Identification of calnexin as a diacylglycerol acyltransferase-2 interacting protein.

Autor: Curtis Brandt, Pamela J McFie, Huyen Vu, Paulos Chumala, George S Katselis, Scot J Stone

Publikováno v: PLoS ONE, Vol 14, Iss 1, p e0210396 (2019)

Triacylglycerol synthesis is catalyzed by acyl CoA:diacylglycerol acyltransferase-2 (DGAT2). DGAT2 is an integral membrane protein that is localized to the endoplasmic reticulum and interacts with lipid droplets. Using BioId, a method to detect proxi

Externí odkaz: https://doaj.org/article/b2acf3ebbbb84957951342e75ee9586c

A fluorescent assay to quantitatively measure in vitro acyl CoA:diacylglycerol acyltransferase activity

Autor: Pamela J. McFie, Scot J. Stone

Publikováno v: Journal of Lipid Research, Vol 52, Iss 9, Pp 1760-1764 (2011)

Triacylglycerols (TG) are the major storage form of energy in eukaryotic organisms and are synthesized primarily by acyl CoA:1,2-diacylglycerol acyltransferase (DGAT) enzymes. In vitro DGAT activity has previously been quantified by measuring the inc

Externí odkaz: https://doaj.org/article/368b7becdd8545f3b42d0987d06500c5

Diacylglycerol acyltransferase-2 contains a c-terminal sequence that interacts with lipid droplets

Autor: Shanna L. Banman, S. Stone, Pamela J. McFie

Publikováno v: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. 1863:1068-1081

Diacylglycerol acyltranferase-2 (DGAT2) is a resident protein of the endoplasmic reticulum that catalyzes the synthesis of triacylglycerol. When lipid droplet formation is stimulated by incubating cells with fatty acids, DGAT2 becomes concentrated ar

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22b4a7eeab8892766aa2d7ee7940c819
https://doi.org/10.1016/j.bbalip.2018.06.008

Membrane topology of human monoacylglycerol acyltransferase-2 and identification of regions important for its localization to the endoplasmic reticulum

Autor: Huyen Vu, Pamela J. McFie, Sabrina Izzard, Luc G. Berthiaume, Erwan Beauchamp, Youzhi Jin, S. Stone

Publikováno v: Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids
Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids, Elsevier, 2016, 1861 (9, Part A), pp.1192-1204

International audience; Acyl CoA:2-monoacylglycerol acyltransferase (MGAT)-2 has an important role in dietary fat absorption in the intestine. MGAT2 resides in the endoplasmic reticulum and catalyzes the synthesis of diacylglycerol which is then util

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::34425615a2421e35e56558074abb8348
https://doi.org/10.1016/j.bbalip.2016.06.021

The Mitochondrial Metallochaperone SCO1 Is Required to Sustain Expression of the High-Affinity Copper Transporter CTR1 and Preserve Copper Homeostasis

Autor: Scot C. Leary, Francisca Diaz, Michael J. Petris, Chris Hlynialuk, Timothy Wai, Amr M. El Zawily, Paul A. Cobine, S. Stone, Amzad Hossain, Pamela J. McFie, Lisa D. Yu, Deepa Viswanathan, Aren Boulet, Binbing Ling, Carlos T. Moraes, Zakery N. Baker

Publikováno v: Cell Reports
Cell Reports, Elsevier Inc, 2015, 10 (6), pp.933-943. ⟨10.1016/j.celrep.2015.01.019⟩
Cell Reports, Vol 10, Iss 6, Pp 933-943 (2015)

Summary Human SCO1 fulfills essential roles in cytochrome c oxidase (COX) assembly and the regulation of copper (Cu) homeostasis, yet it remains unclear why pathogenic mutations in this gene cause such clinically heterogeneous forms of disease. Here,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1ce67bbaae583bfa15162eb09dee343c
https://doi.org/10.1016/j.celrep.2015.01.019