active ribonucleic acid (RNA) as its substrate. Infectious RNA isolated from foot-and-mouth disease virus was exposed to suitable dilutions of RN
Autor:
Howard L. Bachrach, J. Polatnick
Publikováno v:
Analytical Biochemistry. 2:161-168
Using a microbiological assay for RNase based on the inactivation of viral RNA and capable of detecting RNase at a concentration as low as 0.1 mμg/ml, commercially available crystalline preparations of the pancreatic enzymes DNase and trypsin were f
Autor:
S H Wool, J Polatnick
Publikováno v:
Journal of Virology. 56:617-619
Electrophoretic analysis of the Golgi apparatus isolated by differential centrifugation from radiolabeled cells infected with foot-and-mouth disease virus showed about 10 protein bands. The virus-induced RNA polymerase was identified by immunoprecipi
Autor:
J. Polatnick, S.H. Wool
Publikováno v:
Journal of ultrastructure research. 80(3)
A novel structure was seen during the cell-free synthesis of poly (U) by foot-and-mouth disease virus-induced poly (U) polymerase acting on a poly (A) template. An electron-microscopy spreading procedure using benzyldimethylalkylammonium chloride vis
Publikováno v:
Developments in biological standardization. 35
Publikováno v:
Veterinary microbiology. 7(5)
Foot-and-mouth disease virus (FMDV)-induced ultrastructural changes in guinea-pig tongue, heelpad, mammary and liver tissues were examined using scanning and transmission electron microscopy. FMDV infection caused cell rounding and the release of vir
Autor:
J, Polatnick, S, Wool
Publikováno v:
Canadian journal of comparative medicine : Revue canadienne de medecine comparee. 45(3)
A serological technique using electron microscope grids coated with protein A and antiserum was able to detect foot-and- mouth disease virus particles in oesophageal-pharyngeal fluids from infected cattle without the need for prior concentration of t
Publikováno v:
The Journal of general virology. 32(3)
Foot-and-mouth disease virus (a member of the picornavirus group) RNA could be translated effectively in an S-30 extract from Ehrlich ascites tumour cells. This translation was inhibited by aurintricarboxylic acid, cycloheximide, puromycin and RNase.